Cellular transport and secretion

Clathrin recruitment and assembly of phosphorylated B cell receptor is impaired in human diffuse large B cell lymphoma

The paper investigates BCR signaling defects in ABC DLBCL, finding impaired clathrin recruitment of phosphorylated CD79A and spatial segregation from BCR components.

Blast fungus unconventional effector secretion sorting requires pre-Golgi compartment protein MoGrh1

The paper identifies MoGrh1 as a pre-Golgi protein required for unconventional secretion in the blast fungus Magnaporthe oryzae, linking autophagy and ERGIC to effector secretion during host infection.

SNX2, but not SNX1, regulates the hepatocyte growth factor receptor MET in HCT116 colorectal cancer cells

This study shows that SNX2, but not SNX1, regulates MET endosomal trafficking and signaling in HCT116 cells, leading to enhanced AKT activation and poorer CRC patient survival.

Contact of mobile cis-Golgi network with ER exit sites executes COPI-mediated receptor recycling

The paper shows COPI-mediated receptor recycling occurs during the transient contact between the mobile cis-Golgi network and ER exit sites in budding yeast.

A dsRNA Virus Converts Early Endosomes into Stable Replication Organelles Through Maturation Arrest and Retrograde Transport

This study reveals how birnaviruses use VP3 to remodel endosomes into replication compartments through PtdIns(3)P-dependent mechanisms, showing conservation and host adaptation across IBDV and IPNV.

A Kiss-and-Ride Mechanism for Lipid Exchange at Membrane Contact Sites

This study identifies a 'kiss-and-ride' mechanism for lipid transfer mediated by Osh6, showing that transient membrane tethering facilitates cargo extraction and transport.

Mechanical regulation of endocytosis by protein droplet capillary forces

This study investigates how protein droplet capillary forces regulate clathrin-mediated endocytosis by stalling and then driving membrane invagination.

Distinct roles for TANGO1S domains in maintaining ER-Golgi architecture

This study investigates the functional roles of TANGO1S domains in maintaining ER-Golgi architecture by analyzing domain-deletion mutants in knockout cells.

Quantification of lipid sorting during clathrin-mediated endocytosis

This study quantifies lipid sorting during clathrin-mediated endocytosis and finds that lipid asymmetry in the plasma membrane explains observed partitioning, with minimal selectivity for cytoplasmic leaflet lipids compared to non-vesicular trafficking.

LRRC57/RABIN is a presynaptic inhibitor of Rab GTPases in glutamatergic neurons

The paper identifies LRRC57/RABIN as a presynaptic inhibitor of Rab GTPases in glutamatergic neurons, showing it regulates neurotransmitter release and prevents epileptiform activity.

Clathrin adaptors drive phase separation in endocytosis and trafficking

This study shows that clathrin adaptors Ent5 and Sla2 undergo liquid-liquid phase separation, with distinct structural features driving condensate formation and function in endocytosis and trafficking.

Signal peptidase complexes set species-specific rules for signal peptide cleavage

The paper shows that human signal peptides are inefficiently cleaved by yeast SPCs due to differences in SPC-lipid interactions and membrane thinning profiles.

Traffickome infers directed, compartment-resolved membrane trafficking from proteomic data

Traffickome infers directed membrane trafficking between cellular compartments from proteomic data, achieving high accuracy in predicting trafficking transitions.

Phosphoregulation of hierarchical protein condensate formation during clathrin-mediated endocytosis

This study identifies two biophysical classes of proteins involved in clathrin-mediated endocytosis and shows how phosphorylation modulates their condensation dynamics.

GAPDH is tethered to axonal transport vesicles by S-acylation

The paper identifies S-acylation of GAPDH on cysteine 247 by ZDHHC5 and ZDHHC17 as essential for its association with axonal transport vesicles.

Selectivity and dynamics of VAP family tether exchange across membrane contact sites

The paper investigates VAP family tether selectivity and dynamics at membrane contact sites, revealing distinct MCS footprints and compensatory mechanisms in cellular homeostasis.

Lipids Regulate Export of Lysosomal Enzymes from the Endoplasmic Reticulum

The paper shows that de novo lipogenesis regulates lysosomal enzyme export from the ER by promoting Arf1 myristoylation and retrograde vesicle trafficking.

In vivo imaging uncovers an abundant but rarely active pool of plant ARP2/3 and its link to exocytosis and endocytosis

The paper uses live-cell imaging to show that plant ARP2/3 complexes exist in a large, dynamic, and mostly inactive pool, with functional links to exocytosis and endocytosis.

Two intramolecular interfaces control the WDR44 conformational switch that regulates Rab11 binding

The paper identifies two intramolecular interfaces that regulate WDR44 conformation and Rab11 binding, showing how pathogenic mutations disrupt this regulation.

Competing calcium sensors orchestrate various patterns of synaptic transmission

The paper develops a biophysical model to show how synaptotagmin-1 and synaptotagmin-7 interact to shape synaptic release patterns through calcium binding and exocytotic kinetics.

Phosphorylation of the C-terminus of PI4KA inhibits lipid kinase activity

This study identifies phosphorylation of Y2090 in PI4KA as an inhibitory mechanism that suppresses its lipid kinase activity through conformational changes and reduced membrane recruitment.

The VPS9-family GEF VINE activates Ypt10 in a late endosomal Rab cascade

This paper identifies VINE as a dual Rab regulator that activates Ypt10 and suppresses Vps21 to control late endosomal Rab signaling in yeast.

Adaptor interactions trigger Pan1 self-assembly during clathrin-mediated endocytosis in budding yeast

This study shows that Pan1 self-assembles into condensates during clathrin-mediated endocytosis in budding yeast, driven by adaptor interactions and regulated by specific protein domains.

Endosomal membrane state governs trafficking and cell migration under TFEB control

This study shows TFEB regulates endosomal membrane fluidity and cargo trafficking, affecting cell migration and adhesion in bladder cancer.

Clathrin differentially adapts its trimerisation domain during mammalian evolution to traffic the insulin-responsive GLUT4 glucose transporter

The paper identifies an alternatively-spliced form of CHC17 (CHC17-SAS) that compensates for the absence of CHC22 in some vertebrates, enabling GLUT4 trafficking.

PI(3)P Signaling by VPS34 Complex II Orchestrates Macropinocytosis

This study identifies VPS34 complex II as a regulator of macropinocytosis through PI(3)P signaling, showing its distinct role from class I PI3K activity.

The rhomboid-like pseudoprotease TMEM115 defines a two-factor mechanism for Rab6A effector recruitment

This study identifies TMEM115 as a two-factor component with Rab6A that recruits the golgin TMF1 to the Golgi apparatus, with implications for protein trafficking and disease.

Cdc42 coordinates apical membrane formation and secretory granule maturation through v-SNARE maintenance in salivary acinar cells

This study shows Cdc42 maintains v-SNAREs VAMP2 and VAMP4, which are critical for apical membrane formation and secretory granule maturation in salivary acinar cells.

The PP2A phosphatase associates with the Arabidopsis TRAPPII tethering complex and dephosphorylates a TRAPPII-derived phosphopeptide in vitro

This study identifies PP2A phosphatase as a potential dephosphorylating enzyme for TRAPPII subunit TRS120 in Arabidopsis, linking kinase-phosphatase balance to membrane trafficking.

Multiple overlapping SNARE complexes drive endosome maturation in Drosophila nephrocytes

The paper identifies multiple SNARE-dependent pathways driving endosome maturation in Drosophila nephrocytes, revealing a network of parallel fusion routes rather than a single linear pathway.