Lipid metabolism and biosynthesis

Artificial endoplasmic reticulum-lipid droplet tethers facilitate lipid incorporation into lipid droplets

This study uses a dimerization-dependent fluorescent protein system to induce ER-LD membrane contact sites, showing that artificial tethering increases LD size by facilitating lipid incorporation from the ER.

Mechanism of substrate recognition and catalysis in a mammalian phosphatidylserine synthase 2

The paper determines the structure of PTDSS2 from Equus caballus in complex with PE or serine substrates, revealing substrate binding sites and PLD activity.

Activation-independent capture of free fatty acids at the bacterial cell envelope by BrtB

The paper shows that BrtB in Synechocystis captures free fatty acids at the cell envelope without activation, generating B-FAs and suggesting a new pathway for FFA incorporation.

Chromosome-scale genome of the woody oilseed crop sacha inchi elucidates the molecular basis of alpha-linolenic acid biosynthesis and triacylglycerol accumulation in seeds

This study presents a chromosome-scale genome of sacha inchi, revealing mechanisms of ALA biosynthesis and TAG accumulation through transcriptome analysis and gene expression patterns.

APOE genotypes differentially remodel the astrocytic lipiddroplet-associated proteome to shape lipid droplet dynamics

This study shows APOE genotypes alter astrocytic lipid droplet proteomes and dynamics, with APOE4 showing resistance to lipophagy, suggesting impaired clearance as a mechanism for AD risk.

On the stabilization of plant lipid droplets: Dynamic interplay between oleosins and phospholipids

This study investigates the dynamic interaction between oleosins and phospholipids in stabilizing plant lipid droplets using microfluidic systems, revealing that oleosins primarily stabilize while phospholipids destabilize the interface.

Torsins Organize CLCC1 Assembly to Safeguard ER Bilayer and Lipid Homeostasis

The paper identifies CLCC1 as a client of Torsin ATPases, showing they organize CLCC1 to maintain ER bilayer and lipid homeostasis, with implications for MASH development.

ATGL-mediated lipid droplet lipolysis promotes collective migration in Drosophila

This study shows that ATGL-mediated lipid droplet lipolysis is essential for collective migration in Drosophila border cells by regulating energy production and mitochondrial function.

Tripartite ER-Mitochondria-Lipid Droplets contact sites control adipocyte metabolic flexibility

This study shows that Seipin regulates tripartite ER-mitochondria-lipid droplet contact sites, which are critical for adipocyte lipid storage and metabolic flexibility.

Dithionite quenching of NBD-labeled lipids reveals artificial lipid droplet purity and neutral lipid surface accessibility

A pcyt-1 Allelic Series Reveals In Vivo Consequences of Reduced Phosphatidylcholine Synthesis in C. elegans

This study characterizes the effects of reduced phosphatidylcholine synthesis in C. elegans using pcyt-1 mutant alleles, revealing compensatory lipid remodeling and specific developmental vulnerabilities.

Lipid droplet isolation as a novel platform for spectroscopic investigation of cargo modifications

The paper uses Raman spectroscopy to analyze lipid droplet composition in Schwann and MPNST cells under CBD, radiation, and combined treatments, revealing treatment-specific metabolic changes.

FabF-FadM Cooperate to Recycle Fatty Acids and Rescue ΔplsX Lethality in Staphylococcus aureus

This paper identifies that mutations in fabF or fadM allow Staphylococcus aureus to bypass PlsX dependency by releasing fatty acids, leading to growth rescue but increased beta-lactam sensitivity.

Organelle bridges and nanodomain partitioning govern targeting of membrane-embedded proteins to lipid droplets

The paper shows that LD-targeted proteins are confined by nanodomains and transported via ER-LD bridges, revealing mechanisms for their selective accumulation on lipid droplets.

