flow cytometry

Brain-wide reductions in cell size and volume emerge early during adulthood in Drosophila melanogaster.

This study identifies early brain-wide reductions in cell size and volume during adulthood in Drosophila melanogaster, linked to aging and modifiable by Myc overexpression.

Enrichment of Extracellular Vesicles Subsets from Diverse Biological Sources Using Conventional and Image-Based Fluorescence Activated Sorting

This paper develops and validates an optimized workflow for isolating and characterizing extracellular vesicle subsets from diverse sources using FACS and imaging techniques.

Targeting PIEZO1-TMEM16F Coupling to Mitigate Sickle Cell Disease Complications

This paper identifies PIEZO1-TMEM16F coupling as a mechanism driving phosphatidylserine exposure in sickle red blood cells and proposes targeting this pathway to reduce disease complications.

Targeting Tumor-intrinsic TAK1 triggers anti-tumor immunity and sensitizes pancreatic cancer to checkpoint blockade

This study identifies TAK1 as a therapeutic target in pancreatic cancer that enhances anti-tumor immunity and sensitizes tumors to checkpoint blockade.

A searchable database of publications using fluorescent probes and flow cytometry to study antigen-specific B cells

The paper creates a searchable database of publications using fluorescent probes and flow cytometry to study antigen-specific B cells, showing increased research focus on pathogens like SARS-CoV-2 and HIV.

A TLR7/9-IFNα-LDHB axis drives vital NET release and compromises antibacterial defense.

The paper identifies a TLR7/9-IFNα-LDHB axis that impairs NETosis in SLE, leading to bacterial susceptibility, and shows reversal with HCQ and IFNAR blockade.

Disease severity in coinfected hosts: the importance of infection order

This study shows that the order of infection with two parasites affects disease severity, with Py causing more severe symptoms after Hp infection due to impaired immune responses and increased anemia.

A deep single cell mass cytometry approach to capture canonical and noncanonical cell cycle states

This paper develops a deep single-cell mass cytometry approach to capture both canonical and noncanonical cell cycle states across diverse cell systems.

Tertiary lymphoid structures support the development of allergen-specific progenitor CD4+ T cells

This study identifies two distinct CD4+ T cell populations in inflamed lungs, showing that TCF1+ progenitor cells in tertiary lymphoid structures drive Th2 responses in allergic disease.

Characterization of the Cellular Immune Response to Group B Streptococcal Vaginal Colonization

This study investigates the cellular immune response to GBS vaginal colonization in mice, finding that neutrophils and γδ T cells contribute to host defense, while overall immune cell numbers remain stable.

Angiotensin II Infusion Promotes Activation and Selective Cytokine Responses in Activated CD4 and CD8 T cells

This study shows that angiotensin II infusion increases T cell activation markers and specific cytokine responses in CD4 and CD8 T cells in mice.

Chronic inflammation drives epididymal tertiary lymphoid structure formation and autoimmune fertility disorders

This study shows that chronic inflammation in the epididymis leads to tertiary lymphoid structure formation and autoimmune fertility issues in mice, with similar autoantibodies found in human infertile patients.

Stromal cells modulate innate immune cell phenotype and function in colorectal cancer via the Sialic acid/Siglec axis

This study shows stromal cell sialylation via the Sialic acid/Siglec axis suppresses innate immune cell function in CRC, and targeting this pathway restores immune activity.

A Bi-Specific T Cell-Engaging Antibody Shows Potent Activity, Specificity, and Tumor Microenvironment Remodeling in Experimental Syngeneic and Genetically Engineered Models of GBM

This study evaluates a bispecific T cell-engaging antibody in immunocompetent GBM models, showing improved survival, TME remodeling, and immune memory induction.

Single-cell-level digital twins for preterm birth prevention strategies

The paper introduces SIMCO, a single-cell digital twin framework for evaluating PTL prevention drugs, identifying tetrahydrofolate, maprotiline, and aspirin-lansoprazole as top candidates.

