Microtubule and mitosis dynamics

Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin

The paper shows synaptic vesicles from mouse brains can sequester soluble tubulin in vitro, forming tubulin-rich regions.

Mitotic entry is controlled by the plant-specific phosphatase BSL1 and cyclin-dependent kinase B

This study identifies BSL1 as a plant-specific phosphatase that dephosphorylates CDKB1 to promote mitotic entry by counteracting phosphorylation at T14/Y15 residues.

Substrate recognition by human separase

This study determines cryoEM structures of human separase in apo and substrate-bound forms, revealing substrate recognition mechanisms and cleavage regulation.

Control of mitotic spindle disassembly through SUMO-targeted ubiquitylation of yeast kinesin-5

The paper identifies SUMOylation and Slx5 as regulators of spindle disassembly in yeast by facilitating Cin8 ubiquitylation and removal from the mitotic spindle.

Models for the architecture of the human inner kinetochore CCAN complex on centromeric α-satellite CENP-A nucleosome arrays

The paper determines cryo-EM structures of the CCAN complex bound to different DNA sequences to understand its architecture on centromeric α-satellite CENP-A nucleosome arrays.

Cryptococcus neoformans employs both PP1 and PP2A phosphatases to silence the spindle checkpoint

This study identifies PP1 and PP2A phosphatases as key regulators of spindle checkpoint silencing in Cryptococcus neoformans, revealing their recruitment mechanisms and roles in mitotic exit.

The scaffold protein PRR14L links the PP2A-TACC3 axis to mitotic fidelity and sensitivity to MPS1 inhibition

The paper identifies PRR14L as a gene affecting mitotic fidelity and sensitivity to MPS1 inhibition through a CRISPR/Cas9 screen and proximity labeling.

A single-component optogenetic toolkit for programmable control of microtubule

The paper develops a single-component optogenetic toolkit to control microtubule organization and dynamics in living systems, revealing their role in lysosomal trafficking and ER signaling.

Insects evolved a monomeric histone-fold domain in the CENP-T protein family

The paper identifies a monomeric histone-fold domain in insect CENP-T proteins, showing structural rearrangements that replace the need for a CENP-W partner.

Microtubule deacetylation drives kinesin-1 mediated mitochondrial transport accelerating breast cancer cell migration

The paper identifies SYBU as a regulator of mitochondrial transport in breast cancer cells, showing that its loss leads to increased migration via a feedback loop involving microtubule deacetylation and kinesin-1 activity.

β-catenin controls Afadin cortical recruitment to regulate planar epithelial cell division

This study identifies β-catenin's role in regulating planar spindle orientation through Afadin cortical recruitment, ensuring symmetric epithelial cell division.

Fission yeast Pdk1 kinase regulates cytokinesis and eisosomes

This study shows Pdk1 kinase localizes to eisosomes and regulates cytokinesis via Mid1 and Sid2, expanding its role beyond growth control.

POLO kinase inhibits Protein Phosphatase 1 to promote the Spindle Assembly Checkpoint and prevent aneuploidy

This study shows POLO kinase phosphorylates PP187B to inhibit its interaction with MPS1, maintaining SAC activity and preventing aneuploidy during mitosis.

The Dynein-Dynactin complex influences amnioserosa cell morphodynamics and adhesion during Drosophila dorsal closure

Kinetochore-centrosome feedback linking CENP-E and Aurora kinases controls chromosome congression

The paper shows Aurora A and CENP-E interact via a feedback loop to control chromosome congression by modulating kinetochore-microtubule attachments and spatial cues.

Molecular Grammar of Microtubule-WettingCondensates

The paper develops a protocol to predict microtubule binding of disordered proteins by identifying two key subsequences and validating them against experimental results.

ATR promotes genome instability via CENP-A eviction from centromeres under replication stress

This study shows that ATR promotes genome instability by causing CENP-A eviction from centromeres under replication stress, leading to acentric chromosomes and segregation defects.

Developmentally regulated actin-microtubule crosstalk in Drosophila oogenesis

This study shows how actin and microtubule interactions coordinate during Drosophila oogenesis, revealing their mutual regulation in cytoskeletal organization.

Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination

The paper shows that meroblastic cleavage in zebrafish embryos involves a two-step process with mechanical uncoupling and cadherin-mediated adhesion, distinct from canonical cytokinesis.

Temporal and spatial coordination of DNA segregation and cell division in an archaeon.

This study investigates how DNA segregation and cell division are coordinated in archaea, identifying regulatory checkpoints and proteins involved in ensuring accurate genome distribution.

Localization and function of septins are susceptible to epitope tagging

The paper shows that different fluorescent tags alter septin localization and function in fission yeast, highlighting the need for rigorous testing of tagged proteins.

Direct observation of interdependent and hierarchical kinetochore assembly on individual centromeres

This study directly observes the sequential and interdependent assembly of kinetochores on individual centromeres in budding yeast, revealing regulatory dependencies and a rate-limiting step in the process.

Tubulin autoregulation factors SCAPER and TTC5 recruit γ-tubulin to non-centrosomal MTOCs for neuronal microtubule nucleation and axon regeneration

Cell size reduction scales spindle elongation but not chromosome segregation in C. elegans

The paper quantifies mitotic spindle and chromosome segregation dynamics in C. elegans embryos, finding that spindle elongation scales with cell size while chromosome segregation remains invariant.

Energy conversion mechanism revealed by ATP-free and ATP-dependent walking of myosin V motor

This study shows that myosin V can perform ATP-free walking by converting strain energy from actin binding into mechanical work, similar to ATP-dependent walking.

Microtubule remodeling by the innate immune factor Trim69 compromises dynein-dependent migration of HIV virion cores towards the nucleus

The paper shows Trim69 remodels microtubules to inhibit HIV virion core migration by stalling dynein/dynactin complexes.

PP6 phosphatase and Elongator contribute to kinesin 5-dependent spindle assembly by controlling microtubule regulator levels

The paper identifies PP6 phosphatase and Elongator as regulators of kinesin 5-dependent spindle assembly by controlling microtubule regulator levels in fission yeast.

MAP4-MAP7D1 partitioning on tyrosinated-detyrosinated microtubules coordinates lysosome positioning in nutrient signalling

This study shows how MAP4 and MAP7D1 partition on different microtubule subsets to regulate lysosome positioning in response to nutrient availability.

Identification of locally activated spindle-associated proteins in oocytes uncovers a phosphatase-driven mechanism

The paper identifies spindle-associated proteins in Drosophila oocytes and reveals a phosphatase-driven mechanism for their spatial regulation.

Structural insights into mitotic-centrosome assembly

This study investigates the structural differences in mitotic-centrosome assembly between Drosophila and C. elegans, showing divergent mechanisms involving Cnn and SPD-5 proteins.