Nuclear Structure and Function

Pre-rRNA spatial distribution and functional organization of the nucleolus

This study maps the spatiotemporal distribution of pre-rRNA intermediates in the human nucleolus, revealing structural and functional differences between amniote and anamniote nucleoli.

Microcephaly-Associated Genes asp and Sas4 Control Chromatin Organization and Nuclear Lamina Structure in Drosophila melanogaster

This study shows that asp and Sas4 genes in Drosophila affect nuclear structure, chromatin organization, and genome stability, contributing to microcephaly pathogenesis.

The Deformability of the Mammalian Cell Nucleus is Determined by the Identity of the Lamin Rod Domain

This study identifies the A-type lamin rod domain as the key determinant of nuclear deformability, revealing its unique role in resisting large mechanical forces on the nucleus.

Sequence and structural rules define PY-NLS-dependent nuclear import by TRANSPORTIN 1 in plants

The paper identifies a plant-specific PY-NLS pathway mediated by TRN1, revealing structural features distinguishing plant PY-NLSs from animal and fungal models.

Disorder in coiled-coils orchestrate chromatin organization and define a new permeability barrier at the nuclear pore basket

The paper identifies disordered regions in the coiled-coil domain of TPR, showing they form condensates and create a permeability barrier at the nuclear pore basket.

Defects in lamin A-Prohibitin crosstalk leads to ROS elevation and OxPhos imbalance in laminocardiomyopathy

This study shows that the lamin A K97E mutation disrupts PHB2 interaction, causing mitochondrial dysfunction, ROS elevation, and metabolic imbalance, contributing to dilated cardiomyopathy.

Lamin B1 and LAP2β resist cytoskeletal force to maintain lamin A/C meshwork organization and preserve nuclear integrity

The paper shows that LB1 and LAP2β resist cytoskeletal forces to maintain lamin A/C meshwork and nuclear integrity, preventing rupture upon their depletion.

Structural basis for a nucleoporin exportin complex between RanBP2, SUMO1-RanGAP1, the E2 Ubc9, Crm1 and the Ran GTPase

The paper presents cryo-EM structures of a RanBP2 complex with Crm1, SUMO1-RanGAP1/Ubc9, and Ran(GTP), revealing interactions critical for mitotic progression.

A yeast model reveals the SMAD3 nuclear import mechanism

The paper uses a yeast model to identify the nuclear import mechanism of SMAD3, showing that the MH1 domain functions as an importin binding surface.

Mapping and engineering RNA-controlled architecture of the multiphase nucleolus

The paper maps rRNA processing dynamics in the nucleolus and shows how rRNA processing affects nucleolar phase organization and structure.

Visualizing developmental dynamics of nuclear morphology and transport machinery in Drosophila

The paper visualizes nuclear morphology and transport machinery in Drosophila during development, finding dynamic changes in NPC abundance and transport receptor puncta.

Advanced human iPSC-based modelling of LMNA-related congenital muscular dystrophy enables development of targeted genetic therapies for muscle laminopathies

This paper develops a human iPSC-based model of L-CMD to test gene editing strategies for muscle laminopathies, showing partial reversal of disease phenotypes with precise mutation correction.

Intrinsically Disordered Regions Form Nucleoli and Cajal Bodies While Fostering RNA Modification

The paper shows that Nopp140 concentrates intrinsically disordered regions to form the DFC, a phase-separated condensate that enables efficient rRNA modification.

The nucleus activates mechano-responsiveness via FHOD-associated LINC complexes

The paper shows the nucleus actively directs mechanical properties of actin cytoskeleton via FHOD-associated LINC complexes, and disruption causes cardiac hypertrophy.

The nucleus forms a dynamic contact with the plasma membrane to maintain the glandular epithelial architecture

This study identifies a dynamic nuclear-plasma membrane contact mediated by the LINC complex that maintains epithelial architecture through Src signaling.

Cardiomyopathic mutations of lamin A perturb mutual interactions of lamin, nuclear membrane, and chromatin leading to LLPS

This study shows that cardiomyopathic lamin A mutations disrupt nuclear structure and chromatin organisation, leading to liquid-like aggregates and genome spatial disorganisation.

Wetting and localization of chromatin condensates by surface-enriched proteins

The paper proposes a wetting-based mechanism for chromatin condensate localization at the nuclear lamina through surface-enriched proteins, showing nonmonotonic density profiles and regulatory effects of non-binding proteins.

The fastest movements and the most extreme deformations of the cell nucleus are driven by microtubules in the model fungus Podospora anserina

This study shows that microtubules drive rapid nuclear movement and extreme deformations in male nuclei of Podospora anserina during sexual reproduction.

In vivo nuclear envelope adaptation during cell migration across confining embryonic tissue environments

The paper investigates how neural crest cells adapt to physical confinement during migration in zebrafish embryos, finding that nuclear deformation occurs without DNA damage.

Visualizing nuclear pore complex plasticity with pan-Expansion Microscopy

This paper introduces pan-Expansion Microscopy to visualize nuclear pore complex plasticity, revealing LINC complex-dependent diameter biases and nucleoporin repositioning in an ALS model.

Conformational Dynamics of the Nuclear Pore Complex: Insights from Elastic Network Models and Coarse-Grained Simulations

This study uses elastic network models and molecular dynamics simulations to analyze the conformational dynamics of the nuclear pore complex, revealing mechanisms of its two-state transition and FG repeat behavior.

A protein-DNA surface hydrogel mechanically reinforces the cell nucleus and protects the genome

The paper shows a protein-DNA co-condensate forms a surface hydrogel that mechanically reinforces the nucleus and protects DNA from damage during mechanical stress.

Nuclear assembly in giant unilamellar vesicles encapsulating Xenopus egg extract

This study reconstitutes a nucleus in giant unilamellar vesicles using Xenopus egg extract, demonstrating functional nuclear import and pore complexes.

Rainbow Nucleus Charts Dynamic Interactome of Membrane-less Organelles

The paper constructs a Rainbow Nucleus cell line to study interactions between nuclear membrane-less organelles, finding structured, transcription-dependent contact networks.

A Conserved Disruption of the Nuclear Permeability Barrier in Meiosis is Controlled by a Kinase-Phosphatase Pair in Saccharomyces cerevisiae

The paper identifies a conserved meiotic mechanism called virtual nuclear envelope breakdown (vNEBD) in yeast, regulated by Ime2 kinase and Gip1 phosphatase.

Acute chromatin decompaction stiffens the nucleus as revealed by nanopillar-induced nuclear deformation in cells

The paper shows that acute chromatin decompaction increases nuclear stiffness in some cells but not others, depending on their ability to recompact chromatin.

Phase Behavior and Dissociation Kinetics of Lamins in a Polymer Model of Progeria

This study models lamin aggregation and dissociation in progeria using polymer chains to show how molecular interactions and concentration affect nuclear lamina structure and stability.

Lamin A/C Deficiency Drives Genomic Instability and Poor Survival in Small-Cell Lung Cancer through Increased R-loop Accumulation

This study shows that LMNA deficiency in SCLC increases R-loop accumulation, leading to genomic instability and poor patient survival.

Nuclear envelope budding is a non-canonical mechanism to export large transcripts in muscle cells

The paper demonstrates that nuclear envelope budding facilitates the export of large muscle-specific transcripts during myotube formation, involving UIF and ESCRT-III machinery.

Telomere Orchestrates Encapsulation of Mitotic Chromosomes

The paper shows telomeres initiate nuclear envelope reassembly during mitosis, ensuring proper chromosome encapsulation and preventing micronuclei formation.