Extracellular vesicles in disease

Specialized Pro-Resolving Mediator loaded Extracellular Vesicles Mitigate Pulmonary Inflammation

The paper develops a cell-based platform to generate Resolvin-loaded extracellular vesicles that reduce pulmonary inflammation in mice.

Exploring Size Exclusion Chromatography Columns 20 and 35 nm Pore Size Effect for Isolation of Extracellular Vesicles

This study compares 20 nm and 35 nm pore size SEC columns for EV isolation, finding that smaller pores improve yield and purity in cell culture media and plasma.

Extracellular vesicle bioactivity and potential clinical utility is determined by mesenchymal stromal cell clonal subtype

This study compares EV bioactivity and clinical utility from two clonal MSC subtypes, finding Y201 EVs show enhanced proliferation effects and in vivo efficacy compared to Y202 EVs.

Extracellular vesicles from chylomicron-treated endothelial cells drive macrophage inflammation

This study shows that chylomicron-treated endothelial cells release extracellular vesicles that drive macrophage inflammation and lipid droplet formation.

Electron microscopy visualization of cell-free mitochondrial DNA-containing extracellular vesicles in human plasma, serum, and saliva

The paper uses electron microscopy to analyze mitochondrial DNA-containing extracellular vesicles in human biofluids, showing that cf-mtDNA is often associated with double-membrane particles.

Silencing the signal: The metastasis suppressor NDRG1 disrupts exosome-mediated crosstalk in pancreatic cancer

This paper shows that NDRG1 suppresses pancreatic cancer metastasis by disrupting exosome-mediated communication between cancer cells and the tumor microenvironment.

Enrichment of Extracellular Vesicles Subsets from Diverse Biological Sources Using Conventional and Image-Based Fluorescence Activated Sorting

This paper develops and validates an optimized workflow for isolating and characterizing extracellular vesicle subsets from diverse sources using FACS and imaging techniques.

LoAR-Exo and HiAR-Exo: One-step label-free isolation of extracellular vesicles using inertial microfluidic devices

This paper introduces two inertial microfluidic devices for label-free EV isolation, showing comparable performance to standard methods in size distribution and efficiency.

Atypical antipsychotics alter microglial functions via astrocyte-derived extracellular vesicles

This study shows that atypical antipsychotics like clozapine and olanzapine alter microglial functions through astrocyte-derived extracellular vesicles by affecting phagocytosis and motility.

Phosphatidylinositol (4,5)-bisphosphate Impacts Extracellular Vesicle Shedding from C. elegans Ciliated Sensory Neurons

This study investigates how phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2) affects extracellular vesicle (EV) shedding from C. elegans ciliated sensory neurons, revealing differential impacts on EV subpopulations.

Thrombospondin Encapsulated Dense Particles accelerate wound healing and downregulate fibrotic phenotypes

The paper identifies ThrEDs, a novel type of extracellular particle that accelerates wound healing and reduces fibrotic responses in patient-derived myofibroblasts.

Endosome transcriptomics reveal trafficking of Cajal bodies into multivesicular bodies

The paper identifies nuclear small regulatory RNAs in multivesicular bodies and shows their trafficking requires ESCRT machinery but not LC3 lipidation.

Bovine colostrum-derived extracellular vesicles impair cancer cell proliferation through transcriptional repression.

This study shows bovine colostrum-derived extracellular vesicles inhibit cancer cell proliferation through transcriptional repression and chromatin remodeling without inducing cell death.

Monitoring the dynamics of exosomes through the tracking of Rab7 partners

The paper develops a tool to track exosomal cargoes by labeling Rab7 partners using proximity labeling and mass spectrometry.

