Metabolism, Diabetes, and Cancer

Ampk alpha2 T172 Activation Dictates Exercise Performance and Energy Transduction in Skeletal Muscle

This study shows that activating AMPK alpha2 T172 in skeletal muscle improves exercise performance and energy transduction, and its disruption mimics type 2 diabetes features.

Alpha Parvin Promotes Glucose Uptake and Metabolism in Skeletal Muscle with Minimal Influence on Hepatic Insulin Sensitivity

This study shows that Parvin in skeletal muscle is essential for insulin-stimulated glucose uptake and mitochondrial function, while having little effect on liver insulin sensitivity.

TAS1R3 regulates GTPase signaling in human skeletal muscle cells for glucose uptake.

This study shows TAS1R3 regulates glucose uptake in skeletal muscle via a Rac1 and phospho-cofilin pathway, independent of IRS1-AKT and Galphaq/11, and identifies it as a potential therapeutic target for T2D.

The fate of pyruvate dictates cell growth by modulating cellular redox potential

The paper shows that pyruvate metabolism affects cell growth in post-mitotic cells by altering redox balance and metabolic pathways, despite active growth signals.

Common and distinct roles of AMPKγ isoforms in small-molecule activator-stimulated glucose uptake in mouse skeletal muscle

This study identifies distinct roles of AMPKγ isoforms in glucose uptake in skeletal muscle, showing γ1 is required for ADaM site activator-stimulated uptake but not AMP-binding activators.

AMPK acts to remove immune barriers to CD8+ T cell-mediated immunity against hepatocellular carcinoma

This study shows that aldometanib activates AMPK to enhance CD8+ T cell infiltration and control hepatocellular carcinoma growth in immune-competent mice.

Mechanism and cellular actions of the potent AMPK inhibitor BAY-3827

This paper describes the mechanism and cellular effects of the potent AMPK inhibitor BAY-3827, showing it selectively blocks AMPK-dependent functions.

Fasting-induced hepatic gluconeogenesis is compromised in Anxa6-/- mice

This study shows that Anxa6-/- mice have impaired fasting-induced hepatic gluconeogenesis, leading to hypoglycaemia and reliance on lipid oxidation.

microRNA-1 Regulates Metabolic Flexibility in Skeletal Muscle via Pyruvate Metabolism

This study shows miR-1 regulates metabolic flexibility in skeletal muscle by controlling pyruvate metabolism, with loss of miR-1 causing metabolic inflexibility and reduced endurance.

Identification of Rapaglutin E as An Isoform-specific Inhibitor of Glucose Transporter 1

This paper identifies rapaglutin E as a specific inhibitor of GLUT1 and explores its binding mechanism compared to rapaglutin A.

Coordinated Regulation of Renal Glucose Reabsorption and Gluconeogenesis by mTORC2 and Potassium

This study shows that mTORC2 and dietary potassium coordinate renal glucose reabsorption and gluconeogenesis through independent mechanisms, with implications for diabetes management.

MLX phosphorylation stabilizes the ChREBP-MLX heterotetramer on tandem E-boxes to control carbohydrate and lipid metabolism

This study shows MLX phosphorylation is required for ChREBP-MLX heterotetramer formation on ChoREs, regulating carbohydrate and lipid metabolism.

An Enzyme-Linked Immunosorbent Assay (ELISA)-based Activity Assay for AMP-Activated Protein Kinase (AMPK)

The paper describes an ELISA-based assay for measuring AMPK activity, offering a simple, sensitive alternative to radioactive and commercial methods.

Exploring the effects of metformin on the body by urine proteome

This study identifies 166 differential proteins in rat urine after metformin treatment, revealing potential mechanisms and novel pathways associated with its effects.

Integration of metabolomic and transcriptomic analyses reveals novel regulatory functions of the ChREBP transcription factor in energy metabolism

This study integrates transcriptomic and metabolomic analyses to reveal novel regulatory functions of ChREBP in mitochondrial metabolism and cellular energy balance beyond its canonical roles in carbohydrate and lipid metabolism.

ULK1-regulated AMP sensing by AMPK and its application for the treatment of chronic kidney disease

This study identifies ULK1-dependent AMPK activation mechanisms and shows MK8722 improves renal function in CKD.

HNF4α-TET2-FBP1 axis contributes to gluconeogenesis and type 2 diabetes

This study identifies an HNF4-TET2-FBP1 axis that regulates gluconeogenesis and shows metformin's effect on this pathway in type 2 diabetes.

From hyperinsulinemia to cancer progression: how diminishing glucose storage capacity fuels insulin resistance

The paper links hyperinsulinemia to cancer progression by showing how reduced glucose storage capacity leads to insulin resistance and affects tumor response to therapy.

Chemical imaging unveils mitochondria as the major site of medicinal biguanide accumulation within cells

This study uses chemical imaging to show that metformin accumulates primarily in mitochondria within cells.

The genetic and dietary landscape of the muscle insulin signalling network

This study uses phosphoproteomics to show how genetic background and diet influence insulin signalling in muscle, revealing key regulators of insulin responsiveness and glycolysis in insulin resistance.

Leveraging genetic diversity to identify small molecules that reverse mouse skeletal muscle insulin resistance

The paper identifies small molecules that reverse insulin resistance in mouse skeletal muscle by integrating systems genetics and chemical screening.

A genetic strategy to measure insulin signaling regulation and physiology in Drosophila

The paper develops a genetic method to measure tissue-specific insulin signaling dynamics in Drosophila using phosphorylated Akt quantification.

Metformin use history and genome-wide DNA methylation profile: potential molecular mechanism for aging and longevity

This study examines the association between metformin use and DNA methylation profiles in blood samples to explore potential epigenetic mechanisms of aging and longevity.

In vivo transomic analyses of glucose-responsive metabolism in skeletal muscle reveal core differences between the healthy and obese states

This study uses multiomic analysis of skeletal muscle in obese and healthy mice to reveal differences in metabolic regulation by metabolites and genes after glucose administration.

Hepatocyte Rap1a Contributes To Obesity- and Statin-Associated Hyperglycemia

This study shows that Rap1a suppresses hepatic glucose production and that its inhibition, via statins, contributes to hyperglycemia in obesity.

Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter

The paper develops a sensitive AMPK activity reporter, ExRai AMPKAR, to study subcellular AMPK dynamics and reveals differential regulation of AMPK activity at lysosomes, mitochondria, and the nucleus.

The Mixed Blessing of AMPK Signaling in Cancer Treatments

This study develops a computational model to explore the conflicting roles of AMPK in cancer by simulating metabolic signaling pathways and their effects on tumorigenesis.

Solute carrier 5A5 regulates systemic glucose homeostasis by mediating glucose absorption in the Drosophila midgut

This study identifies dSLC5A5 as the first Drosophila glucose transporter in the midgut and shows it regulates glucose absorption and systemic homeostasis through endocytosis and lysosomal degradation.

BRAF controls the effects of metformin on neuroblast cell divisions in C. elegans

This study shows metformin suppresses neuronal Q-cell divisions in C. elegans independently of AMPK but through BRAF-MAPK pathway inhibition.

ADP is the dominant controller of AMP-activated protein kinase activity dynamics in skeletal muscle during exercise

The paper develops a mathematical model of AMPK signaling dynamics in skeletal muscle during exercise and finds that ADP, not AMP, is the dominant controller of AMPK activity due to its higher concentration.