Cholesterol and Lipid Metabolism

TOMM40 Knockdown in Macrophages Inhibits Oxidized LDL-induced NLRP3 Activation and Promotes LXR-β Mediated Cholesterol Transporter Gene Expression

This study shows that TOMM40 knockdown in macrophages inhibits NLRP3 activation and increases LXR-β mediated cholesterol transporter gene expression, potentially reducing atherogenesis.

Cholesterol-Responsive Interaction of NFE2L1-INSIG1 Controls VLDL Secretion and MASH Pathogenesis

This study identifies a cholesterol-responsive interaction between NFE2L1 and INSIG1 that sustains cholesterol balance and prevents MASH by promoting VLDL secretion.

Ammonia-mediated Conformational Dynamics in SCAP for SREBP Activation

This study uses molecular dynamics simulations to show ammonia induces conformational changes in SCAP that activate the SREBP pathway by promoting dissociation from Insig.

REGULATION OF HDL DYSFUNCTIONALITY BY PHOSPHATIDYLETHANOLAMINE LINKS POLY-UNSATURATED FATTY ACIDS WITH ATHEROSCLEROTIC CARDIOVASCULAR DISEASES

This study shows that arachidonic acid-containing phosphatidylethanolamine (ARA-PE) impairs HDL function, promoting atherosclerosis, while eicosapentaenoic acid-PE (EPA-PE) restores HDL function and may reduce ASCVD risk.

Restoring Cholesterol Efflux in Vascular Smooth Muscle Cells Transitioning into Foam Cells Through Liver X Receptor Activation

This study shows that activating LXR enhances cholesterol efflux from vascular smooth muscle cells (VSMCs) transitioning into foam cells, improving reverse cholesterol transport to feces and suggesting LXR agonists and ACAT inhibitors as potential therapies for atherosclerosis.

A high-content microscopy drug screening platform for regulators of the extracellular digestion of lipoprotein aggregates by macrophages

This paper develops a high-content microscopy drug screening platform to identify compounds that inhibit lysosome exocytosis during digestive exophagy in macrophages.

Endogenous and fluorescent sterols reveal the molecular basis for ligand selectivity of human sterol transporters

The paper profiles fluorescent steroid probes across ten STPs to uncover selectivity differences and establishes a screening panel to identify new Aster-B inhibitors.

The contribution of mitochondria-associated ER membranes to cholesterol homeostasis

This study shows that SR-B1-mediated cholesterol uptake from HDL stimulates MAM formation, which suppresses de novo cholesterol biosynthesis.

Lipid droplet surface promotes three-dimensional morphological evolution of non-rhomboidal cholesterol crystals

This study shows how cholesterol crystals form non-rhomboidal shapes on lipid droplets and develop into three-dimensional sheet-like structures through interfacial transport and lipid droplet components.

TOMM40 regulates hepatocellular and plasma lipid metabolism via an LXR-dependent pathway

This study shows that TOMM40 regulates lipid metabolism in hepatocytes through an LXR-dependent pathway, affecting plasma lipid levels and hepatic lipid droplet accumulation.

Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferase

The paper identifies gliflozins, sucrose, and flavonoids as activators of LCAT using in silico predictions and in vitro assays, highlighting their potential in treating LCAT deficiency and atherosclerosis.

A distributed integral control mechanism for the regulation of cholesterol concentration in the human retina.

The paper provides a systems-level description of retinal cholesterol homeostasis through a chemical reaction network and identifies integral control mechanisms regulating cholesterol in the human retina.

Targeting Liver Epsins Ameliorates Dyslipidemia in Atherosclerosis through Inhibition of Proprotein Convertase Subtilisin/Kexin Type 9-Mediated Low-density Lipoprotein Receptor Degradation

This study shows that targeting liver epsins reduces atherosclerosis by preventing PCSK9-mediated LDLR degradation and improving LDL-C clearance.

ABCA1 and apoA-I dependent 12-hydroxyeicosatetraenoic acid efflux regulates macrophage inflammatory signaling

The paper investigates how ABCA1 and apoA-I facilitate 12-HETE efflux from macrophages, reducing inflammation by modulating intracellular HETE levels.