Ridge-assisted Micro Positioning of Cells and Particles in a Microchannel

This paper introduces RAMP, a microfluidic method for precisely positioning cell streams in microchannels across a wide flow rate range, independent of particle size.

Self-consistent automatic retrieval of single cell rotation enables highly reliable holo-tomographic flow cytometry

This paper introduces a self-consistent method for automatically retrieving single cell rotations in holo-tomographic flow cytometry, achieving full automation and superior accuracy.

Targeting neoantigens conserved across organs and species overcomes tumor immune escape

This study identifies conserved neoantigens across species and tissues that elicit strong immune responses and suppress tumor growth through vaccination.

Infant CD4 T-cell response to SARS-CoV-2 mRNA vaccination is restricted in cytokine production and modified by vaccine manufacturer

This study compares CD4 T-cell cytokine responses to SARS-CoV-2 mRNA vaccines in infants and adults, finding restricted cytokine production in infants and differences by vaccine type.

Focal adhesion pathway inhibition is the central axis of macrophage phenotypic responses to monoclonal antibody therapy in aggressive lymphoma via high-throughput screening and high-content imaging

This study identifies focal adhesion pathway inhibition as a key mechanism enhancing macrophage phagocytosis and improving anti-CD20 monoclonal antibody therapy in aggressive lymphoma through high-throughput screening and high-content imaging.

Regulation of TCR-induced Transcriptional Kinetics by Interleukin 2-inducible T Cell Kinase (ITK) in CD8+ T cells

This study shows ITK differentially regulates early and late TCR transcriptional responses by affecting the kinetics and termination of activation signals in CD8+ T cells.

Potentiating CAR-T bystander killing by enhanced Fas/FasL signaling mitigates antigen escape in heterogeneous tumors

This study shows that enhancing Fas/FasL signaling improves CAR-T cell bystander killing against antigen-negative tumor cells, reducing antigen escape and improving therapeutic outcomes.

The anti-virus T cell response dominates the anti-cancer response in oncolytic virus therapy

This study shows oncolytic virus therapy primarily elicits anti-virus T cell responses rather than anti-cancer responses, explaining limited clinical efficacy in metastatic disease.

High-Fidelity Simulation of Cell Adhesion and Flow with Coarse Grain Modelling: A Numerical and Experimental Approach

The paper develops a CFD-DEM framework to simulate RBC transport and adhesion in microchannels, validated against experiments for cell-substrate interaction studies.

Mechanisms of superior respiratory IgA responses against SARS-CoV-2 after mucosal vaccination

This study investigates respiratory IgA responses after SARS-CoV-2 infection, vaccination, or hybrid immunity, showing that mucosal booster vaccination enhances IgA and protective immunity.

An atlas of natural killer cell receptor expression on healthy donor T and NK lymphocytes

The paper maps NK cell receptor expression on T and NK lymphocytes in healthy donors, revealing patterns linked to donor sex, maturation, and activation.

In vitro and in vivo derived macrophages occupy distinct phenotypic states

The paper compares in vitro and in vivo macrophage phenotypes using mass cytometry, finding distinct surface marker expression patterns between macrophages derived from culture and those from tumor-associated environments.

Dynamics of Macrophage Polarization Reveal Unique Phenotypes During Mouse Postpartum Uterine Remodeling

This study examines macrophage polarization dynamics in mouse uterine tissues during postpartum remodeling, revealing shifts from M2 to M1 and back to M2, with unique mixed phenotype subsets.

Single-cell quantification of the microbiota by flow cytometry: MicFLY

The paper develops MicFLY, a flow cytometry method to quantify and characterize intestinal microbiota at the single-cell level, revealing E. coli unbound by IgG and IgA is associated with necrotizing enterocolitis.

Phagosome Maturation in Macrophages is Enhanced by p38α MAPK Signaling

The paper shows that specific ligands on phagocytosed cargo activate p38 MAPK signaling, enhancing phagosome maturation and lysosomal delivery in macrophages.