Nuclear Structure and Function

Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and to promote Dbp5 mediated tRNA export in vivo.

This study shows Dbp5 requires Gle1 to activate its ATPase activity for tRNA export and functions parallel to Los1 in tRNA transport.

A CDC-42-regulated actin network is necessary for nuclear migration through constricted spaces in C. elegans

The paper identifies CDC-42 and the Arp2/3 complex as regulators of nuclear migration in C. elegans P-cells, functioning parallel to the LINC complex.

Co-translational binding of importins to nascent proteins

The paper identifies importins that bind nascent proteins co-translationally in yeast, showing their role in preventing aggregation and integrating with ribosome-associated chaperones.

Differential contributions of nuclear lamina association and genome compartmentalization to gene regulation

The paper examines how nuclear lamina association and genome compartmentalization influence gene regulation across different cell types and treatments.

Specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport

This study investigates how 1,6-hexanediol disrupts nuclear pore complex permeability in yeast by displacing nuclear transport receptors without affecting NPC subunits or cell viability.

SIR telomere silencing depends on nuclear envelope lipids and modulates sensitivity to a lysolipid drug

This study shows that lysolipid treatment alters NE lipid composition, reducing Sir4 telomere association and triggering lipid-specific transcriptional changes in yeast.

The nucleolar aberrancies that drive ribosome impairment induced by RNA binding proteins are hallmarks of aging

This study identifies Hnrnpk as a master regulator of nucleolar stress and ribosome impairment, linking nucleolar abnormalities to aging and bone marrow failure.

Lamin A/C phosphorylation at serine 22 is a conserved heat shock response to regulate nuclear adaptation during stress

This study identifies lamin A/C phosphorylation at Ser22 as a conserved heat shock response that regulates nuclear structure and cell survival during stress.

An atlas of lamina-associated chromatin across twelve human cell types revealsan intermediate chromatin subtype

The paper maps lamin-associated chromatin across twelve human cell types, identifying two subtypes with distinct features and developmental dynamics.

Transcriptional profiling of Hutchinson-Gilford Progeria syndrome fibroblasts reveals deficits in mesenchymal stem cell commitment to differentiation related to early events in endochondral ossification.

This study identifies transcriptional deficits in HGPS fibroblasts related to mesenchymal differentiation and endochondral ossification, with age-dependent gene expression changes.

Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources

The paper measures nuclear transport rates in single yeast cells using FRAP and identifies variability in nuclear pore complexes and other factors as sources of cell-to-cell differences.

rRNA transcription is integral to liquid-liquid phase separation and maintenance of nucleolar structure

The paper shows that rRNA transcription by Pol I is essential for nucleolar structure and liquid-liquid phase separation in mouse embryos.

Human Nucleolar Protein 7 (NOL7) is required for pre-rRNA transcription and pre-18S rRNA processing

This study identifies NOL7 as the human ortholog of yeast Bud21, showing its essential role in pre-rRNA transcription and pre-18S rRNA processing, with consequences for protein synthesis and cell cycle progression.

Nucleoporins facilitate ORC loading onto chromatin

The paper shows that nucleoporins, specifically Elys, are required for ORC2 loading onto chromatin and proper replication initiation in Drosophila.

The molecular chaperone DNAJB6 provides surveillance of FG-Nups and is required for interphase nuclear pore complex biogenesis

This study identifies DNAJB6 as a molecular chaperone that prevents FG-Nup aggregation during interphase nuclear pore complex biogenesis.

Differential nuclear import sets the timing of protein access to the embryonic genome

The paper identifies that differential nuclear import timing controls gene expression onset in early embryonic development by regulating protein access to the genome.

Structure of Importin-4 bound to the H3-H4·ASF1 histone·histone chaperone complex

This study determines the structure of Importin-4 bound to the H3-H4·ASF1 complex, revealing how Importin-4 recognizes and transports the histone chaperone complex into the nucleus.

Nucleolar targeting in an early-branching eukaryote suggests a general physicochemical mechanism for ribosome protein sorting

The paper shows nucleolar targeting in Trypanosoma brucei is mediated by positive charge, contributing to sorting of mitochondrial and cytoplasmic ribosome proteins.

CTCF and cohesin promote focal detachment of DNA from the nuclear lamina

The paper investigates the role of CTCF and cohesin in LAD positioning, finding they reinforce borders but do not position LADs, and mediate fine-tuning of nuclear lamina interactions.

Stable twin rDNA loci form a single nucleolus in brewer's yeast

This study shows that twin rDNA loci in brewer's yeast form a single nucleolus and reveals position-dependent size changes in rDNA loci.

Artificial intelligence reveals nuclear pore complexity

The paper uses AI to create a dynamic model of the human nuclear pore complex, showing how the protein scaffold interacts with the nuclear envelope.

Impaired Lef1 activation accelerates iPSC-derived keratinocytes differentiation in Progeria

This study shows that impaired Lef1 activation in HGPS iPSCs accelerates keratinocyte differentiation, and base editing partially rescues this phenotype.

RNAP II produces capped 18S and 25S ribosomal RNAs resistant to 5-monophosphate dependent processive 5-3 exonuclease in polymerase switched Saccharomyces cerevisiae

The paper shows that in Saccharomyces cerevisiae, RNAP II produces capped 18S and 25S rRNAs resistant to 5-monophosphate dependent exonuclease, indicating a new role for RNAP II in ribosome biogenesis.

Mis-regulation of the Nucleoporins 98 and 96 lead to defects in protein synthesis that promote hallmarks of tumorigenesis.

This study shows that reducing Nup98-96 function in Drosophila leads to defects in protein synthesis and JNK signaling, promoting tumorigenesis when apoptosis is inhibited.

8 A structure of the nuclear ring of the Xenopus laevis nuclear pore complex solved by cryo-EM and AI

The paper determines the 8 Å cryo-EM structure of the Xenopus laevis nuclear pore complex nuclear ring using AlphaFold2 to build a pseudoatomic model.

Determining the architecture of nuclear ring of Xenopus laevis nuclear pore complex using integrated approaches

The paper determines the architecture of the nuclear ring in Xenopus laevis NPC using cryo-EM, homology modeling, and cell experiments, identifying key proteins and their roles in NPC assembly.

8 A structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex solved by cryo-EM and AI

The paper solves the 8 Å cryo-EM structure of the Xenopus laevis nuclear pore complex cytoplasmic ring using AlphaFold2, revealing detailed structural features of key components.

A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription

The paper shows that the Nup84 complex restricts Ty retrotransposon transcription in yeast by tethering Ulp1 to NPCs, preventing retrotransposition.

The Nup2 meiotic-autonomous region relieves autoinhibition of Nup60 to promote progression of meiosis and sporulation in Saccharomyces cerevisiae

The paper investigates the role of Nup2 and Nup60 in meiosis and sporulation in yeast, showing Nup60's primary role in chromosome recruitment and Nup2's secondary role in counteracting Nup60 autoinhibition.

Phosphorylation of luminal region of the SUN-domain protein Mps3 promotes nuclear envelope localization during meiosis

This study identifies phosphorylation of Mps3's luminal region as critical for its nuclear envelope localization during meiosis, mediated by CDK and DDK.