Pluripotent Stem Cells Research

Rapid and efficient oligodendrocyte differentiation from human pluripotent stem cells via dual inhibition of BMP and Notch signaling

This paper presents a protocol to rapidly generate human oligodendrocytes from pluripotent stem cells using BMP and Notch signaling inhibition, achieving high purity and functional myelination.

Organoid-like Functional Adrenal Gland Cortex Derived From Human Pluripotent Stem Cells

This paper describes a 2D differentiation strategy from human pluripotent stem cells that generates all adrenal cortex cell types, enabling studies of adrenal biology, disease, and drug discovery.

Confocal Raman Microscopy-Guided Optimization of Early Otic Differentiation from Human Pluripotent Stem Cells

The paper optimizes BMP4 concentrations for otic differentiation from hiPSCs using Raman microscopy to monitor molecular changes and improve reproducibility.

WNT signaling in human pluripotent stem cells promotes HDAC2-dependent epigenetic programs and development of retinoic acid-responsive mesoderm

This study shows that WNT signaling in hPSCs promotes mesoderm development through HDAC2-dependent epigenetic programs and RA responsiveness, enhancing T cell generation.

Stage-adaptive integration of polydopamine promotes hPSC-derived alveolar organoids differentiation and maturation

This study shows that stage-adaptive integration of polydopamine coatings and nanoparticles enhances the maturation and secretory function of hPSC-derived alveolar organoids.

Engineering functional ventral midbrain dopaminergic neurons in human organoids through WNT modulation and bioreactor culture

This paper develops a method to generate human midbrain organoids enriched in SNpc-like dopaminergic neurons using WNT modulation and bioreactor culture, showing improved functionality and vulnerability to Parkinson's disease pathology.

iSCORE-PD: an isogenic stem cell collection to research Parkinson Disease

The paper generates a collection of 65 isogenic human stem cell lines with PD-associated mutations to study Parkinson's disease, revealing that most genetic variation comes from cell culture rather than genome editing.

Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions

This study develops a serum-free method for efficient differentiation of human pluripotent stem cells into cardiac lineages and evaluates engineered heart tissue fabrication.

Mtf2 Safeguards Naïve Pluripotency by Restricting Trophectoderm Competence

This study identifies Mtf2 as an epigenetic barrier that maintains naive pluripotency by restricting trophectoderm competence in embryonic stem cells.

Immunological responses to hydrogel-aided induced pluripotent stem cell-derived dopaminergic progenitor transplants in immunodeficient versus cyclosporine immunosuppressed rats.

This study compares immune responses to dopaminergic progenitor transplants in immunodeficient and cyclosporine-immunosuppressed rats, finding that hydrogel benefits depend on immunosuppression adequacy.

Scalable Hypothalamic Arcuate Neuron Differentiation from Human Pluripotent Stem Cells Suitable for Modeling Metabolic and Reproductive Disorders

This paper describes a scalable method to differentiate human pluripotent stem cells into hypothalamic arcuate neurons, which can model metabolic and reproductive disorders.

Establishment of a healthy control iPSC line from an Eastern Indian donor as a population specific resource for disease modelling

The paper establishes and validates an iPSC line from an Eastern Indian donor, providing a population-specific control resource for disease modelling.

Dissecting the sources of variation in neuronally differentiated iPSC lines through multi-omics analysis

The paper identifies major sources of variability in iPSC-derived neurons and shows how correcting these improves disease signature detection.

Chromosome X Dosage Modulates Development of Aneuploidy in Genetically Diverse Mouse Embryonic Stem Cells

The paper identifies that X chromosome dosage influences aneuploidy development in mouse embryonic stem cells, with XO and XY lines showing higher aneuploidy rates than XX lines.

β-Catenin Drives Apical-Basal Polarization to facilitate TE lineage commitment In Vitro and In Vivo

This study shows β-catenin drives apical-basal polarization and TE lineage commitment in mouse embryos in vitro and in vivo.

Lineage-selective suicide gene system enables post-engraftment editing of cell therapy composition

The paper introduces NeuroGuard, a suicide gene system that selectively eliminates non-neuronal cells after transplantation, enhancing the safety and predictability of cell therapies.

iAstrocytes model cytokine influences on complement expression and neuronal network synchronization.

The paper develops a method to generate functional induced astrocytes (iAstrocytes) from human pluripotent stem cells and shows their role in promoting synchronized neuronal network activity.

Pcbp1 orchestrates amino acid metabolism burst during the naive-to-primed pluripotency transition

This study identifies Pcbp1's role in enhancing amino acid metabolism during the naive-to-primed pluripotency transition, linking its deficiency to embryo growth arrest and reduced protein synthesis.

Netrin-1 drives cell-type-specific plasticity of human dopaminergic neurons during circuit integration in a Parkinsonian model

This study identifies Netrin-1 as a DA-specific cue that enhances graft integration and functional recovery in Parkinsonian models without off-target effects.

Mitochondria Clearance Enables Macrophage-Driven Maturation of iPSC-Derived Cardiomyocyte Metabolism

This study shows that adding macrophages to cardiomyocytes improves their metabolic maturity through mitochondrial clearance and mitophagy.

Human hindbrain organoids with segment-specific 5-HT neurons for motor modulation

This study generates human hindbrain organoids with segment-specific 5-HT neurons to model serotonergic development and motor modulation, revealing DISC1 deficiency disrupts serotonergic development and motor function.

ZFHX4 is necessary for dopaminergic neuron differentiation and controls cell cycle by regulating LIN28A

This study identifies ZFHX4 as a key regulator of midbrain dopaminergic neuron differentiation by controlling cell cycle progression through the LIN28A-miR-9 axis.

Spatiotemporal remodeling of cytoskeletal and junction networks during somatic cell reprogramming

This study maps spatiotemporal changes in cytoskeletal and junctional protein interactions during somatic cell reprogramming using time-resolved proximity proteomics.

A robust, reproducible, accessible and scalable protocol for generating three-dimensional human gastruloids

This paper presents a simplified, robust protocol for generating human gastruloids with high elongation efficiency and reproducibility across multiple stem cell lines.

Human club cells derived from pluripotent stem cells reveal new insights into epithelial lineage plasticity through structural and functional validation

This study generates human club cells from pluripotent stem cells, revealing epithelial plasticity and new differentiation trajectories.

Genetics of growth rate in induced pluripotent stem cells

The paper identifies genetic factors influencing iPSC growth rates, finding rare variants in WDR54 and a polygenic background explaining most variance.

EIF3H Sustains Translational Programs Essential for Proliferation in Human Primed Pluripotency

This study identifies EIF3H as essential for human primed pluripotency by regulating translation of metallothionein genes, impacting proliferation and differentiation.

Direct reprogramming of human somatic cells into neuron-like cells involves a transition through a transient intermediate state.

This study shows that direct reprogramming of human somatic cells into neurons involves a transient intermediate state, with cells exhibiting neuronal markers and functional properties.

Generation of functional vasculature from engraftable human pluripotent stem cell-derived progenitors

This paper generates engraftable vascular progenitors from human pluripotent stem cells that form functional vessels and improve perfusion in ischemic tissue.

Development and characterisation of an optimised in vitro differentiation protocol for deriving hepatocyte-like cells from mouse embryonic stem cells

This study develops and evaluates two cytokine-based protocols to generate functional hepatocyte-like cells from mouse embryonic stem cells, with the advanced cocktail producing more mature cells.