Cytomegalovirus and herpesvirus research

Apparent cooperativity between human CMV virions introduces errors in conventional methods of calculating multiplicity of infection

The paper investigates apparent cooperativity between human CMV virions and shows it affects multiplicity of infection calculations.

Inhibition of MAPK signaling suppresses cytomegalovirus reactivation in CD34+ Kasumi-3 cells

This study shows that inhibiting MAPK signaling suppresses CMV reactivation in CD34+ Kasumi-3 cells, suggesting a therapeutic target for preventing viral reactivation in immunocompromised patients.

MHC Class I-like Gene in Squirrel Monkey Cytomegalovirus and the Evolution of the Virus

The paper identifies a viral protein with high homology to human MHC class I molecules and traces its evolutionary origin through horizontal gene transfer.

Human cytomegalovirus gH/gL/gO binding to PDGFRα provides a regulatory signal activating the fusion protein gB that can be blocked by neutralizing antibodies.

This paper develops a cell-cell fusion assay to study HCMV gH/gL/gO receptor engagement and its role in activating gB fusion, showing that PDGFR binding enhances fusion and can be blocked by neutralizing antibodies.

Molecular cloning and host range analysis of three cytomegaloviruses from Mastomys natalensis

The paper clones and analyzes three cytomegaloviruses from Mastomys natalensis, showing their narrow host range and identifying a site for vaccine antigen insertion.

Human Cytomegalovirus Infection Reduces an Endogenous Antiviral Fatty Acid by Promoting Host Metabolism

This study shows HCMV infection reduces erucic acid levels, an antiviral fatty acid, by promoting metabolism that increases other fatty acids, thereby evading its antiviral effect.

Virus-against-virus dominant-negative interference strategy targeting a viral CC chemokine prevents cytomegalovirus-related neurodevelopmental pathogenesis

This study shows that a dominant-negative mutant of the viral chemokine RCK3 prevents CMV-induced neurodevelopmental disorders in a rat model.

A nonhuman primate model mirrors human congenital cytomegalovirus infection and reveals a spectrum of vertical transmission outcomes

The nonhuman primate model mirrors the epidemiology of human congenital CMV (cCMV) after primary infection and reveals its transmission bottlenecks.

Complex roles for proliferating cell nuclear antigen in human cytomegalovirus replication

This study investigates the role of PCNA in HCMV replication, finding it restricts virus replication and contributes to genome diversity through separable PCNA-dependent and -independent mechanisms.

Viral microRNA regulation of Akt is necessary for reactivation of Human Cytomegalovirus from latency in CD34+ hematopoietic progenitor cells and humanized mice

This study shows that HCMV miRNAs downregulate Akt to promote reactivation from latency in CD34+ cells and humanized mice.

The effective multiplicity of infection for HCMV depends on activity of the cellular 20S proteasome

This study shows that proteasome inhibition reduces HCMV replication by lowering the effective multiplicity of infection, and synergizes with antiviral drugs.

E2F3-dependent activation of FAM111B restricts mouse cytomegalovirus replication in primate cells

This study identifies FAM111B as a host restriction factor that suppresses mouse cytomegalovirus replication in human and rhesus macaque cells through E2F3-dependent activation.

CD4+ but not CD8+ T cells are required for protection against severe guinea pig cytomegalovirus infections

This study shows CD4+ T cells are critical for preventing severe congenital cytomegalovirus infection in guinea pigs, while CD8+ T cell depletion increases placental viral load without affecting disease severity.

Targeting the host transcription factor HSF1 prevents human cytomegalovirus replication in vitro and in vivo

Inhibiting HSF1 reduces HCMV replication in vitro and in vivo by targeting an early stage of the viral replication cycle.

Structure-based design of a soluble human cytomegalovirus glycoprotein B antigen stabilized in a prefusion-like conformation

This paper describes the stabilization of HCMV gB in a prefusion-like conformation using structure-based design and shows similar neutralizing antibody titers against postfusion and prefusion forms.

