Ivan Topisirović

eIF4A inhibition disrupts resistance-associated translational and metabolic programs in BRAF-mutant melanoma

This study shows eIF4A inhibition disrupts resistance mechanisms in BRAF-mutant melanoma by targeting translational and metabolic programs, improving tumor control when combined with BRAF inhibitors.

Lactate-fueled hydride transfer metabolon drives breast cancer metastasis

This study identifies a lactate-fueled metabolon involving LDHB and HTC that promotes breast cancer metastasis by reprogramming NAD metabolism.

Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics

This study shows that mitochondrial ETF insufficiency promotes cancer growth by reprogramming bioenergetics and signaling pathways in neoplastic cells.

A Chemical-Genetic Interaction Matrix Reveals Drug Mechanism and Genetic Architecture

The paper presents a chemical-genetic interaction matrix derived from CRISPR/Cas9 screens, revealing drug mechanisms and genetic architecture in human cells.

A platform for the systematic interrogation of genetic interactions in human cells: 26 gene by genome-wide double knockout screens as a proof of concept

The paper introduces a method for systematically studying genetic interactions in human cells using CRISPR/Cas9 screens and demonstrates its effectiveness through 26 gene by genome-wide knockout screens.

Targeting the translation initiation complex in therapy-resistant and metastatic melanoma

This study identifies M19-6 as a small molecule that disrupts the eIF4F complex, reducing melanoma growth and metastasis while sparing melanocytes.

Perturbations in eIF3 subunit stoichiometry alter expression of ribosomal proteins and key components of the MAPK signaling pathways.

The paper shows that altering eIF3 subunit levels affects the translatome, particularly impacting MAPK signaling and ribosomal protein expression in HeLa cells.

MTOR modulation induces selective perturbations in histone methylation which influence the anti-proliferative effects of mTOR inhibitors

This study shows that both mTOR activation and inhibition increase H3K27me3 through distinct mechanisms, and this epigenetic change correlates with the anti-proliferative effects of mTOR inhibitors.

Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress

The paper shows that cells use an adaptive pausing response to survive severe stress by pausing translation and limiting ATP use, followed by recovery through ISR signaling.

Regulatory start-stop elements in 5' untranslated regions pervasively modulate translation

The paper identifies start-stop elements in 5' UTRs that repress translation by halting ribosomes, showing their prevalence in human genes and impact on cellular processes.

The mTORC1/S6K/PDCD4/eIF4A axis determines outcome of mitosis.

The paper identifies the mTORC1/S6K/PDCD4/eIF4A axis as critical for determining cell fate during mitotic arrest, showing that disrupting this pathway can enhance the effectiveness of anti-mitotic drugs.

Estrogen receptor alpha controls gene expression via translational offsetting

This study shows estrogen receptor alpha (ER) regulates gene expression by coordinating transcription with translational offsetting, maintaining protein levels despite changes in mRNA abundance.

Generally applicable transcriptome-wide analysis of translational efficiency using anota2seq

The paper introduces anota2seq, an algorithm for analyzing translational efficiency from ribosome or polysome profiling data, revealing translational buffering and mTOR-dependent gene regulation.