Advanced Fluorescence Microscopy Techniques

Microscale visualization of cellular features in adult macaque visual cortex

The paper visualizes dense distributions of immunolabeled proteins in adult macaque visual cortex using expansion microscopy and light sheet imaging, enabling multimodal atlas creation.

Detecting rapid pan-cortical voltage dynamics in vivo with a brighter and faster voltage indicator

This paper develops a brighter and faster voltage indicator, JEDI-1P, enabling single-trial widefield voltage imaging in awake mice.

Open top multi sample dual view light sheet microscope for live imaging of large multicellular systems

The paper introduces a dual-view light sheet microscope for live imaging of large multicellular systems at single-cell resolution and demonstrates its use on intestinal organoids and gastruloids.

LanTERN: a fluorescent sensor that specifically binds lanthanides

The paper introduces LanTERN, a fluorescent sensor engineered to bind lanthanides with high specificity and fluorescence response.

maskNMF: A denoise-sparsen-detect approach for extracting neural signals from dense imaging data

maskNMF improves demixing of dense calcium imaging data by denoising, sparsening, and using constrained non-negative matrix factorization to accurately recover neural activity.

2-photon imaging of fluorescent proteins in living swine

This paper develops techniques for high-resolution 2-photon imaging of fluorescent proteins in living swine brains, addressing challenges in motion artifact and large animal imaging.

Interrogation of single-neuron functional connectivity in the cortex and hippocampus via fast cross-layer all-optical physiology

This paper introduces a cross-layer all-optical physiology system for mapping and modifying inter-layer neuronal connectivity in live mice.

TWISP: A Transgenic Worm for Interrogating Signal Propagation in C. elegans

The paper introduces TWISP, a transgenic C. elegans strain that enables optical measurements of signal propagation at brain scale and cellular resolution.

A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light

The paper introduces WHaloCaMP, a near-infrared calcium indicator, and demonstrates its use in imaging calcium responses in various organisms.

Experimental Parameters-Based Monte-Carlo Simulation of Single-Molecule Localization Microscopy of Nuclear Pore Complex to Evaluate Clustering Algorithms

The paper evaluates clustering algorithms for nuclear pore complex analysis using synthetic SMLM data generated by Monte-Carlo simulation.

A novel polycistronic method tailored for engineering split GECIs

The paper demonstrates a method using stop-codons to enable polycistronic expression in eukaryotic cells, leading to the development of split-GECIs for monitoring cellular processes.

A Versatile and Open Source One- and Two-Photon Light-Sheet Microscope Design

This paper presents an open-source design for a one- and two-photon light-sheet microscope that reduces cost and complexity while improving accessibility.

Saturated reconstruction of living brain tissue

This paper introduces a method for saturated reconstruction of living brain tissue using advanced imaging and deep learning to achieve high-resolution, volumetric analysis of neural structures over time.

Real-time targeted illumination in widefield microscopy achieves confocal quality neuronal images

This paper introduces a targeted illumination technique for widefield microscopy to improve neuronal fiber imaging by reducing background noise and enhancing signal visibility.

A versatile Synaptotagmin-1 nanobody provides perturbation-free live synaptic imaging and low linkage-error in super-resolution microscopy

This paper introduces a nanobody that enables non-invasive live synaptic imaging and improves super-resolution microscopy with minimal physiological impact.

A 40 MHz femtosecond laser repetition frequency divider for enhanced two-photon imaging

This paper presents a 40 MHz femtosecond laser repetition frequency divider that improves two-photon imaging quality in cortical tissue.

A positively tuned voltage indicator reveals electrical correlates of calcium activity in the brain

This paper introduces positively tuned GEVIs that enhance spike detection in vivo with standard imaging systems, offering higher temporal resolution than calcium indicators.

Two-color coincidence single-molecule pull-down for the specific detection of disease-associated protein aggregates

This paper introduces a method to specifically detect disease-associated protein aggregates using two-color coincidence single-molecule pull-down, demonstrating its effectiveness with alpha-synuclein in human biofluids.

Monitoring norepinephrine release in vivo using next-generation GRABNE sensors

This paper develops improved GRABNE sensors to monitor norepinephrine release in vivo with high sensitivity and uses them to study NE dynamics in mice.

Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina

The paper develops an iterative immunostaining method combined with expansion microscopy and image processing to enhance super-resolution imaging of nuclear lamina structures.

Diffusion model of delipidation in biological sample clearing

The paper develops a diffusion model to explain delipidation in biological sample clearing using detergents and organic solvents, showing that certain organic solvents can achieve faster clearing than detergents.

Precise and versatile microplate reader-based analyses of biosensor signals from arrayed microbial colonies

This study demonstrates the use of microplate readers to analyze biosensor signals from microbial colonies, showing improved sensitivity and dynamic range for various fluorescent reporters.

High-throughput confocal airy beam oblique light-sheet tomography of brain-wide imaging at single-cell resolution

Dual-color GRAB sensors for monitoring spatiotemporal serotonin release in vivo

This paper develops dual-color GRAB sensors to monitor spatiotemporal serotonin release in vivo, revealing uniform and gradient release patterns and seizure-induced waves.

CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue

The paper introduces hCLARITY, a clearing method that improves the sensitivity and specificity of fluorescence immunostaining in archived human brain tissue for high-resolution imaging.

An inducible tricolor reporter mouse for simultaneous imaging of lysosomes, mitochondria and microtubules

The paper introduces an inducible tricolor reporter mouse for simultaneous imaging of lysosomes, mitochondria, and microtubules in native tissues at single-cell resolution.

Metabolic FRET sensors in intact organs: Applying spectral unmixing to acquire reliable signals

The paper develops an image processing method to extract reliable FRET signals from metabolic sensors in intact Drosophila organs, overcoming autofluorescence interference.

Cross-modality supervised image restoration enables nanoscale tracking of synaptic plasticity in living mice

The paper develops a deep learning image restoration method to enhance in vivo synaptic imaging resolution using ex vivo super-resolution techniques, enabling tracking of synaptic plasticity in living mice.

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

This paper develops improved glutamate indicators with faster activation kinetics and better synaptic localization for imaging synaptic transmission in neurons.

PhAT: A flexible open-source GUI-driven toolkit for photometry analysis

PhAT provides a GUI-driven open-source toolkit for photometry analysis, offering signal normalization, event alignment, fluorescence comparison, and modular development.