Nuclear Structure and Function

Human spermatogenesis leads to a reduced nuclear pore structure and function

This study investigates the structural and functional changes in human sperm nuclear pore complexes (NPCs) during spermatogenesis, revealing a reduced central channel and altered transport dynamics.

Continuous nucleolar ribosomal RNA synthesis in differentiating lens fiber cells until abrupt nuclear degradation required for ocular lens transparency

The paper shows that rRNA transcription continues in differentiating lens fiber cells until nuclear degradation, supporting translational mechanisms for crystallin protein production.

Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cells

This study shows WRN helicase maintains constitutive heterochromatin in human fibroblasts by interacting with HDAC2 and HP1, and its loss disrupts heterochromatin structure and LBR expression, contributing to aging and senescence.

Nucleoporin Nup153 docks the splicing machinery to the nuclear pore for efficient mRNA processing

This study identifies Nup153 as a docking point for splicing machinery at the nuclear pore, showing its role in mRNA processing and gene splicing.

Nuclear Type I Myosins are Essential for Life and Genome Organization

This study shows that nuclear type I myosins are essential for yeast cell viability and genome organization, with their depletion causing 3D genome disorganization and nuclear structural defects.

Expansion in situ genome sequencing links nuclear abnormalities to hotspots of aberrant euchromatin repression

The paper introduces ExIGS, a method linking nuclear abnormalities to chromatin repression hotspots in progeria fibroblasts.

Structural insight into the nuclear transportation mechanism of PPARg by Transportin-1

The paper determines the Cryo-EM structure of PPARγ in complex with Transportin-1, revealing a non-canonical NLS recognition mechanism.

Identification of nuclear pore proteins at plasmodesmata: potential role in intercellular transport?

The paper identifies FG-nucleoporins (FG-NUPs) at plasmodesmata (PD) using bioinformatics, proteomics, and imaging, suggesting a potential role in intercellular transport.

Reconstruction of artificial nuclei with nuclear import activity in living mouse oocytes

The paper reconstructs artificial nuclei with nuclear import activity in living mouse oocytes using T4 phage DNA, showing they can form functional nuclear structures similar to natural nuclei.

The tubule-sheet continuum model links the pattern of nuclear envelope assembly to nuclear envelope integrity

The paper shows that cancer cells use a NE assembly pattern linked to fragility, differing from normal cells.

Nucleoporin Elys attaches peripheral chromatin to the nuclear pores in interphase nuclei

The paper identifies Elys as a nucleoporin linking peripheral chromatin to nuclear pores in interphase nuclei and shows its role in chromatin organization and gene regulation.

Coaching ribosome biogenesis from the nuclear periphery

This study shows that nuclear invaginations regulate ribosome biogenesis by contacting nucleoli, with high curvature increasing ribosome production and low curvature reducing it.

Characterizing interactions in the nuclear pore complex transporter using novel site-specific deuteration and SANS

The paper uses site-specific deuteration and SANS to study the dynamic structure of FG Nups interacting with NTF2, revealing their fully disordered nature and lack of significant inter-aromatic contacts.

The Effects of Lamin B Receptor knockdown on a Myeloid Leukemia Cell

This study investigates the effects of Lamin B Receptor knockdown on the transcriptome and morphology of HL-60/S4 cells, identifying changes in Golgi and ribosome-related genes and nucleolar structure.

Nuclear envelope rupture in cardiomyocytes orchestrates early transcriptomic changes and immune activation in LMNA-related dilated cardiomyopathy that are reversed by LINC complex disruption

This study identifies NE rupture in LMNA-DCM cardiomyocytes as a driver of transcriptomic changes and immune activation, which can be reversed by LINC complex disruption.

Pervasive nuclear envelope ruptures precede ECM signaling and disease onset without activating cGAS-STING in Lamin-cardiomyopathy mice

The paper shows that NE ruptures occur before ECM signaling and disease in Lamin-cardiomyopathy mice without activating cGAS-STING.

