Extracellular vesicles in disease

Dysregulation of extracellular vesicle protein cargo in female ME/CFS cases and sedentary controls in response to maximal exercise

This study examines changes in extracellular vesicle protein cargo in ME/CFS patients and sedentary controls after maximal exercise, finding distinct proteomic responses linked to symptom severity.

Stimulative piezoelectric nanofibrous scaffolds for enhanced small extracellular vesicle production in 3D cultures

This paper develops a 3D culture platform using piezoelectric nanofibrous scaffolds with acoustic stimulation to significantly enhance small extracellular vesicle production.

Proteome encoded determinants of protein sorting into extracellular vesicles

This study predicts protein association with extracellular vesicles using sequence-based models and identifies palmitoylation as a key determinant.

Novel method for collecting hippocampal interstitial fluid extracellular vesicles (EV-ISF) reveals sex-dependent changes in microglial EV proteome in response to Aβ pathology

The paper develops a method to collect hippocampal interstitial fluid extracellular vesicles (EVISF) and finds sex-dependent changes in microglial EV proteome related to Aβ pathology.

Human cerebrospinal fluid single exosomes in Parkinson and Alzheimer diseases

The paper analyzes single exosomes in CSF from Alzheimer's and Parkinson's patients and controls, finding reduced ApoE+ exosomes in Alzheimer's patients.

Lipidome profiling of neutrophil-derived extracellular vesicles unveils their contribution to the ensemble of synovial fluid-derived extracellular vesicles during joint inflammation

The paper profiles lipidomes of neutrophil-derived extracellular vesicles in joint inflammation and finds species-specific lipid changes linked to inflammatory conditions.

Neuronal exosomes transport a miRNA/RISC cargo to preserve germline stem cell integrity during energy stress

Neurons release miRNA/Argonaute-containing exosomes to establish germline stem cell quiescence in response to energy stress.

Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles

The paper identifies state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles using proteomic and transcriptomic analyses.

Assessment of extracellular vesicle protein cargo as neurodegenerative disease biomarkers

This study develops methods to measure proteins inside extracellular vesicles and applies them to assess neurodegenerative disease biomarkers in plasma.

Exosomes promote axon outgrowth by engaging the Wnt-Planar Cell Polarity pathway

This study shows fibroblast-derived exosomes promote axon outgrowth in neurons via the Wnt-PCP pathway, while neuron- or astrocyte-derived exosomes do not or inhibit this process.

Assessment of technical and clinical utility of a bead-based flow cytometry platform for multiparametric phenotyping of CNS-derived extracellular vesicles

This study evaluates a bead-based flow cytometry platform for detecting CNS-derived extracellular vesicles and identifying disease-specific markers in clinical samples.

Multi-omic temporal landscape of plasma and synovial fluid-derived extracellular vesicles using an experimental model of equine osteoarthritis

The paper profiles extracellular vesicle cargo in plasma and synovial fluid of an equine osteoarthritis model using multi-omic approaches and identifies time-dependent changes in protein and RNA content.

A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes

This paper develops a machine learning method to identify cancer exosomes using a proteomic signature, achieving high accuracy across multiple biological fluids.

Profiling plasma extracellular vesicle-derived microRNAs for noninvasive diagnosis of alpha-1 antitrypsin deficiency associated liver disease

This study identifies miRNA biomarkers in plasma extracellular vesicles for noninvasive detection of AATD liver disease, achieving an AUC of 0.751 with a four-miRNA model.

Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles

The paper analyzes cf-mRNA in EVs and non-vesicular carriers from cancer patients and healthy donors, finding EVs selectively enrich cf-mRNA associated with specific biological pathways.

Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation

This study identifies an astroglial exosome pathway that promotes axon growth and dendritic spine formation in cortical pyramidal neurons, with ApoE inhibiting this effect.

Identification of Novel Scaffold Proteins for Improved Endogenous Engineering of Extracellular Vesicles

The paper identifies 24 novel scaffold proteins with conserved EV-sorting abilities, outperforming CD63 in cargo loading and demonstrating improved EV engineering potential.

Conserved and Divergent Modulation of Calcification in Atherosclerosis and Aortic Valve Disease by Tissue Extracellular Vesicles

The paper isolates and analyzes extracellular vesicles from atherosclerotic plaques and aortic valves, identifying shared and distinct molecular pathways involved in calcification.

Design, Synthesis, and Biological Evaluation of Novel Mitochondria-targeting Exosomes

This study designs and evaluates mitochondria-targeting exosomes for treating brain tumors and neurodegenerative diseases using in vitro and in vivo tests.

Extracellular vesicle-mediated trafficking of developmental cues is altered during human brain disease

The paper investigates how extracellular vesicles (EVs) mediate developmental cues during brain development and disease, finding altered EV composition and function in neurodevelopmental disorders.

Identification of common sequence motifs shared exclusively among selectively packed exosomal pathogenic microRNAs during rickettsial infections

The paper identifies common sequence motifs shared among specific microRNAs in exosomes from rickettsia-infected cells, linking them to microvasculopathy.

Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult in vitro and in vivo

This study identifies cystatin C as a neuroprotective protein upregulated after stroke and shows that delivering it via brain-derived extracellular vesicles improves synaptic preservation in vitro and in vivo.

Neuron Enriched Exosomal MicroRNA Expression Profiles as a Marker of Early Life Alcohol Consumption

The paper examines the relationship between early life alcohol consumption and neuron-enriched exosomal miRNA expression, finding significant associations with specific miRNAs and biological functions.

An exosome-based gene delivery platform for cell-specific CRISPR/Cas9 genome editing

The paper develops a modular exosome-based platform for targeted CRISPR/Cas9 genome editing, demonstrating efficient delivery and gene editing in vitro and in vivo.

Prenatal SARS-CoV-2 infection alters postpartum human milk-derived extracellular vesicles

This study examines how prenatal SARS-CoV-2 infection affects postpartum human milk-derived extracellular vesicles (HMEVs) through multi-omics analysis of milk samples from an IMPRINT cohort.

An optimized workflow for analyzing extracellular vesicles as biomarkers in liver diseases.

This paper presents an optimized workflow for isolating and analyzing extracellular vesicles from plasma and serum in liver diseases, identifying miRNAs and cytokines as potential biomarkers.

Annexin A6 mediates calcium-dependent secretion of exosomes

The paper identifies annexin A6 as a protein required for Ca2+-dependent exosome secretion during plasma membrane repair.

ADSC-EVs modulate primary human macrophages to an anti-inflammatory phenotype in vitro

This study shows ADSC-EVs shift M0-like macrophages to a less inflammatory state in vitro, without affecting M2-like macrophages.

Towards a human brain EV atlas: Characteristics of EVs from different brain regions, including small RNA and protein profiles

The paper characterizes extracellular vesicles (EVs) from different brain regions, revealing regional differences in EV yield, surface markers, and RNA content.

Vertical transmission of maternal DNA through extracellular vesicles associates with altered embryo bioenergetics during the periconception period

The paper investigates DNA transfer via extracellular vesicles from maternal endometrium to embryos, showing altered embryo bioenergetics.