Glycosylation and Glycoproteins Research

GlycoTraitR: an R package for characterizing structural heterogeneity in N-linked glycoproteomics data

GlycoTraitR is an R package that provides a unified framework for analyzing structural heterogeneity in N-linked glycoproteomics data through trait-based analysis.

The conserved NUS-1/DHDDS complex links N-glycosylation to lipid and lysosomal homeostasis in C. elegans

This study identifies the conserved NUS-1/DHDDS complex in C. elegans as essential for linking N-glycosylation to lipid and lysosomal homeostasis, revealing its role in preventing developmental failure and metabolic disorders.

A single catalytic domain residue regulates the substrate specificity of a mucin-type O-glycosyltransferase in salivary gland function

This study identifies a single amino acid difference in PGANT5 isoforms that affects substrate specificity and salivary gland function in Drosophila.

Identification of allo- or orthosteric VHH/single-domain antibodies that enhance or block pathogen binding to Siglec-1 on dendritic cells

The paper identifies VHH/single-domain antibodies that either block or enhance Siglec-1 ligand binding to sialylated pathogens on dendritic cells.

Nuclear N-Glycosylation Redefines the Glycoscape and Directs Cell Identity

The paper identifies nuclear N-glycosylation as a novel modification that influences cell identity and is linked to neurodevelopmental disorders.

GlycanGT: A Foundation Model for Glycan Graphs with Pretrained Representation and Generative Learning

GlycanGT is a foundation model for glycans that uses a graph transformer to improve classification and prediction of glycan structures and properties.

Glycolysis and hexosamine biosynthesis pathways are key for inflammatory protein maturation and leukocyte adhesion to human aortic valve cells

This study shows glycolysis and the hexosamine biosynthesis pathway are essential for inflammatory protein maturation and leukocyte adhesion in aortic valve cells during calcific aortic valve disease.

HIV-Induced Sialoglycans on Infected Cells Promote Immune Evasion from Myeloid Cell-Mediated Killing

This study shows HIV increases sialoglycan expression to engage Siglecs on myeloid cells, promoting immune evasion, and demonstrates that disrupting these interactions enhances immune killing of infected cells.

Sialoglycans on human T cells attenuate death programs executed through the Fas pathway

This study shows that sialoglycans on human T cells reduce Fas receptor-mediated apoptosis but not other TNF receptor pathways.

Detection of Cardiac O-GlcNAcylation via Subcellular Fractionation and Dual Antibody Analysis in Pressure Overload Cardiac Hypertrophy

This study detects changes in cardiac O-GlcNAcylation during pressure overload hypertrophy using subcellular fractionation and dual antibody analysis, revealing sex-specific and temporal patterns.

Discovering Glycosylation-Dependent Protein Function by Thermal Proteome Profiling

The paper uses thermal proteome profiling to study how glycosylation affects protein function and stability, finding that sialylation and fucosylation perturbations significantly impact cell surface protein interactions.

Atom-level Machine Learning of Protein-glycan Interactions and Cross-chiral Recognition in Glycobiology

This paper develops MCNet, a machine learning model that predicts protein-glycan binding strength using atom-level representations, enabling cross-chiral recognition and unifying diverse glycobiology datasets.

GlycoGenius: the ultimate high-throughput glycan composition identification tool

GlycoGenius automates glycan analysis from mass spectrometry data, reducing manual effort and processing time while identifying more glycans, including novel ones.

Anion supercharging enables structural assignment of oxidatively released O-glycans

This paper introduces a supercharging method using HFIP and butylamine to improve mass spectrometric analysis of oxidatively released O-glycans, enabling accurate structural assignment and discrimination of isomeric compositions.

Airway macrophage specific expression of a dense glycocalyx and its remodelling following viral infection

This study shows that airway macrophages have a glycocalyx that changes during viral infection, affecting their inflammatory response and repair.

Identification of sialic acid linkage profiles in primary liver cancers

This study identifies sialic acid linkage profiles in liver cancers to differentiate between cholangiocarcinoma subtypes and hepatocellular carcinoma.

RNA-Associated Glycoconjugates Highlight Potential Ambiguities in glycoRNA Analysis

The paper identifies potential ambiguities in glycoRNA analysis by showing that RNase sensitivity of glycosylated RNA species depends on purification methods, suggesting co-purification of non-glycoRNA glycoconjugates.

Proteins are a source of glycans found in preparations of glycoRNA

The paper shows that glycoproteins co-purify with RNA during glycoRNA isolation, suggesting they contribute glycans to glycoRNA preparations.

An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function

The paper develops Siglec-liposomes to detect Siglec ligands and modulate immune cell function, showing they enhance T cell proliferation.

Siglec-engaging immunosuppressive sialoglycans are upregulated in prostate cancer and are targetable to suppress bone metastasis

This study identifies Siglec-engaging sialoglycans as upregulated in prostate cancer and shows they can be targeted to suppress bone metastasis using a bisialidase.

Sialoglycans Modulate Siglec-5 - TLR4 Interactions in Osteoarthritis

The paper investigates how sialoglycans modulate Siglec-5-TLR4 interactions in osteoarthritis, showing sialylation patterns influence inflammation and joint integrity.

Glyco-STORM: Nanoscale Mapping of the Cellular Glycosylation Landscape

Glyco-STORM maps nanoscale glycosylation patterns in cells, revealing subcellular glycan domains and a spatial glycosylation code.

Patient-derived organoids to study glycosylation dynamics during gastric disease

This study uses patient-derived organoids to analyze glycosylation changes in gastric tissues and shows they mimic in vivo glycosylation profiles and H. pylori interactions.

Sialidases derived from Gardnerella vaginalis and Prevotella Timonensis remodel the sperm glycocalyx and impair sperm function

This study shows that sialidases from BV-associated bacteria damage the sperm glycocalyx, increasing susceptibility to agglutination and impairing cervical mucus transit.

Decoding the Glycan Signature: Unraveling N-Glycosylation Alterations in Glycogen Storage Disease Ia and Ib

The paper profiles serum N-glycans in GSD Ia and Ib patients and controls, identifying subtype-specific glycomic alterations and potential biomarkers for disease subtyping and tumor surveillance.

O-GlcNAc clusters attenuate BRD4 phase separation for transcription regulation

This study shows O-GlcNAc clusters modulate BRD4 phase separation, affecting transcription by altering condensate properties and gene expression programs.

Cancer-specific sialylation of insulin-like growth factor 1 receptor impairs therapeutic antibody binding and efficacy

This paper shows that cancer-specific sialylation of IGF1R reduces ganitumab binding and efficacy, and that desialylation enhances therapeutic response.

Time-Resolved Tracking and Secretome Mapping of Intracellular, Cell Surface, and Extracellular Glycoconjugates with a Mini-Tetrazine N-Acetylglucosamine

This paper introduces HTz-GlcNAc, a tool for real-time tracking of glycoconjugates and secretome mapping in live cells.

Lectin Microarray-based Glycomics and Machine Learning Identify Shared Osteoarthritis Biomarkers in Humans, Dogs, and Horses

This study uses lectin microarrays and machine learning to identify conserved and species-specific glycan signatures in synovial fluid that distinguish healthy from osteoarthritic joints in humans, dogs, and horses.

Quantitative analysis of inhibitor-induced assembly disruption in human UDP-GlcNAc 2-epimerase using mass photometry

The paper uses mass photometry to analyze how small-molecule inhibitors disrupt GNE oligomerization, finding concentration-dependent destabilization and competitive binding.