Anchorage of H3K9-methylated heterochromatin to the nuclear periphery helps mediate P-cell nuclear migration though constricted spaces in Caenorhabditis elegans

The paper shows that anchoring H3K9-methylated heterochromatin to the nuclear periphery is necessary for P-cell nuclear migration through constrictions in C. elegans, independent of the LINC complex.

Parallel genetic screens identify nuclear envelope homeostasis as a key determinant of telomere entanglement resolution in fission yeast

The paper identifies nuclear envelope homeostasis as critical for resolving telomere entanglements in fission yeast through genetic screens and functional analysis.

DNA double-strand break-capturing nuclear envelope tubules drive DNA repair

The paper shows that DNA double-strand breaks are repaired by nuclear envelope tubules formed through cytoplasmic microtubule interactions, enhancing repair accuracy and cancer therapy efficacy.

Beyond microtubules: The cellular environment at the endoplasmic reticulum attracts proteins to the nucleus, enabling nuclear transport

The paper shows that proteins interact with the endoplasmic reticulum to accumulate near the nucleus without energy expenditure, following Chapmans law instead of Ficks law.

Genetically Encoded FRET Biosensor for Live-cell Visualization of Lamin A Phosphorylation at Serine 22

This paper develops a FRET-based biosensor to visualize lamin A phosphorylation at Serine 22 in live cells, revealing its dynamics during the cell cycle and its role in nuclear envelope breakdown.

Exportin-1 functions as an adaptor for transcription factor-mediated docking of chromatin at the nuclear pore complex.

The paper identifies Crm1 as an adaptor that links transcription factors to chromatin at the nuclear pore complex, facilitating enhancer docking.

Nucleolar reorganization after cellular stress is orchestrated by SMN shuttling between nuclear compartments

The paper shows that SMN shuttles from Cajal bodies to the nucleolus during DNA repair, aiding in nucleolar structure restoration after genotoxic stress.

Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis

The paper shows that genome organization influences NPC formation and differentiation in Drosophila oogenesis by regulating gene silencing and Nup activation.

Rapid meiotic prophase chromosome movements in Arabidopsis thaliana are linked to essential reorganization at the nuclear envelope

This study shows that Arabidopsis thaliana meiotic centromeres undergo rapid movements during zygotene and pachytene stages, which depend on SUN-domain proteins and are disrupted in sun1 sun2 mutants.

Evolutionary conserved protein motifs drive attachment of the plant nucleoskeleton at nuclear pores

The paper identifies conserved protein motifs in KAKU4 and related nucleoporins that mediate nucleoskeleton attachment to nuclear pores in plants.

Nuclear Lipid Droplets Exit to the Cytoplasm: A Novel Mechanism of non-nuclear pore-dependent material exchange

This paper identifies a non-nuclear pore-dependent mechanism for nuclear lipid droplet (nLD) efflux into the cytoplasm through lamina depolymerization and RhoA-PKC signaling.

Nuclear translocation of LINE-1 ORF1p alters nuclear envelope integrity and disrupts nucleocytoplasmic transport in human neurons

This study shows that ORF1p from LINE-1 retrotransposons translocates to the nucleus under stress, disrupts nuclear envelope integrity, and contributes to neurodegenerative features in neurons.

FLN-2 functions in parallel to LINC complexes and Cdc42/actin pathways during P-cell nuclear migration through constricted spaces in Caenorhabditis elegans

The paper identifies FLN-2 as a third pathway mediating P-cell nuclear migration in C. elegans, functioning alongside LINC complexes and CDC-42/actin pathways to maintain nuclear envelope integrity during migration through constricted spaces.

The plant nuclear lamina disassembles to regulate genome folding in stress conditions

The paper shows that the plant nuclear lamina disassembles under stress, altering chromatin organization and transcriptional profiles.