Tuberculosis Research and Epidemiology

Evaluating selection at intermediate scales within genes provides robust identification of genes under positive selection in M. tuberculosis clinical isolates

The paper evaluates positive selection in M. tuberculosis genes at three scales, finding that intermediate-scale analysis identifies the most credible candidate genes under selection.

Hyperthermia reduces antimicrobial tolerance of Mycobacterium marinum in biofilms and ex vivo zebrafish granulomas

This study shows hyperthermia enhances antimicrobial effectiveness against Mycobacterium marinum biofilms and granulomas by reducing drug tolerance.

Genome graphs reveal the importance of structural variation in Mycobacterium tuberculosis evolution and drug resistance

This study uses long-read sequencing and genome graphs to analyze structural variation in Mycobacterium tuberculosis, identifying SVs linked to drug resistance and virulence.

Mycobacterium tuberculosis growth arrest on propionate at acidic pH is suppressed by mutations in phoPR and pyrazinamide treatment

This study identifies mutations in phoPR and pyrazinamide treatment as factors suppressing Mtb growth arrest on propionate at acidic pH.

Repurposing ethacridine as a potent MMPL3 Inhibitor for the treatment of tuberculosis

This paper identifies ethacridine as a potent inhibitor of MmpL3, effective against drug-sensitive and resistant tuberculosis strains.

MTBseq-nf: Enabling Scalable Tuberculosis Genomics "Big Data" Analysis through a User-Friendly Nextflow Wrapper for MTBseq pipeline

The paper introduces MTBseq-nf, a Nextflow wrapper for the MTBseq pipeline, improving scalability and efficiency for tuberculosis genomics analysis.

Remission spectroscopy resolves the mode of action of bedaquiline within living mycobacteria

This study uses remission spectroscopy to show bedaquiline inhibits ATP synthase in mycobacteria, redirecting electron flux through cytochrome bd oxidase and increasing O2 consumption, without uncoupling.

Targeting Metabolic Vulnerabilities: Valinomycin Augments the Potency of Bioenergetic Inhibitors in Combatting Drug-Resistant and Dormant Mycobacterium tuberculosis

This study shows that valinomycin enhances the effectiveness of bioenergetic inhibitors against drug-resistant and dormant Mycobacterium tuberculosis by disrupting the proton motive force.

Inflammasome activation differences underpin different Mycobacterium tuberculosis infection outcomes

This study identifies differences in inflammasome activation pathways that correlate with varying outcomes of Mycobacterium tuberculosis infection.

Minimal immune cell subset differences in a cohort of close contacts of tuberculosis index cases

This study examines immune cell differences in close contacts of TB patients based on QFT status, finding minimal differences among subgroups but clear immune changes in active TB patients.

Diverse infection models demonstrate robust resistance of Mycobacterium tuberculosis to innate immunity

This study shows that Mycobacterium tuberculosis is resistant to innate immune clearance even under conditions that enhance innate immunity.

Rv3400 is a phosphoglucomutase required for trehalose metabolism in Mycobacterium tuberculosis

This study shows that Rv3400, a β-phosphoglucomutase in M. tuberculosis, is essential for trehalose metabolism, as its deletion increases β-D-glucose 1-phosphate and causes growth defects on trehalose.

Mycobacterium tuberculosis overcomes phosphate starvation by extensively remodelling its lipidome with phosphorus-free lipids

This study shows Mycobacterium tuberculosis remodels its lipidome to survive phosphate starvation by using phosphorus-free lipids and host-derived phosphate sources.

Control of antigen presentation on MHC-I by a bacterial secretion system

The paper shows that Mycobacterium tuberculosis uses its ESX-1 secretion system to present antigens on MHC-I, bypassing host pathways like autophagy.

The phenotypic landscape of the mycobacterial cell

The paper performs a genome-wide CRISPRi screen in Mycobacterium smegmatis to uncover novel gene functions and drug targets related to the cell envelope.

