Advanced Electron Microscopy Techniques and Applications

Correlative 4D-STEM Ptychography and EELS for Cryogenic Single-Particle Analysis

This paper introduces two optical geometries for correlative 4D-STEM and EELS to enable simultaneous structural and chemical analysis of biological specimens at sub-nanometre resolution.

An efficient eukaryotic cell-free expression and correlative cryo-electron tomography platform

The paper develops a method to express and visualize eukaryotic proteins using cell-free systems and cryo-electron tomography, demonstrating its application to study ASC protein filaments and their interaction with ribosomes.

Icecream: High-Fidelity Equivariant Cryo-Electron Tomography

Icecream improves cryo-ET reconstruction by addressing denoising, missing-wedge recovery, and computational efficiency using equivariant deep learning.

End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC

This paper automates repeat-target cryo-EM structure determination in CryoSPARC, achieving high-resolution results for 21 GPCR datasets with minimal human intervention.

Synapse Detection Efficiency in EM Drosophila Connectomics

The paper compares synapse detection in Drosophila EM reconstructions using SS-TEM and FIB-SEM, finding FIB-SEM detects more synapses.

Integrative Structure and Function of the Vibrio cholerae Competence Pilus Machine

The paper presents a 3.3 Å cryo-EM map of Vibrio cholerae competence pilus and a full-length pseudoatomic model of the CPM, revealing unique structural features and mechanical properties.

Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM

This paper introduces a method using Ni-NTA lipid monolayers to improve cryo-EM grid preparation, enabling high-resolution structure determination of proteins and the nucleosome.

cryoTIGER: Deep-Learning Based Tilt Interpolation Generator for Enhanced Reconstruction in Cryo Electron Tomography

cryoTIGER uses deep learning to generate intermediate tilt images in cryo-electron tomography, improving 3D reconstruction and structural recovery.

Optimization of adhesion for high throughput cryo-electron tomography of vitreous sections

This paper optimizes adhesion methods for cryo-electron tomography of vitreous sections, increasing throughput to match thin film methods and enabling high-resolution imaging of molecular complexes.

EVOH Hollow Fiber Encapsulation of Individual Caenorhabditis elegans for Transmission Electron Microscopy

This paper introduces a method to encapsulate individual C. elegans for TEM using EVOH hollow fibers, preserving specimen integrity and improving sectioning reproducibility.

ANTIDOTE: A Metadata-Driven Neural Network for Improving CryoEM 3-D Particle Sorting

ANTIDOTE improves cryoEM particle sorting by using metadata-driven neural networks, enhancing reconstruction quality and reducing manual curation efforts.

Template Learning: Deep Learning with Domain Randomization for Particle Picking in Cryo-Electron Tomography

This paper introduces Template Learning, a deep learning method for particle picking in cryo-ET that reduces reliance on annotated datasets by simulating training data.

Few-Shot Classification of Cryo-EM Micrographs Using Triplet Loss Embeddings

This paper develops a few-shot learning framework for cryo-EM micrograph classification using triplet loss embeddings, achieving competitive performance with minimal labeled examples.

Disulfide constrained Fabs overcome target size limitation for high-resolution single-particle cryo-EM

This paper introduces Rigid-Fabs, disulfide-constrained antibody fragments that enable high-resolution cryo-EM structures of small proteins by overcoming size limitations.

Correcting Non-Uniform Milling in FIB-SEM Images with Unsupervised Cross-Plane Image-to-Image Translation

This paper develops a deep learning method to correct non-uniform milling artifacts in FIB-SEM images using unsupervised cross-plane image-to-image translation.

Alternating Angle Milling Suppresses Streaking Artifacts in FIB-SEM Imaging

The paper introduces an alternating angle milling technique to reduce streaking artifacts in FIB-SEM imaging, improving structural data quality by minimizing non-uniform material removal.

Exploring the potential for scanning electron microscopy/focused ion beam - based diffraction for screening cryo-transmission electron microscopy samples

The paper proposes using electron backscatter diffraction in a cryo-SEM to assess ice crystallinity in cryo-TEM samples before preparation, enabling better targeting of sample preparation steps.

Merging conformational landscapes in a single consensus space with FlexConsensus algorithm

The paper introduces FlexConsensus, an algorithm that merges conformational landscapes from different CryoEM heterogeneity methods into a shared consensus space to improve reliability and reproducibility.

Mind the corner: Fillets in cryo-FIB lamella preparation to minimise sample loss caused by stress concentration and lamella breakage

The paper introduces a modification to cryo-FIB milling patterns by adding fillets to reduce stress concentration and lamella breakage, improving yield by 33%.

Dose-Efficient Cryo-Electron Microscopy for Thick Samples using Tilt-Corrected Scanning Transmission Electron Microscopy, Demonstrated on Cells and Single Particles

This paper introduces tilt-corrected bright-field scanning-TEM (tcBF-STEM) for cryo-EM, showing improved contrast and efficiency for thick samples, with a subnanometer resolution SPA map of a virus-like particle.

The CAGE complex: a hollow, megadalton, protein assembly in prokaryotic and eukaryotic microbes

The paper identifies and characterizes a new conserved protein complex, CAGE, found in both prokaryotes and eukaryotes, revealing its structural features and evolutionary distribution.

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-EM micrographs

CryoFSL improves protein particle picking in cryo-EM micrographs with minimal annotations, achieving high accuracy and efficiency in low-SNR conditions.

LieOTMap-FFT: A Differentiable Fitting Framework Combining Lie Algebra and FFT-accelerated Optimal Transport for Cryo-EM Maps

LieOTMap-FFT aligns cryo-EM maps with atomic models using Lie algebra and FFT-accelerated optimal transport, achieving high accuracy in rigid body fitting.

LieOTMap: A Differentiable Approach to Cryo-EM Fitting via Lie-Theoretic Optimal Transport

LieOTMap fits high-resolution atomic models into cryo-EM maps using a differentiable framework based on Lie algebra and optimal transport, achieving accurate alignment of E. coli GroEL.

Correlative MS Imaging for in situ cryo-ET

This paper introduces a combined MSI and cryo-ET pipeline for reliable single-cell classification in cryo-ET samples, enabling post-acquisition cell identification and classification.

LieMap: A Fast and Transparent Gradient-Based Method for Cryo-EM Structure Fitting

LieMap fits high-resolution atomic models into cryo-EM maps using gradient-based optimization, achieving a low RMSD for E. coli GroEL structure fitting.

RobPicker: A Meta Learning Framework for Robust Identification of Macromolecules in Cryo-Electron Tomograms

RobPicker improves macromolecule identification in cryo-ET by addressing label noise and class imbalance through a meta-learning framework, outperforming existing methods in detecting small and rare species.

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

This paper introduces a method to overcome preferred orientation in cryo-EM by using ultrasonic excitation during vitrification, improving data acquisition efficiency.

Continuous Serial Electron Diffraction for High Quality Protein Structures

This paper introduces c-SerialED, a method for high-quality protein structure determination using continuous serial electron diffraction, achieving atomic resolution with reduced radiation damage.

The separation of the effects of atomic displacement and local resolution in crystallographic and cryo-electron microscopy maps

The paper introduces a method to separate atomic displacement and local resolution effects in crystallographic and cryo-EM maps by modeling Fourier ripples.