Advanced Electron Microscopy Techniques and Applications

High-resolution cryoEM of nucleosomes in nuclear extracts of mammalian cells

The paper presents a method for high-resolution cryoEM of nucleosomes in mammalian cell nuclear extracts without requiring thin sectioning.

Adaptive Milling: imaging feedback-driven automated fabrication of consistently thin cryo-lamellae

This paper introduces Adaptive Polishing, a machine learning-driven method for improving the consistency and thinness of cryo-lamellae during focused ion beam milling.

Programmable gold nanoparticle conjugates enable precise AMPA receptor localization at brain synapses by cryo-ET

FPGA-based scanner and SerialEM server for 4D-STEM Electron Tomography

The paper develops an FPGA-based scanner and SerialEM server for 4D-STEM electron tomography, enabling automated complex protocols and demonstrating system performance with a mitochondrial tomogram.

Shaping cryogenic 3D volume imaging by pFIB ion species and milling voltage

This study investigates how ion species and milling voltage affect cryo-pFIB-SEM image quality, showing that optimal settings depend on specimen composition.

EM3DFold: accurate de novo protein and nucleic acid model building for cryo-EM maps using language model-powered deep learning

EM3DFold improves cryo-EM model building for proteins, nucleic acids, and complexes using a language model-powered deep learning framework, achieving high accuracy on benchmark datasets.

Depth-resolved analysis reveals target-dependent cryo-FIB damage profiles

The paper analyzes depth-dependent structural damage caused by cryo-FIB milling using apoferritin as a reporter, showing damage varies with ion type and molecular target.

A 3D Soft X-Ray Structural Atlas of Multicellular Cable Bacteria

This study creates a 3D structural atlas of cable bacteria using SXT and other techniques, revealing intracellular organization and conductive network features.

Cryo-EM Sample Preparation with Soft-Landing and Laser Flash Melting

This paper demonstrates a method to rehydrate proteins after soft-landing for cryo-EM by flash melting, enabling native configurations and improving sample preparation.

SGFP-Grid Split GFP Graphene Grids

The paper introduces a split-GFP graphene grid platform for selective protein capture in cryo-EM, demonstrating improved enrichment and a 2.58 Å cryoEM reconstruction of GFP11-tagged apoferritin.

Tailocins are recurrent contaminants in ex vivo amyloid extraction

The paper identifies tailocins as contaminants in ex vivo amyloid preparations and proposes strategies to detect and avoid them in cryo-EM.

Specimen-Dependent Sampling and Signal Limitations Govern the Effectiveness of Low-Magnification Super-Resolution in Cryo-EM

This study compares low-magnification super-resolution and physical-pixel workflows in cryo-EM, showing that effectiveness depends on specimen-specific signal and sampling limitations.

Cryo-ET and Sub-Volume Analysis of Fibrillar Pathology in the Neurodegenerative Human Brain

This paper uses cryo-ET to study amyloid fibrils in neurodegenerative brain tissue, revealing ultrastructural details of fibril polymorphs and their association with disease.

Plant-adapted OTO staining improves ultrastructural imaging by transmission electron microscopy

The paper introduces a modified OTO staining method for TEM that improves imaging of plant tissues by reducing artifacts and enhancing structural preservation.

CryoConvNeXt enables robust identification of low-abundance molecular species in experimental cryo-EM data

CryoConvNeXt improves identification of low-abundance molecular species in cryo-EM data by using deep learning with self-training, outperforming conventional methods in minority-species recall.

Cryo-EM structure of detergent-bound Vibrio cholerae MakA captures a candidate intermediate between the soluble form and mature assembly of an α-pore-forming toxin

The paper reports a cryo-EM structure of detergent-bound Vibrio cholerae MakA, revealing its conformational changes and role in pore assembly.

MemBrain v2: an end-to-end tool for the analysis of membranes in cryo-electron tomography

MemBrain v2 couples a generalist U-Net cryo-ET membrane segmenter, a mesh-based picker reaching 91% F1 for photosystem II from one annotated membrane, and geodesic particle statistics.

CryoFlex characterizes structural motion between conformations directly from cryo-EM density maps

CryoFlex quantifies structural motion between cryo-EM conformational states directly from density maps, achieving high accuracy and outperforming registration baselines.

Multimodal Protein Retrieval via Joint Representation Learning from Sequences and Cryo-EM Density Maps

This paper develops a multimodal framework to align protein sequences with cryo-EM density maps using joint representation learning, achieving strong retrieval performance across species and resolutions.

DAQplugin: Interactive Deep Learning-Based Validation of Cryo-EM Protein Models in ChimeraX

DAQplugin provides real-time deep learning-based validation of cryo-EM protein models in ChimeraX, identifying residue errors and offering correction guidance.

Volume Electron Microscopy of Cortical Organoids: Methods for Region Identification, Connectome Reconstruction, and Organelle Segmentation

This paper establishes a workflow for applying volume electron microscopy to human cortical organoids, enabling micro-connectomic reconstruction at nanometer resolution.

Cryoprotectants-assisted plunge freezing of thick brain tissue specimens for targeted physiologically relevant cryo-imaging in situ

This paper develops a cryoprotectant-assisted plunge freezing method for preserving thick mouse brain tissue for cryo-imaging, enabling high-resolution visualization of cellular structures and validating their physiological relevance.

Meso2EM: a cross-scale CLEM workflow linking mesoscale functional imaging to targeted electron microscopy

Meso2EM links mesoscale functional imaging to targeted electron microscopy by preserving neuron identity across scale changes using shared landmarks.

In-cell discovery and characterization of a non-canonical bacterial protein translocation-folding complex

The paper identifies and characterizes a non-canonical bacterial protein translocation-folding complex using cryo-electron tomography, proteomics, and structure prediction in Mycoplasma pneumoniae.

Pre-FIB Layer-Mapping Cryo Tomography (PLCT) for Depth-Resolved in Situ Structural Analysis of Multilayered Tissues

The paper introduces PLCT, a method for depth-resolved structural analysis of multilayered tissues using cryo-ET, and demonstrates its ability to resolve subcellular structures in retinal tissue.

Imaging large fields-of-view at high resolution in cryo-ET with square beam montaging

This paper introduces a pipeline for high-resolution cryo-ET imaging using square electron beams, demonstrating improved resolution and field-of-view by reducing radiation damage and overlap.

Overcoming air-water interface induced artifacts in Cryo-EM with protein nanocrates

This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.

Mind the Bend: Curved and Corrugated Cryo-Lamella for Improved Mechanical Resilience

This paper introduces curved and corrugated cryo-lamella designs to improve mechanical resilience and reduce lamella loss during electron microscopy sample preparation.

Atomic modeling of radiation damage in cryoelectron microscopy datasets

This paper develops a Bayesian framework to model radiation damage in cryo-EM datasets by inferring atomic displacements and structural changes during electron irradiation.

ARCHER: Amortized cross-specimen pose estimation for cryo-electron microscopy

This paper introduces ARCHER, a method for cross-specimen cryo-EM pose estimation that generalizes across structures using a contrastive classifier trained on protein structures.