Clinical and bacterial determinants of unfavorable tuberculosis treatment outcomes: an observational study in Georgia

This study identifies patient and bacterial factors associated with unfavorable tuberculosis treatment outcomes in Georgia using genomic and clinical data from 4,536 patients.

Mycobacterial surface shedding drives bystander cells response during early intracellular infection

This study shows mycobacteria shed surface components during early infection, which affect both infected and bystander host cells.

Identification of determinants of high-fidelity DNA synthesis in M. smegmatis DnaE1 through in silico and in vivo approaches

The paper identifies amino acid changes in M. smegmatis DnaE1 that affect DNA polymerase fidelity through in silico and experimental approaches.

Random barcode transposon-site sequencing in Mycobacterium tuberculosis reveals the functions of uncharacterized genes

This study uses random barcode transposon-site sequencing in Mycobacterium tuberculosis to identify gene functions, revealing novel phenotypes and metabolic pathways.

Enrichment of a CD4-CD8- NK-like cytotoxic Vδ1/3 T cell subset in tuberculosis disease

This study identifies an enriched NK-like cytotoxic Vδ3 T cell subset in TB patients using single-cell RNA sequencing, showing persistent transcriptomic differences post-treatment.

Cross-species blood transcriptional correlates of BCG-mediated protection against tuberculosis include innate and adaptive immune processes

This study identifies cross-species blood transcriptional correlates of BCG-mediated protection against tuberculosis using a systems modeling framework, linking innate and adaptive immune processes to vaccine efficacy in humans and non-human primates.

Mapping unsolved lipidomes accelerates lipid discovery in major bacterial pathogens

The paper maps unknown lipids in bacterial pathogens using lipidomic methods, revealing new biosynthetic products linked to virulence.

An explainable artificial intelligence framework reveals mutations associated with drug resistance in Mycobacterium tuberculosis

The paper introduces xAI-MTBDR, an AI framework that identifies drug resistance mutations in MTB and predicts resistance, outperforming existing methods and revealing new resistance markers.

NINJ1 mediates necrosis of Mycobacterium tuberculosis infected human macrophages

This study identifies NINJ1 as a mediator of plasma membrane rupture in Mtb-infected macrophages, showing it operates independently of canonical cell death pathways and requires the ESX-1 secretion system.

Defining the Mycobacterium tuberculosis Pangenome and Suggestions for a New Composite Reference Sequence

This study generates 50 complete Mtb genomes, identifies core and accessory gene clusters, and proposes a new pangenome reference resource to improve TB research.

Protection against reinfection with Mycobacterium tuberculosis extends across heterologous Mtb lineages

The study shows that prior Mtb infection protects against reinfection with a genetically distinct strain in macaques, suggesting broad vaccine efficacy across Mtb lineages.

Split-luciferin Assay for Real-time Measurement of Cytosolic Molecular Accumulation in Live Mycobacteria

This paper introduces D-CLAP, a split-luciferin assay to measure real-time cytosolic accumulation of compounds in live mycobacteria, showing that modifying carboxylates increases accumulation and antimycobacterial activity.

Tax1bp1 enhances bacterial virulence and promotes inflammatory responses during Mycobacterium tuberculosis infection of alveolar macrophages

This study shows Tax1bp1 promotes Mtb growth in alveolar macrophages and enhances inflammatory responses, while restricting Mtb in bone marrow-derived macrophages.

The ID93 + GLA-3M-052-LS vaccine candidate elicits mucosal and systemic immunogenicity and protective efficacy against Mycobacterium tuberculosis challenge in BCG-primed Collaborative Cross inbred mice

This study evaluates a TB vaccine candidate, ID93 + GLA-3M-052-LS, showing enhanced mucosal and systemic immunity and protection against Mtb in BCG-primed mice.

A Specialized CD107a+ Macrophage Subset Drives Selective Mycobacterial Phagocytosis

This paper identifies a CD107a+ macrophage subset with enhanced phagocytic ability for mycobacteria and shows its role in tuberculosis defense.