Lipid metabolism and biosynthesis

An optimized method to visualize lipid droplets in brain tissue demonstrates their substantial accumulation in aged brains

Polyketide Synthase-Like Functionality Acquired by Plant Fatty Acid Elongase

The paper identifies a novel fatty acid elongation pathway in Orychophragmus limprichtianus that produces keto-hydroxy fatty acids via a polyketide synthase-like mechanism.

Endoplasmic Reticulum Associated Lipolysis Regulates Hepatic Fat Synthesis and Turnover

This study identifies AADAC as a regulator of hepatic lipid metabolism, showing its role in suppressing lipid synthesis and preventing MASLD under overnutrition.

Nuclear Pct1 couples phosphatidylcholine synthesis with membrane biogenesis

The paper shows that Pct1, a nuclear enzyme in yeast, senses lipid imbalance and coordinates phosphatidylcholine synthesis with membrane biogenesis.

GPI lipid remodeling regulates lipophagy by forming lipid domains in response to glucose deprivation

This study shows that GPI lipid remodeling is essential for forming lipid domains that mediate lipophagy during glucose starvation in yeast.

Neuronal lipid droplets play a conserved and sex-biased role in maintaining whole-body energy homeostasis

The paper identifies neuronal lipid droplets as conserved structures that regulate energy homeostasis with sex-biased effects in flies and mice.

The reticulon homology domain of Pex30 generates membrane curvature at ER subdomains for lipid droplet biogenesis

The fatty acid synthesis pathway is a checkpoint for lipoteichoic acid synthesis in Staphylococcus aureus

This study identifies the fatty acid synthesis pathway as a key regulator of lipoteichoic acid synthesis in Staphylococcus aureus by controlling glycerophosphate availability.

Box H/ACA snoRNP regulates lipid storage through insulin signaling pathway in Drosophila melanogaster

The paper identifies GAR1 as a key regulator of lipid storage and insulin signaling in Drosophila, showing its role in alternative splicing and metabolic homeostasis.

Substrate-dependent oligomerization modulates DGAT1 activity and subcellular localization

This study investigates how substrate-dependent oligomerization affects DGAT1 activity and subcellular localization through molecular dynamics, biochemical reconstitutions, and live-cell fluorescence microscopy.

Cold acclimation reprograms hepatic lipid composition toward n-3 HUFAs to uncouple adipose-derived lipid flux from steatosis

The paper shows cold acclimation increases n-3 HUFAs in the liver, reducing steatosis by altering lipid composition and metabolism.

Lipid remodelling enables adaptation to chronic hyperosmotic stress

The paper shows that chronic hyperosmotic stress in budding yeast leads to lipid droplet accumulation and membrane remodelling, which are essential for cell fitness.

Oligomeric assemblies of plant biotin carboxylase revealed by cryo-EM and cross-linking

This study uses cryo-EM and cross-linking to reveal the oligomeric structure of plant biotin carboxylase, showing asymmetric dimer-of-dimers and dynamic B-domain interactions.

Spatially resolved rewiring of mitochondria-lipid droplet interactions in hepatic lipid homeostasis

Imaging of mitochondria and lipid droplets (LDs) across mouse liver lobules shows fasting, not Western diet, expands mitochondria-LD contacts; PLIN5 phosphomutants tune contacts and lipid storage.

Mitochondrial control of glycerolipid synthesis by a PEP shuttle

This study identifies SLC25A35 as a mitochondrial PEP transporter that regulates glycerolipid synthesis in lipogenic cells, with implications for metabolic diseases.

Origin of Eukaryotic Plasmalogen Biosynthesis by Horizontal Gene Transfer from Myxobacteria

The paper traces the origin of eukaryotic plasmalogen biosynthesis to horizontal gene transfer from Myxobacteria, revealing evolutionary divergence in plasmalogen synthesis across eukaryotic lineages.

Defensive lipid droplets are PUFA reservoirs driving bacterial clearance and inflammation

The paper shows that lipid droplets store polyunsaturated fatty acids (PUFAs) that are critical for bacterial clearance and inflammation by macrophages.

Diet- and metabolic state-dependent remodeling of the mouse brain lipidome

This study shows that the mouse brain lipidome changes with diet and metabolic state, particularly highlighting the impact of high-fat diets on lipid remodeling in the hypothalamus and brainstem.

Chemical Probing of Diacylglycerol Dynamics at Lipid Droplets

This paper develops a DAG-mimetic fluorescent probe, DONDI-5, to visualize diacylglycerol dynamics at lipid droplets and shows DAGs can be stored there without converting to triacylglycerols.

CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly

This study identifies CLCC1 as a regulator of hepatic neutral lipid flux and nuclear pore complex assembly through CRISPR screens and functional analyses.

CLCC1 Governs Bilayer Equilibration at the Endoplasmic Reticulum to Maintain Cellular and Systemic Lipid Homeostasis

This study identifies CLCC1 as a key regulator of phospholipid trans-bilayer equilibration at the ER, essential for lipid homeostasis and preventing metabolic diseases.

Stemona genomes illuminate fatty acid partitioning between seeds and elaiosomes mediating wasp dispersal

The paper generates chromosome-level genomes for two Stemona species to investigate fatty acid differentiation in elaiosomes and seeds, revealing molecular mechanisms underlying wasp seed dispersal.

Rab32/Rab38-positive Lysosome-Related Organelle degrades lipid droplet in hepatocytes by microautophagy

This study identifies Rab32/38-positive lysosome-related organelles as a platform for microautophagy-mediated lipid droplet degradation in hepatocytes.

Structural Basis of Substrate Selectivity and Catalysis in the Mycobacterial Long-Chain Acyl-CoA Carboxylase

This study determines the structure of the mycobacterial LCC complex to elucidate its substrate selectivity and catalytic mechanism.

Lipid Droplet Targeting Drives Substrate Access during Very Long Chain Fatty Acid Activation by Fat1

This study shows lipid droplets are the site of VLCFA activation by Fat1, with Fat1 targeting LDs via an amphipathic helix and using a hydrophobic cavity to access substrates.

Towards understanding the bacterial biosynthesis of branched GDGTs: Identification of iso-diabolic acid-based tetraester and mixed ether/ester, membrane-spanning lipid intermediates in members of the Bacillota

This study identifies brGDGT biosynthetic intermediates in Bacillota, revealing taxonomically dependent environmental regulation and proposing a biosynthetic pathway for brGDGT formation.

A Genome-wide Visual Screen Identifies Lysophosphatidylcholine as Counter Spatial Regulator of DAG and Sterols in Yeast

The paper identifies lysophosphatidylcholine (LysoPC) as a spatial regulator of DAG and sterols in yeast through a genome-wide screen and lipidomic analysis.

VAPB and its binding partner AKAP11 promote lipid droplet degradation

This study identifies VAPB and AKAP11 as regulators of lipid droplet homeostasis by mediating neutral lipid degradation through membrane contact sites and PKA activity.

Triglyceride/Cholesterol Ester Ratio Encodes Lipid Droplet Size and Diversity

The paper shows that the CE/TG ratio determines lipid droplet size and phase behavior, influencing their function and disease relevance.

Interorganelle competition for linoleic acid underlies steatotic liver pathology

This study identifies CLSTN3B as a driver of MASLD by promoting LD biogenesis and altering lipid partitioning between LDs and mitochondria, leading to mitochondrial dysfunction and oxidative stress.