Stabilizing microtubules aids neurite structure and disrupts syncytia formation in human cytomegalovirus-infected human forebrain neurons

This paper investigates how HCMV infection affects microtubule networks in human forebrain neurons and shows that stabilizing microtubules reduces syncytia formation and neurite retraction.

The Epithelial-to-Mesenchymal Transition Supports HCMV Infection and Biosynthesis in Mammary Epithelial Cells Through Two Distinct Mechanisms.

This study shows that inducing epithelial-to-mesenchymal transition in mammary epithelial cells restores HCMV biosynthesis through two distinct mechanisms: enhanced viral entry and enhanced mRNA translation.

Cytomegalovirus infection in newborn mice alters cerebellar development by lengthening G1/S phases of cerebellar granule cell precursors during postnatal cerebellar development

This study shows that MCMV infection in newborn mice causes prolonged G1/S phases in cerebellar granule cell precursors, leading to reduced cerebellar size.

A UL26-PIAS1 complex antagonizes anti-viral gene expression during Human Cytomegalovirus infection

This study identifies the UL26-PIAS1 complex as a mechanism by which HCMV inhibits anti-viral gene expression and promotes cell-to-cell spread.

Highly Multiplexed Proteomic Analysis of HCMV-Infected Dendritic Cells Reveals Global Manipulation of Adaptive Immunity and Host Restriction of Viral Replication

The paper uses proteomic analysis to study HCMV-infected dendritic cells, revealing how the virus manipulates adaptive immunity and restricts replication.

Intermittent Cytomegalovirus Infection Alters Neurobiological Metabolism and Induces Cognitive Deficits in Mice

This study shows that intermittent CMV infection in mice alters BBB metabolism and causes cognitive deficits, suggesting a potential role for pathogens in dementia progression.

Human Cytomegalovirus Induces Significant Structural and Functional Changes in Terminally Differentiated Human Cortical Neurons

This study shows that HCMV can infect and replicate in terminally differentiated human cortical neurons, causing structural and functional changes including altered calcium signaling and neurotransmission.

Neutralizing Antibodies with Neurotropic Factor Treatment Maintain Neurodevelopmental Gene Expression Upon Exposure to Human Cytomegalovirus

The paper investigates how the source of HCMV affects neuronal pathogenesis in cerebral organoids and shows that neutralizing antibodies and neurotropic factors can mitigate gene expression dysregulation.

Persistent low-level variants in a subset of HCMV genes are highly predictive of poor outcome in immunocompromised patients with cytomegalovirus infection

The paper identifies a viral signature from HCMV genes associated with poor outcomes in immunocompromised patients, using deep sequencing and machine learning.

Transmission Dynamics of Human Herpesviruses and Other Blood DNA Viruses from Whole Genome Sequences of Families

The paper analyzes whole genome sequences of families to study the human blood DNA virome, finding evidence of household transmission and de novo viral integration.

Long Range PCR-based deep sequencing for haplotype determination in mixed HCMV infections

This paper introduces a long amplicon sequencing method to accurately determine haplotypes in mixed HCMV infections, showing improved resolution compared to short-read sequencing.

Functional single-cell genomics of human cytomegalovirus infection

This study uses CRISPR screening and single-cell transcriptomics to analyze the functional roles of host and viral factors in HCMV infection, revealing distinct impacts on infection trajectories.

Variation in Human Herpesvirus 6B telomeric integration, excision and transmission between tissues and individuals

The paper investigates the integration, excision, and transmission of HHV-6B in telomeres across tissues and individuals, revealing maternal transmission and dynamic transitions between integrated and free virus forms.

The zinc finger antiviral protein ZAP destabilises viral transcripts and restricts cytomegalovirus

This study shows that ZAP restricts HCMV infection by destabilizing viral transcripts with low CG content, revealing its role in antiviral defense against DNA viruses.

Mixed cytomegalovirus genotypes in HIV positive mothers show compartmentalization and distinct patterns of transmission to infants.

The paper shows that HIV-positive mothers have compartmentalized CMV genotypes, with three infants transmitting a single closely related maternal cervix strain, and identifies gene similarities linked to tissue-tropism and immunomodulation.