NF-κB Signaling Pathways

Integrator governs NF-κB dwell time on chromatin to drive inflammatory transcription

This study identifies the Integrator complex as a key factor stabilizing NF-κB chromatin residence, essential for inflammatory gene activation in mouse and human cells.

RNF213 M1-ubiquitinates damaged mitochondria to activate pro-inflammatory NF-κB signaling

This study identifies RNF213 as an E3 ligase that catalyzes M1-linked ubiquitination of damaged mitochondria, activating pro-inflammatory NF-κB signaling through NEMO recruitment.

3D Spatial Interactomics Maps the Dynamics of NF-κB Multiprotein Signalosomes in Single Cells

The paper maps NF-κB multiprotein signalosome dynamics in 3D using iseqPLA and scGPT, revealing spatial organization and disease-related changes in inflammation.

IKKα and IKKβ serve as kinase-independent scaffolds that prevent proteasomal degradation of NEMO

This study shows that IKKα and IKKβ stabilize NEMO via kinase-independent scaffolding, preventing its proteasomal degradation and NF-κB signaling.

Combination of enhanced gene activation and cell death causes lethal inflammatory pulmonary fibrosis

The paper shows that enhanced gene activation in specific LUBAC mutants leads to cell death and lethal lung fibrosis, revealing gene activation as a root cause of inflammatory disease.

IKK2/β mediated phosphorylation of transcription factor Ets2 at site(s) distal to DNA binding domain negatively modulates its DNA binding activity

The paper identifies S295 as a phosphorylation site on Ets2 regulated by IKK2/β, showing that phosphorylation inhibits DNA binding activity.

Cellular inhibitor of apoptosis protein (cIAP) is a convergent hub that couples BCR-CD40 signal dynamics to B cell fate

The paper identifies cIAP as a hub that couples BCR and CD40 signaling to B cell fate by sustaining canonical NF-κB signaling and restraining non-canonical pathways.

Glucose Metabolism Mediates Feedback Control of Innate Immune Signaling in Human Macrophages

This study identifies glucose metabolism's role in linking inflammatory and antiviral responses in human macrophages through the STAT1/NF-κB/IRF5 axis and PFKFB3.

OTUB1 controls marginal zone B-cell development by stabilizing RelA in a CD40-dependent manner

This study shows OTUB1 stabilizes RelA by preventing its ubiquitination and degradation, which is crucial for controlling marginal zone B-cell development through CD40-dependent NF-κB signaling.

Human rhinovirus 16 impairs macrophage cytokine secretion by disrupting NF-κB nuclear translocation and intracellular cytokine trafficking

This study shows HRV16 impairs macrophage cytokine secretion by disrupting NF-κB nuclear translocation and intracellular cytokine trafficking, with associated epigenetic changes.

Perturb-seq resolves physiologic programs and bidirectional regulation of non-canonical NF-κB signaling in epidermal organoids

The paper uses Perturb-seq in epidermal organoids to study non-canonical NF-κB signaling's role in keratinocyte differentiation.

Inactivation of HIF-P4H-1 Stabilizes IKKα, Modulates Non-Canonical NF-κB Signaling, and Sensitizes Cancer Cells to Cell Death

This study identifies HIF-P4H-1 as a regulator of non-canonical NF-κB signaling by stabilizing IKKα and shows its inhibition sensitizes cancer cells to death.

Limitations of EBV transformed human Raji B cells as a model for measuring canonical NF-κB activation

This study evaluates the limitations of using EBV-transformed Raji B cells to model inducible NF-κB activation, finding significant discrepancies in signaling responses compared to primary B cells.

Cryo-EM reveals alternative modes of dimerization driving activation of IKK

This paper uses cryo-EM to show that IKK2/β adopts distinct dimeric conformations that regulate its activation through structural plasticity and interactions with NEMO and polyubiquitin.

mTORC1 Directs TNF-Induced Life-or-Death Decisions via Complex I Destabilization

This paper shows mTORC1 regulates TNF-induced cell death by destabilizing complex I and promoting complex II formation, with implications for disease therapies.

