Barrier Structure and Function Studies

Low testosterone promotes anxiety through astrocytic mitochondrial remodelling at the nucleus accumbens blood-brain barrier

The paper shows low testosterone increases anxiety by altering astrocytic mitochondrial structure and function at the nucleus accumbens blood-brain barrier in rats.

Wnt/beta-catenin signaling regulates Vascular Endothelial Growth Factor (VEGF) receptors in central nervous system endothelial cells

This study shows that Wnt/beta-catenin signaling modulates VEGF receptor levels in CNS endothelial cells, thereby regulating VEGF signaling.

In vivo discovery of blood-brain barrier opening small molecules with FishNAP

This study identifies small molecules that temporarily open the blood-brain barrier in zebrafish and mice, with reversible effects and potential for drug delivery.

Transthyretin Contributes to Hippocampal Blood-Brain Barrier Recovery Following Intestinal Inflammation, with Reduced Expression in Alzheimer's Disease

The paper investigates how intestinal inflammation affects the blood-brain barrier (BBB) and identifies transthyretin (TTR) as a key factor in BBB recovery, with reduced TTR expression linked to Alzheimer's disease.

Predicting Cerebral Pericyte Contractility Across Experimental and Physiological Conditions: an in-silico framework

The paper introduces a computational framework to predict cerebral pericyte contractility under various experimental and physiological conditions, validated across multiple interventions.

Ang2 and TAT targeting of leptomeningeal disease by the intravenous and intrathecal routes: a comparative analysis

This study compares the targeting efficiency of Ang2 and TAT peptides via intravenous and intrathecal routes in leptomeningeal disease models, finding TAT achieves better targeting in specific regions.

Dynamic knowledge representation of blood brain barrier activation, injury and restitution with a novel agent-based model

The paper introduces a novel agent-based model of the blood brain barrier to simulate its activation, injury, and restoration dynamics.

Combinatorial Modulation of Wnt, STAT3, TGF-β, and Tie2 Pathways Drives Brain Endothelial Cell-Like Differentiation from hiPSCs

The paper identifies signaling pathways that drive the differentiation of brain endothelial cells from hiPSCs in vitro.

Design and Application of a μSiM Outer Blood-Retinal Barrier (OBRB) Model as a Drug Development Tool

The paper develops a μSiM-OBRB model to measure drug transport across the outer blood-retinal barrier, showing drug permeability and disease-relevant phenotypes.

Brain pericytes exhibit spatially organized and dynamically regulated molecular heterogeneity

This study identifies spatial and dynamic molecular heterogeneity in adult mouse brain pericytes across anatomical regions, vascular positions, aging, ischemia, and circadian phases.

Defining a leptomeningeal blood cerebrospinal fluid barrier as a specialized vascular interface

The paper identifies leptomeningeal endothelial cells as a distinct vascular population with unique molecular and functional properties compared to cortical blood brain barrier endothelial cells.

Delivery of small interfering RNA and antisense oligonucleotides across the blood-brain barrier with monovalent transferrin receptor 1 binding VHH-Fc fusion proteins

This paper develops a VHH-Fc fusion protein to deliver siRNAs and ASOs across the BBB in rodents and NHPs, showing that binding affinity, DAR, and conjugation site affect delivery efficiency.

Disruptions in glucose and amyloid-beta transport in mouse models manifesting metabolic syndrome

This study investigates how metabolic syndrome in mice affects amyloid-beta and glucose transport across the blood-brain barrier, linking insulin resistance to Alzheimer's disease pathology.

Gut-immune signaling drives blood-brain barrier damage in pediatric allogeneic stem cell transplant

This study identifies immune signaling and gut microbiota-derived metabolites as drivers of blood-brain barrier damage in pediatric allo-HSCT recipients.

A novel in vitro assay for quantifying endothelial cell and pericyte interactions

This paper introduces a high-throughput co-culture system to study endothelial cell and pericyte interactions and validates its use in assessing disease-relevant stimuli and therapeutics.

Impact of Neurons on Group B Streptococcus Interactions at the Blood Brain Barrier

This study shows that neuronal-endothelial communication enhances blood-brain barrier resistance to Group B Streptococcus (GBS) infection by reducing bacterial adherence and invasion.

