Pluripotent Stem Cells Research

Transcriptional landscape of direct reprogramming toward the hematopoietic lineage

The paper develops a single-cell transcriptomic reference atlas and evaluates a transcription factor recipe for reprogramming fibroblasts into hematopoietic stem cells, revealing intermediate transcriptomic states and transcriptional remodeling.

A Centimeter-Scale, Peristaltic Human Intestinal Organoid with Integrated Neuro-Immune-Vascular Systems Recapitulates Enteritis and Orthotopic Colorectal Cancer

This paper develops centimeter-scale human intestinal organoids with neuro-immune-vascular systems that model enteritis and colorectal cancer.

Impaired ZNF560 repression during induced pluripotent stem cell reprogramming indicates early epigenetic alterations in Schizophrenia

The paper identifies ZNF560 as an epigenetic marker in schizophrenia iPSCs, showing impaired repression and altered gene regulation during reprogramming.

Comparative methods for iPSC-Derived endothelial cells in modeling vascular diseases.

This study compares two media, APEL and BPEL, for generating iPSC-derived endothelial cells, finding they are equally effective but differ in cost and cell yield.

VEZF1 facilitates pluripotency exit by regulating developmental transcriptional programs and CTCF occupancy

Differentiation and maturation of iPSC-derived motor and sensory neurospheres using biomodified PEG-based microgels

This study examines the differentiation and maturation of motor and sensory neurospheres derived from iPSCs using bio-modified PEG microgels, showing subtype-specific responses and structural differences.

Rapid and efficient generation of human 8-cell-like cells for embryo modelling

This paper develops a method to rapidly generate human 8-cell-like cells (ri8CLCs) using small molecules, enabling efficient modeling of early embryogenesis.

Derivation of human post-mitotic cardiomyocytes from tetraploid iPSCs

This study generates tetraploid iPSCs to produce more mature human cardiomyocyte-like cells with improved characteristics compared to conventional iPSC-derived cardiomyocytes.

Vascularized Brain Organoid: A Versatile Platform Models Brain Cancer and Traumatic Brain Injury

This paper introduces a vascularized brain organoid platform to model brain cancer and traumatic brain injury.

TGF-β Signalling Drives Chemotaxis of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Response to MI Stimulus

This study shows hiPSC-CMs migrate toward MI tissue, driven by TGF-β signalling, and identifies TGF-β as a key mediator of this chemotactic response.

TGFβ-dependent upregulation of OCIAD2 is essential for epithelial-to-mesenchymal transition during mesendoderm differentiation

This study shows OCIAD2 upregulation during mesendoderm differentiation is TGFβ-dependent and essential for EMT, with implications for lineage commitment and tumor progression.

ERH elicits cell lineage restriction in mammalian preimplantation development and differentiation from pluripotency via H3K9me3-heterochromatin

This study shows ERH maintains H3K9me3 heterochromatin in mammalian cells, restricting lineage potential and repressing pluripotency genes during development and reprogramming.

Xeno-free human iPSC-derived prostate organoid platform for multilineage differentiation and genetic manipulation

This paper develops a xeno-free human iPSC-derived prostate organoid platform that enables multilineage differentiation and genetic manipulation for studying prostate biology and disease.

Rapid direct neuronal reprogramming of human dental pulp stem cells

This study reprograms human dental pulp stem cells into neurons more rapidly and efficiently than fibroblasts, using a lentiviral vector and single-nucleus RNA sequencing to characterize the process.

In Vivo Bioincubation Promotes Maturation of Human iPSC-Derived Cardiomyocytes in Neonatal Rat and Pig Hearts

This study shows that in vivo bioincubation of human iPSC-derived cardiomyocytes in neonatal rat and pig hearts promotes maturation to adult-like phenotypes with organized sarcomeres and mature morphology.

Modeling physiological insulin sensitivity and pre-diabetic insulin resistance in multiple hPSC-derived cell types

The paper develops a protocol to model insulin sensitivity and resistance in multiple hPSC-derived cell types, showing insulin resistance across various cell types under diabetic conditions.

Sustained epigenetic rejuvenation of serially engrafting human iPSC-derived HSCs

This study shows that iPSC-derived HSCs can maintain youthful epigenetic features through serial transplantation and differentiation.

Netrin-1 Acts as a Guardian of Naive Pluripotency in Human Embryonic Stem Cells

This study shows Netrin-1 supports naive pluripotency in hESCs by modulating epigenetic and signaling pathways, with effects dependent on culture conditions.

A Universal 6iL/E4 Culture System for Deriving and Maintaining Embryonic Stem Cells Across Mammalian Species

The paper develops a serum-free 6iL/E4 culture system that enables derivation and maintenance of embryonic stem cells (ESCs) across multiple mammalian species, revealing conserved mechanisms for ESC self-renewal.

Hepatic Differentiation of Human Pluripotent Stem Cells into Functional In Vitro Models Recapitulating Native Liver Complexity for MASLD Modelling

This paper develops a 10-day protocol to differentiate human pluripotent stem cells into hepatocyte-like cells and hepatic liver organoids, showing that organoids better model liver disease features than individual cells.

Isthmin-1 is a Key Regulator of Induced Pluripotent Stem Cell-Derived Cardiomyocytes Maturation through Activation of p53 Signaling

This paper identifies ISM1 as a key regulator of iPSC-CM maturation through p53 activation, improving structural and metabolic features.

Expanding genetic code to generate human brain organoids with both vasculature and microglia

This paper develops a method to generate human brain organoids with vasculature and microglia using genetic code expansion, showing enhanced neurovascular interactions and ZIKV-induced dysfunction.

Xeno-Free Peptide-Functionalized Hydrogels Support hiPSC Encapsulation and In Situ Differentiation into Cardiomyocytes with Enhanced Structural Maturation

This study develops a xeno-free hydrogel platform to support hiPSC expansion and in situ differentiation into structurally mature cardiomyocytes.

SALL2 constrains TEAD4 by maintaining repressive chromatin to restrict trophectoderm identity

This study shows SALL2 restricts trophectoderm identity by maintaining repressive chromatin at TEAD4-bound loci, preventing TE lineage commitment in human pluripotent stem cells.

Generation of spermatogonia from pluripotent stem cells in humans and non-human primates

This study generates spermatogonia from human and non-human primate pluripotent stem cells using xenogeneic testes, providing models for male infertility research.

Generation of three induced pluripotent stem cell lines from an immune checkpoint inhibitor-induced myocarditis patient and controls

The paper generates three iPSC lines from an ICIM patient and controls to study disease mechanisms and develop therapies.

Time-dependent BMP4 signaling directs lineage specification in human mesoderm

This study shows that timed BMP4 signaling directs mesoderm lineage specification in human pluripotent stem cells, with early BMP4 promoting lateral plate mesoderm and late BMP4 promoting intermediate mesoderm.

Mitochondrial activity directs nutrient uptake to control early human embryonic patterning

The paper shows mitochondrial activity regulates nutrient uptake, affecting BMP4 signaling and spatial organization of germ layers in human gastruloids.

Telomerase RNA regulates the epigenome primed for human lineage commitment

This study shows TERC regulates chromatin accessibility and epigenomic architecture in hESCs, essential for lineage commitment, independent of telomere length.

A PERTURBATION CELL ATLAS OF HUMAN INDUCED PLURIPOTENT STEM CELLS

The paper generates a CRISPRi perturbation cell atlas in human induced pluripotent stem cells, identifies novel regulators of pluripotency and metabolism, and proposes a machine learning framework for predicting perturbation recipes.