RNA regulation and disease

Targeting an RNA Editor to Impede H3K27M+ Pediatric Gliomas

This study identifies ADAR as a key mediator of immune evasion in H3K27M+ pediatric gliomas and shows that ATRA can induce ADAR degradation, enhancing antiviral responses and tumor immunogenicity.

ADAR1 regulates alternative splicing through an RNA editing-independent mechanism.

This study shows ADAR1 regulates alternative splicing through both RNA editing-dependent and editing-independent mechanisms, with the latter having greater impact.

ADAR-Sense: an open-access, species-agnostic web tool for automated, user-customisable ADAR-based RNA sensor design

ADAR-Sense is a web tool for designing ADAR-based RNA sensors with customizable parameters, enabling efficient and flexible sensor creation for various applications.

Identification and Analysis of Novel RNA Editing Sites in Neurodegenerative Diseases Using Machine Learning Approaches.

This paper identifies novel A-to-I RNA editing sites in Alzheimer's disease using machine learning and finds they are enriched in exons and associated with neurodegeneration pathways.

APOBEC3A is the predominant global editor of cytosines in human mRNAs and in single-strand RNA viruses

The paper identifies APOBEC3A as the main RNA editor in human cells and viruses, showing its preference for specific motifs in mRNA and viral RNA.

Antisense lncRNA transcription promotes A-to-I RNA editing via intermolecular dsRNA in breast cancer

The paper identifies an antisense lncRNA pathway that enhances A-to-I RNA editing via intermolecular dsRNA, contributing to subtype-specific editing landscapes in breast cancer.

HAMMER: Hairpin-based APOBEC3A-mediated mRNA editing reporter

HAMMER is a luminescence-based assay that measures APOBEC3A-mediated RNA editing by detecting changes in luciferase activity ratios.

ApoFLARE: a luminescent reporter for direct quantification of APOBEC3A editing activity

The paper introduces ApoFLARE, a luminescent reporter that quantifies APOBEC3A editing activity in living cells, enabling time-resolved analysis of its kinetics and heterogeneity.

Primate lineage specification requires suppression of Alu hyperediting

The paper identifies ILF2/3 as a regulator of primate cell fate by suppressing Alu element editing, showing its role in preventing aberrant splicing and maintaining epigenetic stability.

Target-Stabilized Base Editors Enable Robust High-Fidelity RNA Editing

This paper introduces RECODE, a system that improves RNA editing specificity by using gRNA-regulated ADAR1 deaminase stability, achieving high editing efficiency for a FUS mutation.

Multiscale RNA editing analysis reveals cell type specific regulatory programs across disease states in acute myeloid leukemia

The paper identifies cell type-specific RNA editing patterns in AML across disease states using single-cell and pseudo-bulk analyses, revealing condition-specific regulatory programs.

Differential adenosine to inosine RNA editing of SINE B2 non-coding RNAs in mouse unveils a novel type of epi-transcriptome response to amyloid beta neuro-toxicity

The paper identifies increased A-to-I RNA editing of SINE B2 non-coding RNAs in mouse models of amyloid beta toxicity, linking it to stress-responsive gene expression.

The RNA Editome of Esophageal Squamous Cell Carcinoma Identifies an ADAR1-CDK13 Editing Feedback Loop Mediating cGAS-STING Activation in Early Tumorigenesis

This study identifies an ADAR1-CDK13 editing feedback loop in ESCC that enhances cGAS-STING signaling and worsens patient survival.

Engineered Allosteric RNA Editors Enable Compact, Stimulus-Responsive Post-Transcriptional Circuits

This paper introduces iDARs, inducible deaminases that enable stimulus-responsive RNA editing, allowing controlled protein translation or transcript degradation in response to various triggers.

Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity

This study shows that inositol hexakisphosphate (IP6) is an essential cofactor for ADAR1 activity, with its depletion reducing RNA editing and supplementation restoring it.

Pan-cancer RNA editing activity reveals complex editing functions and cancer immunotherapy biomarkers

The paper develops a computational framework to analyze RNA editing in cancer, identifying functions and biomarkers for immunotherapy.

Mitochondrial dsRNA: A Hidden Source of Immunogenic RNA in ADAR1 Deficiency

This study identifies mitochondrial dsRNA as a major endogenous dsRNA source in ADAR1 deficiency and proposes a mechanism linking ADAR1 dysfunction to immune activation.

Regulatable In Vivo Gene Expression via Adaptamers

This paper introduces an RNA-based system called adaptamer that allows precise, reversible control of gene expression using small molecules, demonstrating its function in cell lines and mice.

Helix: a structure-aware deep learning model for accurate prediction of A-to-I RNA editing by endogenous ADARs

Helix predicts A-to-I RNA editing by ADARs with high accuracy using a structure-aware transformer model, enabling efficient gRNA design for therapeutic applications.

Functional diversification of the cephalopod proteome by RNA-editing

This study investigates how RNA editing in cephalopods alters protein function through proteomics and biochemical assays, revealing its impact on protein stability, localization, and interactions.

Widespread remodeling of the RNA editome underlies transcriptional and clinical heterogeneity in pediatric acute lymphoblastic leukemia

The paper identifies widespread RNA editing changes in pediatric ALL, linking them to clinical outcomes and potential therapeutic targets.

A stochastic RNA editing process targets a select number of sites in individual Drosophila glutamatergic motoneurons

The paper identifies RNA editing sites in Drosophila glutamatergic motoneurons, revealing stochastic editing patterns and cell-type specific edits that may influence synaptic function.

Trisomy 21 Drives ADARB1 Overexpression and Premature RNA Recoding in the Developing Fetal Brain

This study identifies ADARB1 overexpression in T21 fetal brain due to trisomy 21, leading to increased A-to-I RNA editing and altered GRIA gene recoding.

Functional Impact of CYFIP2 RNA Editing on Actin Regulation, Axon Growth, and Spinogenesis

This study investigates the functional impact of CYFIP2 RNA editing on actin dynamics, axon growth, and spinogenesis in neural cells using CRISPR-Cas9 and shRNA technologies.

Effective in vivo RNA base editing via engineered cytidine deaminase APOBECs fused with PUF proteins

This paper develops ProAPOBECs, engineered cytidine deaminases fused with PUF proteins, for effective RNA base editing in the brain, correcting mutations and reducing autistic-like behaviors in mice.

ADARs mediate distinct RNA editing activity and gene regulation in the Caenorhabditis elegans germline

This study profiles A-to-I RNA editing activity in C. elegans germline and finds that ADARs have distinct roles compared to other tissues, with compensatory mechanisms buffering loss of function.

Epitranscriptome-wide profiling identifies RNA editing events regulated by ADAR1 that are associated with DNA repair mechanisms in human TK6 cells

ADAR1p150 RNA binding, independent of A-to-I RNA editing, buffers immunogenicity from the tonic type I IFN induced transcriptome in vivo.

This study shows ADAR1p150 suppresses immunogenicity from tonic type I IFN-induced transcripts through editing-dependent and -independent mechanisms.

Meta-analysis of genetic regulation of RNA editing in the human brain identifies new genes underlying neurological disease

The paper identifies 38,731 edQTLs in 3,554 genes through a meta-analysis of A-to-I RNA editing in the human brain and links 24 GWAS loci for neurological disorders to edQTLs.

Illuminating the Role of A-to-I Editing in Gastric Cancer using EndoVIA 2.0

This paper introduces EndoVIA 2.0, a method for spatial detection of A-to-I RNA editing in FFPE tissues, showing its utility in gastric and other cancers.