Congenital heart defects research

flt1 inactivation promotes zebrafish cardiac regeneration by enhancing endothelial activity and limiting the fibrotic response

This study shows that inactivating flt1 in zebrafish enhances cardiac regeneration by improving vascularization and reducing fibrosis.

Interpreting variants associated with congenital heart disease with a regulatory map of the human fetal heart

This study creates a gene regulation map of the human fetal heart to interpret genetic variants associated with congenital heart disease and cardiac traits, identifying valvular interstitial cells as key regulators.

Spns1-dependent endocardial lysosomal function drives valve morphogenesis through Notch1-signaling.

This study identifies a role for Spns1-dependent lysosomal function in endocardial cells during cardiac valve development, showing its impact on Notch1 signaling and valve morphology.

Spatio-temporal dynamics of the fibrotic niche in cardiac repair

This study uses single-cell RNA-sequencing and spatial transcriptomics to analyze the spatio-temporal dynamics of the fibrotic niche in cardiac repair after ventricular injury in adult mice, identifying key cell interactions and regulatory mechanisms.

A Machine Learning Classifier to Identify and Prioritise Genes Associated with Cardiac Development

The paper develops a machine learning classifier to identify mouse genes involved in cardiac development and prioritizes them for potential human congenital heart disease (CHD) causation.

Harnessing the regenerative potential of interleukin11 to enhance heart repair

This study identifies IL11's dual role in heart regeneration and fibrosis, showing that combining IL11a induction with ERK inhibition can enhance regeneration while reducing fibrosis.

A Foxf1-Wnt-Nr2f1 cascade promotes atrial cardiomyocyte differentiation in zebrafish

This study identifies a conserved regulatory network involving Foxf1, Wnt signaling, and Nr2f1a that promotes atrial cardiomyocyte differentiation in zebrafish.

Zebrafish arterial valve development occurs through direct differentiation of second heart field progenitors

This study shows that zebrafish arterial valve development is conserved with mammalian valve leaflets and can be used for testing BAV variants.

The mesodermal source of fibronectin is required for heart morphogenesis and cardiac outflow tract elongation by regulating cell shape, polarity, and mechanotransduction in the second heart field

This study identifies fibronectin as a key regulator of cardiac outflow tract elongation by modulating cell behavior in the second heart field.

Hypoxia regulate developmental coronary angiogenesis potentially through VEGFR2- and SOX17-mediated signaling

The paper investigates how hypoxia affects coronary vessel development in mouse hearts and identifies VEGF-R2- and SOX17-mediated signaling as potential downstream pathways.

Transient formation of collaterals contributes to the restoration of the arterial tree during cardiac regeneration in neonatal mice

This study shows that transient collateral arteries form during cardiac regeneration in neonatal mice, aiding in LAD repair and perfusion restoration, while persistent collaterals are seen in non-regenerating hearts.

Hypomorphic NOTCH1 Expression Alters Cardiomyocyte Cellular Architecture in Hypoplastic Left Heart Syndrome

This paper uses CRISPR-edited human iPSCs with hypomorphic NOTCH1 to model hypoplastic left heart syndrome, identifying auranofin as a potential therapeutic.

Rare homozygous cilia gene variants identified in consanguineous congenital heart disease patients

The paper identifies rare homozygous variants in cilia genes associated with congenital heart defects in consanguineous Pakistani patients.

Identification of Long Non-coding RNA Candidate Disease Genes Associated with Clinically Reported CNVs in Congenital Heart Disease

This study identifies lncRNA candidate genes associated with CNVs in CHD patients, revealing their significant role in heart development and disease.

Myocardial Endoglin Regulates Cardiomyocyte Proliferation and Cardiac Regeneration

The paper identifies Endoglin as an inhibitor of cardiomyocyte proliferation and shows its role in cardiac regeneration through genetic and mechanistic studies.

Cardiac Fibroblasts regulate myocardium and coronary vasculature development via the collagen signaling pathway

This study identifies the role of cardiac fibroblasts in regulating myocardium and coronary vasculature development through the collagen signaling pathway using genetic ablation and single-cell RNA sequencing.

MicroRNA miR-92b-3p regulation of a cardiovascular gene regulatory network in the developing branchial arches

The paper identifies miR-92b-3p as a regulator of cardiovascular gene regulatory networks in mouse branchial arches, showing its role in downregulating cardiac transcription factors during development.

FZD2 inhibits YAP and prevents cell cycle reentry in adult murine cardiomyocytes

This study shows FZD2 inhibits YAP and prevents adult murine cardiomyocytes from reentering the cell cycle, and its deletion improves heart function and reduces scarring after myocardial infarction.

Postnatal xanthine metabolism regulates cardiac regeneration in mammals

This study shows that postnatal xanthine metabolism regulates cardiac regeneration by linking redox state to cardiomyocyte proliferation and regenerative potential in mice.

Llgl1 mediates timely epicardial emergence and establishment of an apical laminin sheath around the trabeculating cardiac ventricle.

This study identifies Llgl1's role in regulating the timely establishment of the epicardium and apical laminin sheath during cardiac ventricle development in zebrafish.

Cardiac Development Long non-coding RNA (CARDEL) is activated during human heart development and contributes to cardiac specification and homeostasis

The paper identifies CARDEL as a long non-coding RNA involved in human cardiac specification and homeostasis through in vitro differentiation and gene expression analysis.

BMP signaling promotes heart regeneration via alleviation of replication stress

This study shows BMP signaling alleviates replication stress in cardiomyocytes, enabling heart regeneration in zebrafish and potentially applicable to humans.

Inherent Metabolic Adaptations in Adult Spiny Mouse (Acomys) Cardiomyocytes Facilitate Enhanced Cardiac Recovery Following Myocardial Infarction

This study identifies unique metabolic adaptations in Acomys cardiomyocytes that enhance cardiac recovery after myocardial infarction compared to Mus musculus.

scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular building blocks of the primary pacemaker

The paper uses scRNA-seq to analyze zebrafish heart development, identifying 18 cell populations and two genes critical for pacemaker function and heart rate regulation.

The HH-GLI2-CKS1B network regulates the proliferation-to-maturation transition of human cardiomyocytes

This study identifies the HH-GLI2-CKS1B network as a regulator of the proliferation-to-maturation transition in human cardiomyocytes, with implications for cardiac regeneration.

Epigenetic delineation of the earliest cardiac lineage segregation by single-cell multi-omics

The paper uses single-cell multi-omics to study early cardiac lineage specification in mouse embryos, identifying key transcription factors and regulatory networks.

Endocardial HDAC3 is required for myocardial trabeculation

This study shows that endocardial HDAC3 is essential for myocardial trabeculation by regulating TGFβ3 expression through miR-129-5p repression in mice.

Endocardial primary cilia and blood flow are required for regulation of EndoMT during endocardial cushion development

The paper shows that endocardial primary cilia and blood flow regulate EndoMT during cushion development by modulating KLF4 expression.

Contraction-induced endocardial id2b plays a dual role in regulating myocardial contractility and valve formation

This study identifies id2b as a gene regulated by biomechanical forces in zebrafish hearts, showing its role in both myocardial contractility and valve formation through interactions with tcf3b and nrg1.

Developmental Dynamics of Human Cardiogenesis: A multi-omic reference and its disruption in Trisomy 21

This study develops a multi-omic reference of human cardiac development and identifies disruptions in Trisomy 21 hearts.