Dredd-mediated cleavage of Kenny uncouples the IKK complex from selective autophagy to enable innate immunity

The paper shows that Dredd cleaves Kenny, releasing the IKK complex from autophagosomal repression to activate NF-κB and enable antibacterial immunity.

A rare human TNFAIP3 variant reveals how A20 abundance is regulated by TAX1BP1

The paper identifies a rare TNFAIP3 variant that shows A20 phosphorylation regulates its abundance via autophagy, not MALT1 degradation, through an interaction with TAX1BP1.

The binding of OTULIN restrains LUBAC activity to prevent TNF-driven immunopathology

This study shows that OTULIN's interaction with LUBAC limits its activity, preventing TNF-driven inflammation and immunopathology.

Pro-IL-1β activates NF-κB independently of maturation

This study shows pro-IL-1β activates NF-κB independently of maturation, using MyD88 and endocytosis, and identifies TLR7/8 as binding partners.

Signalome-wide mapping of the NFκB pathway in T-cells reveals novel targets for immunotherapy

This study maps the NFκB pathway in T-cells using CRISPR screens and identifies novel regulators that enhance cytotoxicity and cytokine production.

RGS10 differentially modulates NFκB subunit transcription and inflammatory cytokine profiles in peritoneal macrophages.

This study investigates how RGS10 modulates NFκB subunit transcription and inflammatory cytokine profiles in peritoneal macrophages through experimental stimulation and transcript analysis.

K27-linked ubiquitination restrains the NOD2-RIP2 signaling pathway

This study identifies K27-linked ubiquitination of RIP2 by XIAP as an inhibitory signal that modulates NOD2-RIP2 signaling and cytokine production.

The reovirus σ3 protein induces proteasomal degradation of IKKβ to modulate the host response to infection

This paper identifies reovirus σ3 as a viral protein that induces proteasomal degradation of IKKβ, reducing NF-κB activation and IFN-β expression to enhance viral replication.

A Neyman-Pearson Framework for Modeling Cellular Decision Making Using Single-Cell TNF-NF-κB Signaling Data

The paper applies a Neyman-Pearson framework to single-cell TNF-NF-κB signaling data to quantify decision-making performance and detect pathway failures.

TRAF6 coiled-coil domain mediates its trimerization

This study identifies the trimeric structure of TRAF6's coiled-coil domain using biophysical methods and computational tools.

Truncating RELA variants drive autoinflammation and autoimmunity by impairing the negative feedback control of NF-kB

This study identifies truncating RELA variants causing autoinflammation and autoimmunity by disrupting NF-κB feedback control and immune homeostasis.

Hypoxia-induced mitoROS triggers M1 linear ubiquitin chains and activates NF-κB signaling

The paper shows hypoxia-induced mitochondrial ROS leads to M1 ubiquitin chains, activating NF-κB signaling and increasing target gene expression in response to mitochondrial stress.

JNK integrates immune and stress signals to balance apoptosis and proliferation in airway progenitors

This study identifies JNK as a key integrator of immune and stress signals in airway progenitors, balancing apoptosis and proliferation to maintain tissue homeostasis during inflammation.

NF-κB driven inflammation mediates loss of upper airway epithelial tolerance to Streptococcus pneumoniae during influenza co-infection

This study shows that influenza co-infection disrupts epithelial tolerance to Streptococcus pneumoniae via NF-κB driven inflammation, leading to bacterial invasion and highlighting TRAF6-NF-κB as a therapeutic target.

MK2/p38/p53 suppress basal IL-1β and non-canonical NF-κB signaling

The paper shows that MK2/p38/p53 suppress basal IL-1β and non-canonical NF-κB signaling by regulating p53 and RelB cleavage.