Staphylococcus aureus derived extracellular vesicles trigger multiple inflammatory pathways in host cells and deliver their RNA cargo following their internalization and lysis within late endosomes

M2 Macrophage Exosomes Reverse Cardiac Functional Decline in Mice with Diet-Induced Myocardial Infarction by Suppressing Type 1 Interferon Signaling in Myeloid Cells

Deciphering lipidomics landscape of urine extracellular vesicles in alcohol use disorder patients

This study characterizes the lipidomic profile of urine extracellular vesicles in male alcohol use disorder patients, identifying FA 22:0 as a potential biomarker linked to inflammation and metabolic dysfunction.

Amniotic fluid extracellular vesicle properties evolve with gestational age and reflect fetal development

This study examines how amniotic fluid extracellular vesicles (AF-EVs) change with gestational age, showing shifts in EV subtypes and protein content that reflect fetal development.

Magnetically Labeled iPSC-Derived Extracellular Vesicles Enable MRI/MPI-Guided Regenerative Therapy for Myocardial Infarction

This study develops magnetically labeled iPSC-derived extracellular vesicles for MRI/MPI-guided myocardial infarction therapy, showing improved cardiac function and detectability.

Proteomic profiling of extracellular vesicles distinguishes prostate cancer molecular subtypes

This study uses proteomic profiling of extracellular vesicles to distinguish prostate cancer subtypes and identify subtype-specific biomarkers.

Proteomic profiling of bacterial extracellular vesicles for exploring ovarian cancer biomarkers

This study identifies bacterial EV proteins associated with ovarian cancer using proteomic profiling of ascites fluid.

Simultaneous Isolation and Characterization of Lipoprotein Classes in Plasma, Including HDL Subclasses and the Uncharacterized Dense HDL

The paper describes a method to isolate and characterize lipoprotein classes in plasma, revealing unique lipidomic, proteomic, and small RNA signatures for each class.

Substrate Stiffness Modulates Fibroblast Extracellular Vesicle Secretion via Mechanotransduction Pathways

This study shows substrate stiffness affects fibroblast EV size and composition through mechanotransduction pathways, with distinct roles for p53 and thioredoxin in different fibroblast types.

Cargo and biological properties of extracellular vesicles released from human adenovirus type 4-infected lung epithelial cells

This study characterizes the cargo and biological properties of extracellular vesicles from HAdV-E4-infected lung epithelial cells, showing they carry viral proteins, RNAs, and DNA and can initiate infection.

Nanogram of Arg 1 Delivered by extracellular vesicles restore ARG1 activity in a mouse model of ARG1-D and improve lifespan.

This paper demonstrates that extracellular vesicles carrying ARG1 can restore enzyme activity and extend lifespan in a mouse model of ARG1-D.

Oncogenic KRAS regulates secretion of extracellular vesicles and surface membrane charge via regulation of phosphatidylserine in pancreatic cancer

This study develops a microfluidic device to enrich pancreatic cancer-derived extracellular vesicles based on charge, linking KRAS status to EV secretion and surface charge.

Gangliosides Modulate the Secretion of Extracellular Vesicles and Their Misfolded Protein Cargo

This study shows gangliosides modulate EV secretion and misfolded protein cargo, with GM1 enhancing EV release and reducing mutant huntingtin burden in HD models.

Loss of ovarian hormones modulates the nucleic acid content of circulating extracellular vesicles and skeletal muscle metabolism in response to acute exercise

This study shows that loss of ovarian hormones alters EV nucleic acid content and skeletal muscle metabolism during acute exercise in female rats.

Human Breast Milk EVs Mitigate Endothelial Dysfunction: Preliminary Study

This study investigates the therapeutic potential of human breast milk EVs in mitigating endothelial dysfunction, showing reduced inflammation and improved vascular function in vitro and in mice.

Extracellular vesicles from morphine-exposed prefrontal cortex carry transcriptomic and proteomic signatures of synaptic dysfunction

This study shows that chronic morphine exposure alters the transcriptomic and proteomic content of brain-derived extracellular vesicles from the prefrontal cortex, which can reprogram recipient neurons.