Ubiquitin and proteasome pathways

Steric Gating Dictates Selective Activation of BIRC6 by UBA6 Over UBA1

This study identifies steric compatibility between BIRC6 and UBA6 as the mechanism enabling specific activation of BIRC6 by UBA6 over UBA1.

DUCs are C2 domain containing plant-specific deubiquitinases stabilizing endocytic cargo at the plasma membrane

The paper identifies plant-specific DUC deubiquitinases that stabilize endocytic cargo at the plasma membrane by cleaving K63-linked ubiquitin chains.

Ubiquitylome Rewiring by Bacterial E3 Ligases Reveals Multifaceted Host Subversion

This study identifies host proteins ubiquitylated by Salmonella NEL effectors, revealing their broad impact on host pathways and providing insights into bacterial subversion of host cell processes.

Design of linked-domain protein inhibitors of UBE2D as tools to study cellular ubiquitination

This paper designs a protein inhibitor for UBE2D enzymes to study their role in ubiquitination, showing it reduces Ub degradation and proteotoxic stress in HeLa cells.

Unveiling the crucial neuronal role of the proteasomal ATPase subunit gene PSMC5 in neurodevelopmental proteasomopathies

The paper identifies PSMC5 variants causing neurodevelopmental disorders and shows their impact on neuronal function and immune signaling, suggesting therapeutic targets.

The Mechanism of Histone Ubiquitylation by the ASB9-CUL5 Ubiquitin Ligase

This paper shows that the ASB9-CRL ubiquitin ligase polyubiquitylates histones H3 and H4, but not H2A and H2B, and that this process requires ARIH2 and occurs only on free histones.

Mechanisms of Ub-independent ODC recognition and translocation by the 26S proteasome revealing proteasome's functional adaptability

This study reveals the mechanism by which the 26S proteasome recognizes and degrades ODC without ubiquitin, through cryo-EM structures showing dynamic interactions and translocation processes.

Characterization of Endogenous and Epitope-Tagged Ubiquitin Expression and Ubiquitination Patterns at Drosophila Neuromuscular Junctions

The paper validates epitope-tagged UAS-Ubiquitin transgenes in Drosophila to accurately recapitulate endogenous ubiquitination patterns at neuromuscular junctions.

Structural landscape of AAA+ ATPase motor states in the substrate-degrading human 26S proteasome reveals conformation-specific binding of TXNL1

The paper solves high-resolution structures of TXNL1 bound to the human 26S proteasome, revealing conformation-specific binding and new insights into ATP hydrolysis and substrate translocation mechanisms.

Identification and characterization of a ubiquitin E3 RING ligase of the Chlamydia-like bacterium Simkania negevensis

This study characterizes a ubiquitin E3 RING ligase from Simkania negevensis, showing it generates K63- and K11-linked ubiquitin chains and interacts with specific E2 ligases.

Ubiquitin-dependent signal amplification in lipid saturation sensing

The paper investigates how Mga2 converts membrane physical cues into biochemical outputs via ubiquitylation, revealing a signal amplification mechanism involving E3 ligase autoubiquitylation.

Mechanistic basis for protein conjugation in a diverged bacterial ubiquitination pathway

This study characterizes the Bub bacterial ubiquitination-like pathway, revealing its unique biochemical mechanisms and structural similarities to eukaryotic ubiquitination.

The E3 ubiquitin ligase MGRN1 targets melanocortin receptors MC1R and MC4R via interactions with transmembrane adapters

This study identifies ATRN and ATRNL1 as transmembrane adapters that recruit MGRN1 to ubiquitinate and degrade MC1R and MC4R, expanding the known substrate repertoire of MGRN1.

Dynamic profiling of the aggresome processing pathway

This study identifies novel components of the aggresome and characterizes their interactions during aggresome processing pathway dynamics in HeLa cells.