Modulating the Novel miR-23b-Wnt/β-catenin Axis Strengthens Endothelial Barrier Properties

This study identifies miR-23b as a negative regulator of BBB integrity and shows that anti-miR-23b enhances barrier function through multiple signaling pathways and improves outcomes in a stroke model.

On-demand, reversible blood-brain barrier opening via electrical activation of piezoelectric nanoparticles for targeted brain drug delivery

Electrical activation of piezoelectric nanoparticles reversibly opens the blood-brain barrier for minimally invasive drug delivery to targeted cortical regions.

Progranulin haploinsufficiency remodels the cerebral microvasculature and neurovascular unit

This study shows that progranulin deficiency causes microvascular and neurovascular unit changes in FTD-GRN, affecting perfusion and signaling pathways.

Astrocytic and endothelial GLUT1 restoration improves brain glucose homeostasis in GLUT1 deficiency syndrome

This study shows that restoring GLUT1 in both astrocytes and endothelial cells improves glucose homeostasis and cognitive function in GLUT1 deficiency syndrome.

Molecular mechanism of action of a blood brain barrier shuttle antibody

This study determines the cryo-EM structure of a brain shuttle antibody bound to the transferrin receptor and shows how its binding features affect avidity, pH sensitivity, and brain delivery.

Shared and disease-specific human brain vascular signatures in Alzheimer's disease, frontotemporal dementia, and Huntington's disease

The paper identifies shared and disease-specific vascular signatures in Alzheimer's, frontotemporal dementia, and Huntington's disease using transcriptomic analysis of brain vessel datasets.

FRAGMENT MORPHOMETRY ANALYSIS AND SAME-COLOR-CHANNEL SEPARATION ENABLE OBJECTIVE QUANTIFICATION ACROSS BBB MODELS

The paper introduces two image-analysis pipelines to objectively quantify BBB integrity in vitro and in vivo, showing reduced ZO-1 fragment area and fragmentation ratio in glycocalyx knockdown models and altered markers in aged and Alzheimer's mice.

Bradycardia inhibits brain vessel mural cell differentiation via reducing mechanosensory and Jag2-Notch signaling

This study shows that bradycardia reduces mural cell numbers in developing brain vasculature by impairing mechanosensory and Jag2-Notch signaling, and that restoring Jag2b rescues this defect.

The Calcium Pump ATP2B1/PMCA1 Regulates CNS Vascular Development by Facilitating Norrin- and WNT7A/B-induced Frizzled4 signaling

This study identifies ATP2B1 as a regulator of Norrin- and WNT7A/B-induced Frizzled4 signaling in endothelial cells, showing its role in CNS vascular development.

Cryo-electron tomography reveals paracellular claudin-15 pores at the tight junction

The paper uses cryo-electron tomography to directly visualize paracellular claudin-15 pores in tight junctions, confirming their structure and spacing.

Birth by Cesarean Section Remodels Neonatal Brain Barriers and Associated Immune and Metabolic Pathways in Mice

This study shows that cesarean section in mice alters brain barrier function, immune profiles, and metabolism in the neonatal brain, highlighting birth mode's impact on early brain development.

HTRA1 deficiency in COL4A1 mutant hiPSC-derived astrocytes: a convergent mechanism of cerebral small vessel disease

This study identifies HTRA1 deficiency in COL4A1 mutant astrocytes as a mechanism underlying BBB dysfunction in cerebral small vessel disease.

Vascular and synaptic proteomes reveal blood-brain barrier disruption and postsynaptic remodeling in human temporal lobe epilepsy

This study identifies molecular changes in vascular and synaptic proteomes of human temporal lobe epilepsy, revealing BBB disruption and synaptic remodeling.

Syncytial coupling of mid-capillary pericytes underlies seizure-associated electro-metabolic signaling

The paper investigates electrical signaling between mid-capillary pericytes and endothelial cells in the neurovascular unit, showing syncytial coupling and its role in transmitting seizure-associated signals without relying on VGCCs.