Telomeres, Telomerase, and Senescence

Fission yeast RPA-TERT-Tpz1TPP1 complex promotes telomere extension and suppresses telomere recombination

This study identifies a RPA-Trt1TERT-Tpz1TPP1 complex in fission yeast that promotes telomere extension and suppresses recombination through specific protein interactions.

Telomere variant sequences encode the genetic blueprint for allele-specific telomere length

The paper identifies heritable telomere variant sequences that determine allele-specific telomere length and shows their role in telomere maintenance and inheritance.

Machine learning-based definition of cellular senescence reveals pro-senescence potential implication in lung adenocarcinoma

This study introduces PreCSenM, a machine learning model that quantifies cellular senescence and identifies HDAC inhibitors as senescence inducers in lung adenocarcinoma.

FAβ-gal: an automated fluorescence-based quantification of the senescence-associated beta-galactosidase X-gal assay

This paper introduces FAβ-gal, a fluorescence-based method to quantify SA-β-gal activity using X-gal, improving accuracy and sensitivity over traditional colorimetric assays.

Fragmentation Patterns of Human Telomeric Chromatin in Plasma cfDNA

This study shows that cfDNA can map telomere chromatin structure, revealing age-related and disease-specific changes in telomeric footprints and gene signatures.

A Sequential Assembly Mechanism for Stable Cdc13 Dimerization on Telomeric DNA

This study shows Cdc13 initially binds telomeres as a monomer, then forms a stable dimer, revealing a sequential mechanism for telomere protection and length regulation.

A replicated patient-specific component of tumour telomere length across two pan-cancer cohorts

This paper shows that tumour telomere length is influenced by patient-specific telomere length, replicating this across two cancer cohorts.

The function of human PIF1 in G quadruplex formation and replication stress response at ALT telomeres

This study investigates the role of human PIF1 in ALT telomere maintenance, showing its involvement in DNA damage, G4 formation, and replication stress response, with implications for cancer therapy.

Mitochondrial dysfunction as a hallmark of brain senescence in telomerase-deficient mice

This study identifies mitochondrial dysfunction as a key feature of brain senescence in telomerase-deficient mice, linking telomere attrition to impaired mitochondrial function and region-specific neuronal vulnerability.

A Standardized In Vitro Platform for Senolytic Drug Discovery in Human Musculoskeletal Cells

This paper develops a standardized in vitro model for senolytic drug screening using human intervertebral disc cells and validates a metabolic activity assay as a scalable method to assess senolytic activity.

Telomeric amplicons of SUL1 and Y' in yeast are generated by microhomology-mediated break induced replication occurring in cis

The paper identifies a mechanism of gene amplification in yeast involving microhomology-mediated break-induced replication (mmBIR) at telomeres, leading to tandem duplication of chromosomal ends.

The HES1-SOD1 Antagonism Shapes Senescence Heterogeneity and Impacts Metastatic Relapse of Circulating Tumor Cells

This study identifies two HES1-expressing senescent CTC subpopulations with distinct metabolic and clinical outcomes, showing HES1-SOD1 antagonism influences metastatic relapse.

Targeting CyclinD1-CDK6 to Mitigate Senescence-Driven Inflammation and Age-Associated Functional Decline

This study shows that targeting CCND1-CDK6 reduces DNA damage, inflammation, and age-related decline in mice by suppressing SASP and ISG expression.

Nanopore sequencing to measure chromosome end-specific telomere lengths in human cells

This paper introduces a method using Oxford Nanopore long-read sequencing to measure chromosome-specific telomere lengths in human cells.

The BLM DHBN domain and structure-selective nucleases drive mitotic arrest-dependent telomere deprotection under WRN and TRF2 control

This study identifies the BLM DHBN domain and the roles of WRN, MUS81, and GEN1 in mitotic arrest-dependent telomere deprotection, showing TRF2's protective role.

PCC1 treatment reshapes ribosomal, lysosomal and membrane-lipid transcriptional programs in therapy-induced senescent human stromal cells

The paper analyzes the transcriptomic effects of PCC1 treatment on senescent human stromal cells, identifying gene expression changes in ribosomal, lysosomal, and membrane-lipid pathways.

Cellular senescence is associated with age-related loss of liver zonation and hepatocyte function

This study shows that cellular senescence disrupts liver zonation and hepatocyte function with age by altering gene expression and cell signaling.

Rapid evolution and functional divergence of the monkeyflower Mimulus lewisii telomerase

This study investigates rapid evolution of TERT in Mimulus lewisii and shows its functional divergence in binding TR paralogs, leading to telomere sequence changes and dominance in hybrids.

Suppression of early pro-inflammatory senescent signature post-radiotherapy mitigates chronic bone damage

This study shows that early suppression of the pro-inflammatory senescent secretome (SASP) with ruxolitinib reduces chronic bone damage after radiotherapy.

La1: an evolutionarily conserved player in the Arabidopsis telomerase complex

This paper identifies AtLa1 as an essential RNA-binding factor for Arabidopsis telomerase function, revealing its role in telomerase complex assembly and highlighting evolutionary conservation with yeast and ciliate proteins.

Characterizing the SASP-Dependent Paracrine Spreading of Senescence Between Human Brain Cell Types

The paper identifies cell-type-specific SASP ligand-receptor pairs that mediate paracrine senescence spreading in human brain cells and shows that targeting these factors can prevent senescence spread.

Disease mutations in the N-terminal extension and α 2'-helix of dyskerin do not abolish dimerization but disrupt binding to the telomerase RNA

The paper shows that disease mutations in dyskerin's N-terminal extension and α2-helix do not prevent dimerization but impair hTR binding, contributing to X-DC pathogenesis.

Cytoplasmic DNA Sensing Links LINE-1 Expression to Neuronal Senescence in Alzheimer's Disease

This study identifies LINE-1 retrotransposons as drivers of neuronal senescence in Alzheimer's disease through cytoplasmic DNA sensing and the cGAS-STING pathway.

Human length telomeres restrict the regenerative potential of hematopoietic stem cells in mice

The paper shows that human-length telomeres in mice restrict hematopoietic stem cell regenerative potential under stress conditions.

A CRISPR/Cas9-induced blunt-end telomere system in S. pombe reveals RNase H2-dependent RNA primer removal at the terminal Okazaki fragment of lagging telomeres

The paper introduces a CRISPR/Cas9 system in S. pombe to study telomere replication, revealing RNase H2-dependent RNA primer removal in lagging-strand telomeres.

Senescent cells cluster CTCF on nuclear speckles to sustain their splicing program

This paper identifies senescence-induced CTCF clusters (SICCs) and shows they sustain the senescence splicing program by repurposing nuclear speckle components.

Lysosomal Profiling with LysoTracker for Quantitative Assessment of Cellular Senescence in Human Fibroblasts

This paper introduces a live-cell imaging protocol using LysoTracker Deep Red to quantify lysosomal remodeling as a marker of cellular senescence in human fibroblasts.

Molecular and functional characterization of telomeric repeat-containing RNAs in Chinese hamster ovary cells

This study characterizes TERRA and ARIA telomeric RNAs in CHO cells, showing ARIA regulates telomeric DNA integrity by preventing ssDNA formation at ITSs.

Oncogene inactivation-induced senescence facilitates tumor relapse

This study shows that oncogene inactivation-induced senescence both limits tumor growth and promotes relapse by creating a pro-recurrence microenvironment.

Esc1-mediated anchoring regulates telomere clustering in response to metabolic changes.

The paper identifies Esc1-mediated telomere anchoring as a mechanism regulating telomere clustering in yeast during metabolic shifts to quiescence.