The landscape of cellular clearance systems across human tissues and cell types is shaped by tissue-specific proteome needs

The paper characterizes the expression and essentiality of protein clearance systems across human tissues and cell types, revealing tissue-specific regulation and a core set of stably expressed genes.

Targeted disruption of linkage-specific ubiquitylation reveals a key role of K29-linked ubiquitylation in epigenome integrity

This study uses a ubiquitin replacement strategy to show K29-linked ubiquitylation regulates SUV39H1 stability and H3K9me3 levels in human cells.

Structural proteomics of the human ubiquitinome

This study uses structural proteomics to show that ubiquitination patterns differ between misfolded nascent proteins and mature proteins, influencing their degradation rates.

Structural basis of K11/K48-branched ubiquitin chain recognition by the human 26S proteasome

The paper reports cryo-EM structures of the human 26S proteasome bound to K11/K48-branched ubiquitin chains, revealing a multivalent recognition mechanism involving new binding sites for K11 and alternating K11-K48 linkages.

CK2α phosphorylates CHIP for oncogenesis: A novel post-translational switch in cancer

This paper identifies CK2α as a kinase that phosphorylates CHIP, promoting its degradation and oncogenesis, and shows that CK2 inhibition restores CHIP function and suppresses tumor growth.

Response of UBR-box E3 ubiquitin ligases and protein quality control pathways to perturbations in protein synthesis and skeletal muscle size

This study investigates how UBR-box E3 ubiquitin ligases respond to changes in protein synthesis and skeletal muscle size, finding increased abundance and activity of these ligases under conditions of muscle growth and regeneration.

Small-molecule allosteric activator of ubiquitin-specific protease 7 (USP7)

This study identifies and characterizes MS-8, a small-molecule activator of USP7, showing it activates mutant USP7 in a cellular context and impacts downstream proteins.

UBE2J2 sensitizes the ERAD ubiquitination cascade to changes in membrane lipid saturation

The paper shows UBE2J2 senses membrane lipid saturation by modulating its activity and interaction with E3 ligases, affecting ERAD ubiquitination.

Mapping the functional importance of site-specific ubiquitination across the human proteome

The paper maps the functional importance of site-specific ubiquitination across the human proteome by analyzing conservation, perturbation, and machine learning scores.

Role of AAA-ATPase Cdc48p in Peroxisomal Quality Control

The paper identifies Cdc48p as a key component of the RADAR pathway in yeast, which degrades misfolded peroxisomal receptors.

Assembly and substrate engagement mechanism of the bacterial proteasome activator Boa

This study defines the temperature-sensitive oligomerization mechanism of Bpa and elucidates its interaction with a surrogate substrate using biophysical techniques.

USP9X is a mechanosensitive deubiquitinase that controls tumor cell invasiveness and drug response through YAP stabilization

This study identifies USP9X as a mechanosensitive deubiquitinase that stabilizes YAP to enhance tumor cell invasiveness and drug resistance.

CRISPR screen identifies BAP1 as a deubiquitinase regulating SPIN4 stability

The paper identifies BAP1 as a deubiquitinase that stabilizes SPIN4 by counteracting degradation mediated by DCAF16.

Coronin1C SUMOylation modulates filopodia formation, neuritogenesis, and neuronal differentiation

This study shows that Coronin1C SUMOylation is essential for neurite formation and neuronal differentiation, and its deficiency leads to cytoplasmic aggregates associated with neurodegenerative diseases.

ISGylation Mechanism Uncovers Conformational Specificity for HECT-family E3 ligase

The paper reveals the conformational specificity of UbcH8[~]ISG15 for HECT-family E3 ligases, explaining its exclusive ISGylation activity and distinct mechanism from ubiquitination.

Ubiquitination of secretory granules promotes crinophagic degradation in Drosophila

The paper identifies ubiquitination as a key trigger for crinophagic degradation of secretory granules in Drosophila, mediated by Cnot4 and Usp7.