Impaired cholesterol transport from aged astrocytes to neurons can be rescued by cannabinoids.

This study shows that aging impairs cholesterol transport from astrocytes to neurons, which can be rescued by cannabinoids.

Systematic Evaluation of Lecithin:Cholesterol Acyltransferase Binding Sites in Apolipoproteins via Peptide Based Nanodiscs: Regulatory Role of Charged Residues at Positions 4 and 7

This study identifies molecular determinants of LCAT binding to apolipoproteins using simulations, revealing key residues that influence LCAT activity on different lipoproteins.

Sterol O-acyltransferase (SOAT/ACAT) activity is required to form cholesterol crystals in hepatocyte lipid droplets

This study shows that SOAT1-mediated cholesterol esterification is required for cholesterol crystal formation in hepatocyte lipid droplets, linking it to MASH pathogenesis.

Metabolome-wide association of carotid intima media thickness identifies FDX1 as a determinant of cholesterol metabolism and cardiovascular risk in Asian populations.

Bilirubin is a new ligand for nuclear receptor Liver Receptor Homolog-1.

This study identifies bilirubin as a direct ligand for LRH-1, showing it modulates LRH-1 activity and gene regulation in liver cells.

The LEPIS-HuR-TMOD4 axis regulates hepatic cholesterol homeostasis and accelerates atherosclerosis

This study identifies the LEPIS-HuR-TMOD4 axis as a regulator of hepatic cholesterol homeostasis and atherosclerosis in ApoE-/- mice.

Functional omics of ORP7 in primary endothelial cells.

This study investigates ORP7's functions in HUVECs using omics approaches, finding changes in lipid metabolism, inflammation, and angiogenesis upon ORP7 inhibition.

Maternal sterol 27-hydroxylase is crucial for securing fetal development

This study shows that maternal Cyp27a1 is essential for normal pregnancy and fetal development by preventing toxic 7-HC accumulation.

HDL regulates TGFβ-receptor lipid raft partitioning, restoring contractile features of cholesterol-loaded vascular smooth muscle cells

This paper shows that cholesterol-loaded vascular smooth muscle cells (VSMCs) lose TGFβ signaling and contractile features, but HDL-mediated cholesterol efflux restores signaling and contractile markers.

Hepatic retromer is essential for systemic cholesterol homeostasis by regulating lysosomal cholesterol metabolism

This study shows that hepatic VPS35 deficiency disrupts lysosomal cholesterol metabolism, leading to increased cholesterol levels in plasma and liver.

Mapping the dynamic cell surface interactome of high-density lipoprotein reveals Aminopeptidase N as modulator of its endothelial uptake

The paper identifies a cell surface protein complex, the HDL synapse, that modulates HDL uptake into endothelial cells, with AMPN playing a key role.

Regulation of cellular LDL uptake by PROX1 and CHD7

This study identifies PROX1 and CHD7 as regulators of LDL uptake, with PROX1 directly enhancing LDLR expression and CHD7 acting through an LDLR-independent mechanism.

Decoding functional high-density lipoprotein particle surfaceome interactions

The paper identifies HDL receptors and their interactions in human endothelial cells, revealing MERTK as a novel co-receptor for HDL binding and uptake.

AUF-1 knock down in mice overarches butyrate driven hypo-cholesteraemia by conjuring AUF-1-Dicer-1-miR122 hierarchy

The paper investigates how butyrate affects cholesterol levels through the AUF-1-Dicer-1-miR122 pathway in mice, showing that AUF-1 knockdown increases miR122 and reduces serum cholesterol.

Genome-scale CRISPR screening for modifiers of cellular LDL uptake

The paper uses genome-scale CRISPR screening to identify genetic modifiers of LDL uptake in hepatocytes, finding several new genes involved in this process.

The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form

This paper identifies a proteasomally truncated form of the cholesterol synthesis enzyme SM that is constitutively active and resistant to cholesterol-induced degradation.