Hedgehog Signaling Pathway Studies

Wunen(s) help navigate Primordial Germ Cells by attenuating Hedgehog signaling

The paper shows that Wunen(s) inhibit Hedgehog signaling in Drosophila PGCs by affecting Smo localization and lipid metabolism, preventing premature clumping.

AlphaFold3 predictions of novel GLI-SUFU interfaces identify binding-defective SUFU missense variants from medulloblastoma and Gorlin Syndrome patients

The paper uses AlphaFold3 to predict GLI-SUFU complex structures, identifies binding-defective SUFU variants from patients, and validates their functional impact.

In vitro reconstitution of vertebrate Sonic Hedgehog protein cholesterolysis

This study establishes an in vitro FRET-based assay to monitor cholesterolysis activity of vertebrate SHhC domains, revealing substrate specificity and identifying a conserved aspartate residue critical for activity.

Lipid moieties of sonic hedgehog are important for interaction with its inhibitor, WIF1

The paper shows that lipid modifications on sonic hedgehog (Shh) enhance its binding to WIF1, an inhibitor, suggesting lipid moieties are critical for their interaction.

Smoothened turnover regulated by Hedgehog signaling in Drosophila

The paper investigates protein turnover and intracellular trafficking of Hedgehog signaling components in Drosophila wing discs and finds that Smoothened's distribution and half-life are regulated by Hedgehog signaling.

Exosome secretion is required for sonic hedgehog dispersal and signal gradient formation in the embryonic limb mesenchyme

PTEN restrains SHH medulloblastma growth through cell autonomous and nonautonomous mechanisms

This study shows that PTEN loss accelerates SHH medulloblastoma growth through both cell-autonomous and nonautonomous mechanisms in mouse models.

Sharp cell-type boundaries emerge from temporal coordination between morphogen signals

The paper shows that coordinated timing between Wnt and Hedgehog signaling generates sharp cell-type boundaries during hair follicle development.

Patched regulates cell cycle and tissue architecture in C. elegans gonad

This study shows that Patched receptors in C. elegans regulate germ cell development and tissue architecture through cholesterol-sensitive mechanisms.

Direct cell-to-cell transport of Hedgehog morphogen is aided by the diffusible carrier Shifted/DmWif1

This study identifies Shf as a lipid-shielding protein that facilitates cell-to-cell transport of Hedgehog morphogen via cytonemes.

Mapping the temporal and functional landscape of Sonic Hedgehog signaling reveals new insights into early human forebrain development

This study maps the temporal and functional landscape of Sonic Hedgehog signaling in human forebrain development using stem cell models and identifies new markers of regionalization.

Smoothened inhibition of PKA at cilia transduces Hedgehog signals

The paper shows that SMO inhibits PKA at cilia to activate the HH signaling pathway, dependent on GRK2/3.

Sox9-dependent plasticity of esophageal progenitors is fine-tuned by cues from the microenvironment

The paper shows that Sox9-dependent plasticity in esophageal progenitors is regulated by Hedgehog-induced TGF-β and BMP pathways, and can be modulated by Ibuprofen in vivo.

Receptor Allostery Promotes Context-Specific Sonic Hedgehog Signaling During Embryonic Development

This study identifies an arachidonic acid binding pocket in SMO that modulates SHH signaling, revealing context-specific allostery crucial for cardiopulmonary development.

Physiological analysis of the mechanism of Ci transcription factor activation through multiple Fused phosphorylation sites in Hedgehog signal transduction

The paper investigates how phosphorylation sites on Ci regulate its activation in Drosophila through interactions with Fu kinase and Su(fu), revealing new insights into Ci conformation and activation mechanisms.

Proximity based proteomics reveals Git1 as a regulator of Smoothened signaling

The paper uses proximity proteomics to identify Git1 as a regulator of Smo signaling in the Hedgehog pathway, showing its role in Grk2 transport and Smo phosphorylation.

Hedgehog-Hippo pathway interactions promote T cell exclusion from the tumor microenvironment in basal cell carcinoma

This study shows that HH/GLI and Hippo/YAP pathway interactions suppress T cell chemotaxis and promote immunosuppression in BCC by downregulating chemokine signaling.

The novel linkage between Fuz and Gpr161 genes regulates sonic hedgehog signaling during mouse embryonic development.

This study identifies a genetic and biochemical link between Fuz and Gpr161 in regulating sonic hedgehog signaling during mouse embryonic development.

A dynamic Hedgehog gradient orients tracheal cartilage rings

The paper investigates how a dynamic Hedgehog gradient organizes tracheal cartilage rings into parallel stripes through a Turing-like mechanism.

A Scube2-Shh feedback loop links morphogen release and spread to morphogen signaling to enable scale invariant patterning of the ventral neural tube

The paper shows that Scube2 and Shh form a feedback loop that enables the Shh morphogen gradient to scale with tissue size in the ventral neural tube.

Growth plate resting zone chondrocytes acquire transient clonal competency upon Hedgehog activation and efficiently transform into trabecular bone osteoblasts

This study shows that Hedgehog activation in resting zone chondrocytes converts them into osteoblasts through clonal expansion and migration.

Iterative remodeling of the mouse uterus requires Hedgehog signaling

This study identifies Hedgehog signaling as essential for cyclical uterine remodeling in mice, showing that its disruption prevents remodeling and causes smooth muscle hypertrophy.

Frem1 activity regulated by Sonic Hedgehog signaling in the cranial neural crest mesenchyme guides midfacial morphogenesis

This study identifies Frem1 as a target of Sonic Hedgehog signaling in cranial neural crest cells, showing its role in midfacial morphogenesis and face shape variation.

Hedgehog pathway members Patched and Costal-2 exhibit differences in overgrowth autonomy in Drosophila melanogaster

The paper identifies differences in overgrowth autonomy between two Hedgehog pathway mutants in Drosophila, linking these differences to deregulation of the Mad downstream effector.

Non-cell autonomous inhibition of the Shh pathway due to impaired cholesterol biosynthesis requires Ptch1/2

The paper shows that mutations in cholesterol synthesis genes impair Shh signaling in neighboring cells via Ptch1/2 efflux of cholesterol precursors.

Glypicans specifically regulate Hedgehog signaling through their interaction with Ihog in cytonemes

This study investigates how glypicans regulate Hedgehog signaling by interacting with Ihog in cytonemes in Drosophila.

Identification of novel disease relevant genetic modifiers affecting the SHH pathway in the developing brain

The paper identifies Pttg1 and Ulk4 as genetic modifiers that enhance SHH pathway resilience and are involved in primary cilia formation in the neuroepithelium.

HES1 is a critical mediator of the SHH-GLI3 axis in regulating digit number

The paper identifies HES1 as a critical mediator of the SHH-GLI3 axis in regulating digit number, showing its role in inducing polydactyly and modulating cell proliferation and chondrogenesis.

Association of Sonic Hedgehog with the Extracellular Matrix Requires its Putative Zinc-Peptidase Activity

This study shows that Sonic Hedgehog's zinc-coordination fold is necessary for its association with the extracellular matrix and non-cell autonomous signaling.

GPR17 is an Essential Component of the Negative Feedback Loop of the Sonic Hedgehog Signalling Pathway in Neural Tube Development

This study identifies GPR17 as a negative regulator of the Sonic Hedgehog signaling pathway in neural tube development, showing its role in temporal regulation and feedback inhibition.