Pluripotent Stem Cells Research

Protracted fate acquisition and epigenetic de-aging during induced neural stem cell conversion of human blood cells

This study converts human blood cells into induced neural stem cells, showing prolonged epigenetic de-aging and delayed acquisition of neural stem cell identity over time.

An age-preserving neuronal iSpheroid platform for modeling human aging and Alzheimer disease

This paper introduces an age-preserving neuronal iSpheroid platform that models human aging and Alzheimer's disease, revealing disease-associated metabolic changes and potential therapeutic targets.

Engineering robust cardiomyocyte differentiation from iPSCs through WNT-mediated process window expansion

This study shows that early WNT inhibition expands the process window for robust cardiomyocyte differentiation from hiPSCs, improving efficiency and reducing variability.

Suspension culture drives metabolic rewiring in human pluripotent stem cells

This study compares 2D and 3D suspension cultures of human pluripotent stem cells, finding metabolic rewiring in 3D cultures with increased glycolysis and altered TCA cycle carbon partitioning.

ISP Platform powered by Geneformer: Framework for Cross-Species, State-feedback, and Multi-Gene In Silico Perturbation Screens with Application to iPS Cell State Transitions

Modeling human embryonic adrenogenesis in pluripotentstem cell-derived corticomedullar-like assembloids

This study develops a method to model human adrenal development using pluripotent stem cell-derived assembloids that recapitulate corticomedullar differentiation and function.

Decoding subtype development and function in human pluripotent stem cell-derived midbrain dopaminergic neurons

This study develops a method to derive and maintain specific subtypes of human midbrain dopaminergic neurons in vitro, revealing their functional and molecular characteristics and potential vulnerability in disease.

Microgels enable iPSCs to assemble, expand, and differentiate into organoids -- from sizable to high throughput

This paper introduces a method using PEG microgels to enable reproducible, scalable iPSC organoid production and differentiation into specific tissues.

Sustained 10% Oxygen Promotes Atrial Rather Than Ventricular Specification During Human iPSC-Cardiomyocyte Differentiation

In vitro programming and pseudounipolarization of human iPSC-derived sensory neurons

This study demonstrates the generation of functional human sensory neurons from iPSCs using NBI factors, showing their electrophysiological properties and disease relevance.

Differentiation protocol and maturation shape the axial identity and synaptic state in human iPSC-derived spinal motor neurons

This study compares two protocols for generating human iPSC-derived spinal motor neurons, finding that the extended protocol produces more mature, spinal-specific neurons with enhanced synaptic features.

Comparison of cell-cycle gene expression dynamics and mRNA kinetics across mouse and human pluripotent systems

This study compares cell-cycle gene expression dynamics and mRNA kinetics across mouse and human pluripotent systems, revealing species-specific differences in transcriptional complexity and oscillatory control.

High-throughput platforms for genetic perturbation screening using CRISPR/Cas9 in human iPSC-derived macrophages for drug discovery

The paper establishes a CRISPR/Cas9 screening platform in hiPSC-derived macrophages to identify genetic targets influencing inflammatory responses and functional phenotypes.

A Framework for NGN1-Induced Sensory Neuron Differentiation for Disease Modelling and Drug Screening

This paper develops a standardized framework for generating sensory neurons from human iPSCs using NGN1, improving differentiation efficiency and functional maturation for disease modeling and drug screening.

Polyclonal sensory neuron derivation from iPSCs as an efficient alternative to single clone strategies for pain relevant in vitro models

This paper develops polyclonal iPSC strategies to generate clonally diverse sensory neurons, reducing workload and enabling scalable drug-screening and patient-in-a-dish applications.

A panel of Diversity Outbred mouse embryonic stem cells: enabling the functional characterization of natural genetic variation in vitro

The paper describes a panel of genetically diverse embryonic stem cells from Diversity Outbred mice to study natural genetic variation's impact on pluripotency and differentiation.

Poor survival after myocardial infarction in immune deficient NXG B2m mice is alleviated by transplantation of induced pluripotent stem cell derived cardiomyocytes (iPSC-CM) formulated as spheres, but not single cells

This study shows that transplanting iPSC-derived cardiomyocytes as spheres improves survival in immune-deficient mice after MI compared to single cells, though sphere cells are less mature.

Nuclear βactin dependent chromatin accessibility governs stem cell pluripotency and extracellular matrix gene programs to maintain cellular biomechanics for cell lineage decisions

This study shows nuclear βactin regulates chromatin accessibility and ECM mechanics to maintain pluripotency and lineage decisions in mESCs.

Human iPSCs and iPSC-Derived Endothelial Cells from PAD Patients and Healthy Donors Exhibit Comparable Characteristics and Potency: Implications for Autologous Cell Therapy in Peripheral Artery Disease

This study compares hiPSCs and hiPSC-ECs from PAD patients and healthy donors, finding no significant differences in characteristics or therapeutic potency.

BCOR mutations establish a persistent culture-adaptive state in human induced pluripotent stem cells

This study identifies BCOR mutations in hiPSCs as a culture-adaptive state causing chromatin, transcriptomic, and proteomic changes, and develops a qPCR assay for detection.

Minimizing time in culture: A prototypic autologous manufacturing workflow for monoclonal iPSC lines within seven weeks

This study develops a time-efficient, standardizable workflow for generating monoclonal iPSC lines from human skin biopsies within seven weeks, with low variability and genomic integrity.

IF1 restrains excessive elevation of the mitochondrial membrane potential and safeguards the epithelial state of human iPSCs

This study shows that IF1 limits mitochondrial membrane potential elevation and prevents epithelial-mesenchymal transition in human iPSCs by inhibiting ATP hydrolysis.

Growth Factor-Based Manufacturing of Human Pluripotent Stem Cell-Derived Cardiomyocytes Using the Vertical Wheel Bioreactor System

This paper develops a scalable bioreactor-based method using growth factors to produce human pluripotent stem cell-derived cardiomyocytes with high purity and yield.

Clonal memory in human embryonic stem cells biases fate potential during endoderm differentiation

This study shows that clonal memory in human embryonic stem cells influences fate potential during endoderm differentiation by heritable chromatin states.

Navigating Human Astrocyte Differentiation: Direct and Rapid one-step Differentiation of Induced Pluripotent Stem Cells to Functional Astrocytes Supporting Neuronal Network development

This study presents a one-step protocol to differentiate hiPSCs into functional astrocytes, enabling co-culture with neurons to support network development.

Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs

This paper demonstrates that self-amplifying RNA (saRNA) enables durable, integration-free transgene expression in hiPSCs, supporting cell-fate programming and functional reporter analysis.

Generation and characterization of a patient-specific human induced pluripotent stem cell line from a Skogholt syndrome patient (ASCFi003-A)

This paper reports the generation and characterization of a patient-specific iPSC line from a Skogholt syndrome patient and its use to create choroid plexus organoids for disease study.

New iPSC resource with long-read whole genome sequencing characterizations for enhanced in vitro modeling

The paper introduces a new iPSC resource with long-read whole-genome sequencing data for in vitro modeling and genomic analysis.

Generation of effector CD4+ T cells from Human iPSC

This paper generates functional CD4+ T cells from human iPSC by modulating Notch signaling, showing cytokine diversity and transcriptional similarity to blood CD4+ T cells.

Transcriptomic Analysis Identifies Transient Mesendodermal State and Lineage Divergence in Human Pluripotent Stem Cell Differentiation