Dehydration promotes intracellular lipid synthesis and accumulation

This study shows dehydration increases intracellular lipid synthesis and accumulation by promoting glutamine oxidation and mitochondrial metabolic remodeling.

Structural basis of substrate recognition and membrane association by the bacterial lysyl-phosphatidylglycerol hydrolase AcvB

The paper determines the crystal structures of AcvB and its catalytic domain, revealing how they recognize and hydrolyze Lys-PG and interact with LpiA for lipid remodeling.

An optimized method to visualize lipid droplets in brain tissue demonstrates their substantial accumulation in aged brains

Polyketide Synthase-Like Functionality Acquired by Plant Fatty Acid Elongase

The paper identifies a novel fatty acid elongation pathway in Orychophragmus limprichtianus that produces keto-hydroxy fatty acids via a polyketide synthase-like mechanism.

Neuronal lipid droplets play a conserved and sex-biased role in maintaining whole-body energy homeostasis

The paper identifies neuronal lipid droplets as conserved structures that regulate energy homeostasis with sex-biased effects in flies and mice.

Oligomeric assemblies of plant biotin carboxylase revealed by cryo-EM and cross-linking

This study uses cryo-EM and cross-linking to reveal the oligomeric structure of plant biotin carboxylase, showing asymmetric dimer-of-dimers and dynamic B-domain interactions.

Spatially resolved rewiring of mitochondria-lipid droplet interactions in hepatic lipid homeostasis

Imaging of mitochondria and lipid droplets (LDs) across mouse liver lobules shows fasting, not Western diet, expands mitochondria-LD contacts; PLIN5 phosphomutants tune contacts and lipid storage.

Mitochondrial control of glycerolipid synthesis by a PEP shuttle

This study identifies SLC25A35 as a mitochondrial PEP transporter that regulates glycerolipid synthesis in lipogenic cells, with implications for metabolic diseases.

Origin of Eukaryotic Plasmalogen Biosynthesis by Horizontal Gene Transfer from Myxobacteria

The paper traces the origin of eukaryotic plasmalogen biosynthesis to horizontal gene transfer from Myxobacteria, revealing evolutionary divergence in plasmalogen synthesis across eukaryotic lineages.

CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly

This study identifies CLCC1 as a regulator of hepatic neutral lipid flux and nuclear pore complex assembly through CRISPR screens and functional analyses.

CLCC1 Governs Bilayer Equilibration at the Endoplasmic Reticulum to Maintain Cellular and Systemic Lipid Homeostasis

This study identifies CLCC1 as a key regulator of phospholipid trans-bilayer equilibration at the ER, essential for lipid homeostasis and preventing metabolic diseases.

Dynamic network regulating phosphatidic acid homeostasis revealed using membrane editing coupled to proximity labeling

The paper develops a 'Feeding-Fishing' approach to study phosphatidic acid homeostasis by combining membrane editing and proximity labeling, identifying key enzymes and lipid transfer proteins involved in PA metabolism.

Unsaturated Fatty Acids Are Required for Germline Proliferation and Membrane Structural Integrity in Caenorhabditis elegans

This study shows that unsaturated fatty acids are essential for germline proliferation, DNA replication, meiotic progression, and membrane integrity in C. elegans.

Conserved lipid metabolic reprogramming confers hypoxic and aging resilience

This study identifies lipid metabolic reprogramming as a conserved mechanism conferring hypoxic and aging resilience in Arctic ground squirrels and C. elegans.

Sec14L6 is a PS and PI4P transporter that promotes lipid droplet formation

This study identifies Sec14L6 as a PS/PI4P transporter essential for lipid droplet formation by interacting with ACSL3 and being recruited to the ER via PGRMC1.

PII interactions with BADC and BCCP proteins co-regulate lipid and nitrogen metabolism in Arabidopsis

This study shows PII interacts with BADC and BCCP proteins to regulate lipid and nitrogen metabolism in Arabidopsis by modulating ACCase activity and protein stoichiometry.