Congenital heart defects research

BMP7 promotes cardiomyocyte regeneration

This study identifies BMP7 as a key growth factor promoting cardiomyocyte proliferation in both mouse and zebrafish models, with potential therapeutic applications for heart regeneration.

Identification of novel buffering mechanisms in aortic arch artery development and congenital heart disease.

The paper identifies a buffering mechanism in aortic arch artery development that compensates for reduced endothelial cell numbers, preventing congenital heart disease.

Toll-like receptor 3 orchestrates a conserved mechanism of heart regeneration

This study identifies TLR3 as a conserved mediator of cardiac regeneration in zebrafish, showing its role in immune cell recruitment and DNA repair after myocardial injury.

A Synergistic Desmin-SPARC Axis Regulates Cardiac Stem Cell Differentiation and Promotes Cardiomyogenesis through Autocrine Regulation

The paper shows that desmin induces SPARC secretion in cardiac stem cells, promoting cardiomyogenesis through autocrine signaling and enhancing myocardial transcription factor expression.

Distinct features of the regenerating heart uncovered through comparative single-cell profiling

This study compares cardiac injury responses in zebrafish and medaka to identify features distinguishing regenerative and non-regenerative heart responses.

Notch3 is a genetic modifier of NODAL signalling for patterning asymmetry during mouse heart looping

The paper identifies Notch3 as a genetic modifier of Nodal signaling that influences heart looping asymmetry in mice.

Cardiac Outflow tract septation defects in a DiGeorge syndrome model respond to Minoxidil treatment.

The paper shows that Minoxidil treatment reduces cardiac outflow tract septation defects in a Tbx1 mutant mouse model of DiGeorge syndrome by inhibiting collagen cross-linking.

morphoHeart: a novel quantitative tool to perform integrated 3D morphometric analyses of heart and ECM morphology during embryonic development

morphoHeart provides 3D morphometric analysis of heart and ECM in live embryos, showing regional ECM expansion linked to atrial lumen development.

The MEK-ERK signaling pathway promotes maintenance of cardiac chamber identity

This study identifies the MEK-ERK signaling pathway's role in maintaining ventricular and atrial cardiac chamber identities through downstream FGF signaling and upstream regulation of nkx2.5.

The changing morphology of the ventricular walls of mouse and human with increasing gestation.

The paper quantitatively and morphologically analyzes the development of ventricular walls in mouse and human hearts, finding that slow trabecular layer growth, not compaction, drives morphological changes.

Single cell transcriptional landscape of long non-coding RNAs orchestrating mouse heart development

The paper identifies lncRNAs involved in mouse heart development using single-cell transcriptomics and co-expression analysis, revealing cell-specific functional insights and associations with disease-related genes.

Single Cell Multimodal Analyses Reveal Epigenomic and Transcriptomic Basis for Birth Defects in Maternal Diabetes

The paper uses single-cell multimodal analyses to show how maternal diabetes alters epigenomic and transcriptomic states in cardiac and craniofacial progenitors, leading to developmental defects.

Single nucleus transcriptome of a "Super RV" shows increased insulin and angiogenesis signaling

The paper presents the single-nucleus transcriptome of a systemic right ventricle from a patient with congenitally corrected transposition of great arteries, identifying increased insulin and angiogenesis signaling as potential beneficial adaptations to pressure overload.

Multi-chamber cardioids unravel human heart development and cardiac defects

This paper develops a multi-chamber cardioid platform to study human heart development and defects by generating compartment-specific gene expression, morphology, and function.

Spatial transcriptomics reveals novel genes during the remodelling of the embryonic human arterial valves

This study uses spatial transcriptomics to analyze human embryonic arterial valves, identifying novel genes and pathways involved in valve development and highlighting limitations in current genomic databases.

The myocardium utilizes Pdgfra-PI3K signaling to steer towards the midline during heart tube formation

This study identifies PI3K signaling as an intrinsic mechanism guiding myocardial cell movement during heart tube formation in zebrafish.

Chromatin state transition underlies the temporal changes in gene expression during cardiomyocyte maturation

This study identifies RNF20-mediated chromatin state transitions as critical for time-ordered gene expression during cardiomyocyte maturation and links its disruption to congenital heart disease.

Hand2 represses non-cardiac cell fates through chromatin remodeling at cis-regulatory elements

This study identifies Hand2's role in repressing non-cardiac cell fates through chromatin remodeling at cis-regulatory elements in zebrafish cardiac progenitors.

The Chordate Origins of Heart Regeneration

This paper shows that the tunicate Ciona can regenerate its heart after complete ablation, revealing evolutionary insights into regenerative cardiogenesis in chordates.

mTORC1 Regulates the Metabolic Switch of Postnatal Cardiomyocytes During Regeneration

This study shows that mTORC1 inhibition accelerates the metabolic switch from glycolysis to fatty acid oxidation in neonatal heart injury, impairing regeneration.

Using Drosophila to model a variant of unknown significance in the human cardiogenic gene Nkx2.5

The paper uses Drosophila to model a human Nkx2.5 variant, showing it causes DNA binding defects and reduced cofactor interaction, leading to cardiac differentiation issues.

Sin3a Associated Protein 130kDa, sap130, plays an evolutionary conserved role in zebrafish heart development

The paper investigates the role of sap130a in zebrafish heart development, showing it regulates SHF cell accretion and maturation, leading to ventricular hypoplasia.

β1 integrins regulate cellular behaviors and cardiomyocyte organization during ventricular wall formation

This study investigates the role of β1 integrins in regulating cardiomyocyte behavior and organization during ventricular wall formation in mice, finding that their deletion disrupts tissue architecture and trabeculae formation.

Defining Cardiac Nerve Architecture During Development, Disease, and Regeneration

The paper defines cardiac nerve architecture during development, disease, and regeneration using 3D modeling and imaging, showing regenerating myocardium reestablishes physiological innervation.

A transient modified mRNA encoding Myc and Cyclin T1 induces cardiac regeneration and improves cardiac function after myocardial injury

This study shows that modified mRNA encoding Myc and Cyclin T1 can induce cardiomyocyte proliferation and improve cardiac function after myocardial injury in mice.

Conserved Chamber-Specific Polyploidy Maintains Heart Function in Drosophila

This study identifies conserved polyploidy asymmetry between cardiac chambers in Drosophila and humans, showing its role in maintaining heart function and its implications for heart failure.

Heterogeneity and Functional Analysis of Cardiac Fibroblasts in Heart Development

This study identifies developmental heterogeneity in cardiac fibroblasts and their functional roles through single-cell RNA sequencing and ablation experiments.

Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration

The paper identifies distinct cardiac resident macrophage subpopulations critical for zebrafish heart regeneration through comparative single-cell profiling.

Mitochondrial MICOS complex genes, implicated in hypoplastic left heart syndrome, maintain cardiac contractility and actomyosin integrity

The paper identifies mitochondrial MICOS complex genes, CHCHD3 and CHCHD6, as contributors to hypoplastic left heart syndrome through functional studies in Drosophila and genetic analysis of patient cohorts.

Cardiac competence of the paraxial head mesoderm fades concomitant with a shift towards the head skeletal muscle programme

The paper shows that the paraxial head mesoderm in chicken embryos retains cardiac competence until early head fold stages, after which Bmp signals drive a shift to head skeletal muscle programmes.