Heat shock proteins research

The proxiomes of CDJ5 and PGRL1 in Chlamydomonas reinhardtii overlap depending on the ability of CDJ5 to bind a 4Fe-4S cluster

This study investigates the function of CDJ5 in Chlamydomonas reinhardtii by comparing its proxiomes with and without a functional 4Fe-4S cluster, revealing its role in chloroplast microcompartment localization and photosynthetic processes.

Resolving the Dynamic Interplay of the Conformational States in the TPR Domain of Human AMPylase FicD

This study uses smFRET to analyze conformational dynamics of FicD's TPR motifs, showing equilibrium between TPR-in and TPR-out states influenced by ATP and BiP.

Arg80 Orchestrates a Metabolic-Translational Proteostasis Network in S. cerevisiae

The paper identifies Arg80 as a key regulator of proteostasis in S. cerevisiae, showing its role in mitigating proteotoxic stress through interactions with arginine metabolism and stress response pathways.

Subtle variations in a client protein determine bacterial Hsp90 dependence

The paper identifies specific regions in TilS proteins that determine Hsp90 dependence by comparing bacterial orthologs and their interactions with proteases.

A Chaperonin Complex Regulates Organelle Proteostasis in Malaria Parasites

This paper identifies a chaperonin complex in malaria parasites that regulates apicoplast proteostasis and shows its potential as an antimalarial drug target.

Polyol sugar osmolyte - sorbitol corrects chromosome cohesion and translational defects in cohesin muta

This study shows that sorbitol corrects chromosome cohesion and translational defects in cohesin mutants by reducing ER stress and protein misfolding.

A Regulatory Role of Molecular Chaperones in Excited States and Functions of Folded Enzymes

This study shows chaperones modulate enzyme activity in native states, expanding their role beyond protein folding.

Heat-Induced Phosphatidylserine Changes Drive HSPA1A's Plasma Membrane Localization

This study shows heat-induced phosphatidylserine (PS) increases drive HSPA1A's plasma membrane localization, with PS synthesis inhibition reducing this localization.

New Swiss-knife activities of GroEL/Hsp60 proteins

The paper identifies new enzymatic activities of GroEL/Hsp60 proteins, including thioesterase and esterase functions, and links these to palmitoylation and antibiotic resistance in M. tuberculosis.

Multiple origins, one function: evolutionary pathways of HSP70 proteins in viruses

This study identifies HSP70 homologs in diverse viruses, revealing multiple evolutionary origins and functional adaptations.

Is Hsp110 boosting the basal disaggregation activity Hsp70 by enhanced entropic pulling strokes?

This study investigates how Hsp110 enhances Hsp70's disaggregation activity through entropic pulling by increasing effective volume.

Bacterial Hsp90 promotes virulence factor production through maintenance of NRPS megaenzymes

This study shows bacterial Hsp90 stabilizes megasynthases required for virulence factor production in E. coli and P. aeruginosa.

Chaperone saturation mediates translation and protein folding efficiency

The paper shows that chaperone saturation limits protein folding homeostasis when translation efficiency is high, revealing mutual sensitivity between translation and folding efficiency.

Proteostasis Signatures in Human Diseases

This study defines proteostasis signatures across 32 human diseases, revealing disease-specific perturbations, temporal changes, and the impact of smoking on proteostasis.

Chaperone Dependency during Primary Protein Biogenesis Does Not Correlate with Chaperone Dependency during in vitro Refolding

The paper uses LiP-MS to show that chaperone dependency during in vitro refolding does not correlate with chaperone dependency during in vivo primary protein biogenesis.

Switchable client specificity in a dual functional chaperone coordinates light harvesting complex biogenesis

This study identifies cpSRP43 as a thermo-switchable chaperone that coordinates light harvesting complex biogenesis by switching between closed and open states to support LHCP biogenesis and protect Chl biosynthesis enzymes.

Organization of the Proteostasis Network of Membraneless Organelles

The paper maps the proteostasis network of membraneless organelles, revealing the role of anabolic components and molecular chaperones in regulating protein conformations.

A new FKBP51-GR-p23-Hsp902 multi-cochaperone complex identified and characterized by site-specific in-cell photocrosslinking

The paper identifies a novel multi-cochaperone complex involving GR, p23, FKBP51, and Hsp90 using site-specific in-cell photocrosslinking, revealing functional differences between FKBP51 and FKBP52.

Pervasive divergence in protein thermostability is mediated by both structural changes and cellular environments

This study shows that both structural changes and cellular environments contribute to differences in protein thermostability between two yeast species.

Computational elucidation of possible contributors to formation and stabilization of ATP-lid down-conformation in the N-terminal domain of Hsp90

This study uses molecular dynamics simulations to identify key residues and structural features contributing to lid closure in Hsp90's N-terminal domain.

A low-complexity linker as a driver of intra- and intermolecular interactions in DNAJB chaperones

This study investigates the role of the GF-linker in DNAJB chaperones, showing its involvement in autoinhibition and its interaction with Hsc70.

A lineage-specific heat-induced feedback loop controls HrcA to promote chlamydial fitness under stress

The paper identifies a heat-induced negative feedback loop in Chlamydia involving HagF and HrcA that modulates chaperone gene expression and promotes infectious elementary body formation under stress.

Disease-causing mutations in the G protein β5 β-propeller disrupt its chaperonin-mediated folding trajectory

This study uses cryo-EM and biochemistry to show how disease-causing mutations in Gβ5 disrupt its folding by CCT, leading to trapped intermediates and neurological disease.

Hsp90-dependent triage of the glucocorticoid receptor via the CHIP E3 ubiquitin ligase

This study demonstrates that Hsp90 presents the glucocorticoid receptor to the CHIP E3 ubiquitin ligase for degradation during its folding cycle.

Mechanism and energetics of JDP induced Hsp70's conformational transition towards catalytically active state

This study investigates how J-domain proteins induce conformational changes in Hsp70 to activate ATP hydrolysis through free energy simulations and network analysis.

Inhibition of protein or glutamine biosynthesis affect the activation of the light stimulated SBiP1 chaperone by dephosphorylation in Symbiodiniaceae

The paper investigates how protein and glutamine biosynthesis inhibitors affect light-induced dephosphorylation and activation of the SBiP1 chaperone in Symbiodinium microadriaticum.

AtDjB3 regulates Hsc70-1-mediated expression of the heat shock genes and thermotolerance in Arabidopsis

This study identifies AtDjB3 as a critical modulator of the heat shock response in Arabidopsis, showing it enhances thermotolerance by facilitating Hsc70-1 recruitment to protein aggregates and promoting Hsf activation.

Disulfide-mediated tetramerization of TRAP1 fosters its antioxidant and pro-neoplastic activities.

This study shows TRAP1 forms redox-sensitive tetramers via disulfide bonds, which are critical for its antioxidant and pro-neoplastic functions in cancer cells.

HSF1 remodels mitochondrial biogenesis and function in cancer cells via TIMM17A

This study identifies TIMM17A as a key mediator of HSF1-driven mitochondrial biogenesis and function in cancer cells, revealing a mechanism linking HSF1 to mitochondrial protein import and energy production.

Scouring the human Hsp70 network uncovers diverse chaperone safeguards buffering TDP-43 toxicity

The paper identifies chaperones in the human Hsp70 network that buffer TDP-43 toxicity by reducing aggregates and enhancing solubility.