Microtubule and mitosis dynamics

The LINC complex and microtubule motors regulate the number and position of nuclei in the subperineurial glial cells of the Drosophila blood-brain barrier

Taxane-Induced Conformational Changes in the Microtubule Lattice Activate GEF-H1-Dependent RhoA Signaling

This study shows taxanes induce conformational changes in microtubules, releasing GEF-H1 and activating RhoA signaling, leading to actin remodeling.

Safeguarding Genome Integrity: Polo-like kinase Cdc5 and phosphatase Cdc14 Orchestrate Topoisomerase II-Mediated Catenane Resolution in Mitosis

This study identifies a mechanism by which Cdc5 and Cdc14 coordinate to resolve DNA catenanes through regulation of Topoisomerase II during mitosis.

Chimeras of Kinesin-6 and Kinesin-14 reveal head-neck-tail domain functions and dysfunctions that lead to aneuploidy in fission yeast

This study creates kinesin-6 and kinesin-14 chimeras in fission yeast to show that domain functions dictate motor localization, directionality, and force production, leading to aneuploidy.

Robust division orientation of cambium stem cells requires cortical division zone components but not the preprophase band

The paper investigates division orientation in cambium stem cells, finding it depends on cortical division zone components rather than the preprophase band.

A Non-Transcriptional Mitotic Function of POU/Oct Factors Ensures Spindle Stability and Chromosome Segregation

This study identifies a non-transcriptional role for POU/Oct factors in maintaining spindle stability and chromosome segregation during mitosis in both Drosophila and human cells.

Mitotic chromatin compaction tethers extrachromosomal DNA to chromosomes and prevents their mis-segregation into micronuclei

Kinetochore components are perinuclear and form condensates in C. elegans germline

The paper shows HCP-1, a kinetochore component, forms perinuclear condensates in C. elegans germline and associates with nuclear pore proteins, affecting germ cell proliferation and spindle assembly.

Slowdown of microtubule retrograde flow enables axon and dendrite development and maintenance

The paper shows that slowing microtubule retrograde flow is essential for axon and dendrite development and maintenance through a unified mechanism.

TTLL6-mediated Polyglutamylation of PurA Maintains Colonic Crypt Integrity

This study identifies TTLL6 as a key polyglutamylase in the colon, showing its role in maintaining crypt integrity and preventing colon cancer progression.

COP9 signalosome and PRMT5 methylosome complexes are essential regulators of Lis1-dynein based transport

The paper identifies COP9 signalosome and PRMT5 methylosome complexes as regulators of Lis1-dynein transport, showing their opposing roles in dynein activation and stalling.

Mammalian Cells Integrate Endoplasmic Reticulum and Nuclear Envelope signals to time mitotic entry

This study shows mammalian cells use ER and NE structure to control mitotic timing, with ER stress delaying mitosis and disrupting spindle formation.

Cellularization in chytrid fungi uses distinct mechanisms from conventional cytokinesis and cellularization in animals and yeast

This study investigates cellularization in chytrid fungi, showing it uses distinct mechanisms from conventional cytokinesis and animal yeast cellularization, involving actomyosin-driven membrane invagination and microtubule-dependent patterning.

HMMR/RHAMM recruits SACK1D/FAM83D-CK1α complex at the mitotic spindle to control spindle alignment

This study identifies HMMR's role in stabilizing the SACK1D-CK1 complex at the mitotic spindle and shows how phosphorylation drives its degradation after mitosis.

Cryo-EM structures of the Plant Augmin reveal its intertwined coiled-coil assembly, antiparallel dimerization and NEDD1 binding mechanisms

The paper determines cryo-EM structures of Arabidopsis thaliana Augmin, revealing its coiled-coil assembly, antiparallel dimerization, and NEDD1 binding mechanisms.

RB dependent transcriptional regulation at mitotic centromeres preserves genome stability

This study shows that RB regulates centromere transcription during mitosis, and its loss leads to increased RNA Pol II activity, DNA breaks, and mitotic defects.

A system for high-throughput axonal imaging of induced pluripotent stem cell-derived human i3Neurons

The paper introduces a high-throughput 3D culture system for imaging axonal processes in human iPSC-derived neurons, enabling efficient study of microtubule dynamics and cargo transport.

Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity

This study identifies BICD2 mutations linked to SMALED2 that cause dynein hyperactivity and interactome changes, contributing to disease pathology.

Chromosome-arm 17p Loss Renders Breast Cancer Cells Vulnerable to AURKB Inhibition

This study identifies Del17p as a biomarker for AURKB inhibition in breast cancer, showing increased sensitivity to AURKB inhibitors in Del17p tumors.

A mutation in vesicular acetylcholine transporter increases tubulin acetylation compromising axonal transport

This study identifies a mutation in the vesicular acetylcholine transporter (UNC-17) that increases tubulin acetylation, impairing axonal transport by disrupting interactions between UNC-104, UNC-17, and MEC-17.

Unconventional components complement the cryptic kinetochore of the ciliate Tetrahymena thermophila

This study identifies 16 kinetochore proteins in Tetrahymena thermophila, revealing both conventional and unconventional components that contribute to its unique kinetochore structure.

Human spastin functions as an ATPase-independent microtubule nucleator via ring-stack formation

This study shows human spastin functions as an ATPase-independent microtubule nucleator by forming ring-stack structures, redefining its role in microtubule dynamics and HSP pathology.

Cooperation between cortical and cytoplasmic forces shapes planar 4-cell stage embryos

The paper investigates how cortical and cytoplasmic forces coordinate to achieve planar cleavage in ascidian embryos.

The C-terminal SUMOylation-dependent regulation of αKNL2 governs its centromere targeting and interaction with CENH3

This study identifies SUMOylation sites in KNL2 that are critical for its centromere targeting and interaction with CENH3 in Arabidopsis.

Force Scaling in Active Swarm of Microtubules via Magnetic Manipulation

This paper measures force scaling in microtubule swarms driven by kinesin motors, showing quantitative scalability and linking collective organization to mechanical performance.

Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven intracellular transport along microtubules

This study shows that cytoplasmic actomyosin fluctuations enhance dynein-driven transport in C. elegans embryos by accelerating motor-cargo movement through asymmetric force responses.

Inactivation of microtubule organizing center function of the centrosome is required for neuronal development and centriole elimination

The paper shows that inactivating centrosome function is necessary for neuronal differentiation by affecting cell morphology and dendrite development in C. elegans.

Structure and mechanism of microtubule stabilization and motor regulation by MAP9

This study identifies MAP9 as a microtubule stabilizer that promotes neuronal morphogenesis and differentially regulates kinesin motor activity.

Condensin chromatin association is regulated by SMC head and hinge engagement and phosphorylation

This study investigates how ATP binding, hinge stability, and phosphorylation regulate condensin chromatin association in budding yeast using ChIP assays and kinase activity analysis.

Polarity reversal of stable microtubules during neuronal development

This study investigates how stable microtubules reorient during neuronal development in rat hippocampal neurons, revealing a shift from plus-end-out to minus-end-out orientation.