Nuclear Structure and Function

Nuclear wrinkles result from geometric adaptations to cellular and nuclear morphological changes in epithelial cells

The paper investigates nuclear wrinkles in epithelial cells, finding they form due to geometric adaptations to mechanical stress and cell shape changes, without causing damage.

How the TREX-2 complex associates with the nuclear pore

The paper reports an extended structure of the human nuclear pore complex, revealing TREX-2 components as integral parts of the nuclear ring.

A novel FRET-force biosensor for nucleoporin gp210 reveals that the nuclear pore complex experiences mechanical tension

The paper develops a FRET-force biosensor to measure mechanical tension on the nuclear pore complex and finds that nuclear strain, not cytoskeletal forces, primarily drives these forces.

Emerin modulation impacts viability, proliferation, migration, and DNA repair signaling in cisplatin-treated glioblastoma cells

This study shows that modulating EMD levels in wild-type p53 GBM cells affects cisplatin response, viability, proliferation, and DNA repair signaling.

Spatial control of karyopherin binding avidity within NPC mimics revealed by designer FG-Nucleoporins

This study designs and tests artificial FG-Nups to show that spacing and charge-to-hydrophobicity ratio affect Kap95 binding and transport selectivity through NPCs.

In-cell structures visualize human pre-ribosome assembly in the nucleolus

This study visualizes human pre-ribosome assembly in nucleoli using cryogenic correlative light and electron tomography, revealing structural states and interactions.

Atypical RanGAP drives nucleocytoplasmic transport in a parasitic Alveolate

A RabGAP-fold protein, TBC9, supplies the missing RanGAP activity in Toxoplasma; its acidic C-terminal motif promotes Ran binding and marks TBC9 orthologs across non-ciliate alveolates.

Transcriptional activity generates chromatin motion that drives nuclear blebbing

The paper shows that transcriptional activity drives chromatin motion, which causes nuclear blebbing, independent of nuclear mechanics or actin antagonism.

A bipartite, mutation-tolerant NLS regulates interaction of ΔNp63α with importin alpha, nuclear transport and transcriptional activity

This study identifies a mutation-tolerant bipartite NLS in ΔNp63α that ensures nuclear import and transcriptional activity despite mutations.

Mutation-Agnostic Base Editing of the Progerin Farnesylation Site Rescues Hutchinson-Gilford Progeria Syndrome Phenotypes in Neuromuscular Organoids

This paper develops FATE, a mutation-agnostic base-editing platform that rescues HGPS phenotypes in neuromuscular organoids by disrupting the LMNA farnesylation motif.

Tom70-mediated mitochondria-nuclear envelope contacts regulate nuclear pore complex inheritance during gametogenesis

The paper identifies Tom70 as a regulator of nuclear pore complex exclusion during gametogenesis in yeast by mediating mitochondria-nuclear envelope contacts.

Components of an ESCRT-independent nuclear envelope assembly pathway

In S. japonicus, the ESCRT adaptor Alx1 activates the essential PH-domain protein Vid27 through interaction, driving postmitotic nuclear envelope sealing independently of Cmp7 and ESCRT-III.

Hydrophobic interactions of FG-nucleoporins are required for dilating nuclear membrane pores into selective transport channels after mitosis

The paper shows hydrophobic FG-nucleoporins dilate nuclear pores during mitosis, enabling nuclear import and NE reformation.

Nucleolar dynamics are determined by the ordered assembly of the ribosome

This study uses HiT-FRAP to show that ribosome assembly state affects nucleolar dynamics and morphology through interactions with NPM1.

Reconstitution of lamin assembly on nuclear pore complex-containing membranes

The paper demonstrates that lamin-B3 can assemble into filamentous structures in Xenopus laevis egg extracts under nucleoplasm-like conditions, independent of nuclear assembly.

Chromatin condensates tune nuclear mechano-sensing in Kabuki Syndrome by constraining cGAS activation

The paper shows that chromatin condensates, regulated by MLL4, modulate nuclear mechano-sensing and prevent cGAS-STING activation in Kabuki Syndrome.

The EGFR ligand amphiregulin regulates genomic integrity by facilitating heterochromatin formation in response to replication stress

This study shows that amphiregulin (AREG) in the nucleus helps maintain genomic stability by supporting heterochromatin formation during replication stress, especially in BRCA2 mutant cells.

The structural basis of RanGAP1 regulation and catalysis in nuclear transport

This study reveals the structural basis of RanGAP1 regulation and catalysis in nuclear transport, showing how sumoylation and conformational changes coordinate GTP hydrolysis.

Amphipathic helices sense the inner nuclear membrane environment through lipid packing defects

The paper identifies how amphipathic helices bind to the inner nuclear membrane by sensing lipid packing defects rather than electrostatics, showing the INM's loose packing under basal conditions.

A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments

The paper introduces a method to pulse-label yeast Nuclear Pore Complexes using a nanobody, enabling tracking in imaging and biochemical experiments.

Granular component sub-phases direct ribosome biogenesis in the nucleolus

The paper uses super-resolution microscopy to show that nucleolus granular component sub-phases are organized into multiphase condensates that facilitate ribosome subunit assembly.

A conserved mechanism of membrane fusion in nuclear pore complex assembly

This study identifies Brl1 and Brr6 as conserved fusogens mediating membrane fusion during NPC assembly in eukaryotes.

Mlp1 and Mlp2 cooperate to build a stoichiometric nuclear pore basket in budding yeast

The paper investigates the interactions required for nuclear basket formation in budding yeast and proposes a refined model with a 4:2:1 stoichiometry of Mlp1:Mlp2:Pml39 per spoke.

Progerin-Induced Nuclear Envelope Remodeling is Shaped by Cell Division and NUP153

This study shows progerin causes nuclear envelope remodeling in two stages, with NUP153 promoting invagination, and links these changes to aging-related nuclear morphology.

Polarization Increases Nuclear Stiffness in Macrophages Despite Reduction in Lamin A/C Levels

This study shows that pro-inflammatory polarization of macrophages increases nuclear stiffness despite reduced lamin A/C levels, suggesting chromatin changes drive nuclear mechanics.

Lamin B1 Safeguards the B Cell Genome and Shapes Lymphoma Outcome

This study shows that Lamin B1 protects B cell genomes by preventing DNA damage and regulating chromatin accessibility, with its loss linked to poor lymphoma outcomes.

Vimentin molecular linkages with nesprin-3 enhance nuclear deformation by cell geometric constraints

This study shows vimentin enhances nuclear deformation in polarized cells by linking to nesprin-3 and transmitting forces through the nuclear envelope.

The role of the nuclear pore complex in the stability of disease-related short tandem DNA repeats

This study shows disease-related short tandem repeats relocate to nuclear pore complexes, with implications for genome stability and repeat dynamics.

Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics

This study shows that A-type and B-type lamins have opposing roles in LBR localization and dynamics, with lamin A displacing LBR from the nuclear envelope while lamin B1/B2 tether it.

Sequence and Structural Alignments Reveal Insights into ANKLE2 Evolution and Function

The paper uses sequence and structural alignments to study ANKLE2 evolution and function, revealing conserved domains and potential roles in DNA damage response.