Heat shock proteins research

Identification of subfunctionalized aggregate-remodeling J-domain proteins in plants

The paper identifies subfunctionalized J-domain proteins in Arabidopsis thaliana that remodel protein aggregates, showing distinct stress responses and interactions with Hsp101.

Distinct chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4

The paper identifies a DNAJB4 variant causing chaperonopathy in skeletal muscle, leading to muscle weakness and specific pathological features in type 1 fibers.

Structural and dynamical basis for the interaction of HSP70-EEVD with JDP Sis1

This study uses NMR to show that the EEVD motif of HSP70 transiently binds multiple sites on Sis1, facilitating HSP70 interaction and client protein release.

Translational reprogramming in response to accumulating stressors ensures critical threshold levels of Hsp90 for mammalian life

The paper shows that mammalian cells can maintain critical Hsp90 levels through translational reprogramming in response to stress, ensuring viability despite genetic loss of Hsp90 alleles.

Genome-wide identification and characterization of the HSP gene superfamily in apple snails (Gastropoda: Ampullariidae) and expression analysis under temperature stress

The paper identifies and characterizes HSP gene superfamily members in apple snails and analyzes their expression under temperature stress.

The heat shock protein LarA activates the Lon protease at the onset of proteotoxic stress.

The paper identifies LarA as a regulator of Lon protease activation during proteotoxic stress, showing its role in modulating proteolysis through a specific C-terminal sequence.

Chaperones Hsc70 and Hsp70 Play Distinct Roles in Bocaparvovirus Minute Virus of Canines Infection

This study identifies Hsc70 and Hsp70 as cellular proteins interacting with MVC viral proteins and shows their distinct roles in MVC infection, suggesting Hsp70 as a potential therapeutic target.

Chaperonin activity of Plasmodium prefoldin complex is essential to guard proteotoxic stress response and presents a new target for drug discovery

This study identifies the Plasmodium prefoldin complex as essential for proteotoxic stress response and demonstrates that biperiden inhibits its function, offering a new drug target for malaria.

The ribosome quality control factors Asc1 and Hel2 regulate the expression of HSP70 during heat shock and recovery

This study identifies Asc1p and Hel2p as RQC factors that regulate HSP70 mRNA translation and degradation during heat stress and recovery in yeast.

ATP hydrolysis tunes specificity of a AAA+ protease

The paper shows that ATP hydrolysis rates alter substrate specificity of the AAA+ protease ClpX, affecting its ability to degrade different substrates.

A Comprehensive Enumeration of the Human Proteostasis Network. 1. Components of Translation, Protein Folding, and Organelle-Specific Systems

The paper defines and annotates 959 genes comprising the human proteostasis network, focusing on translation, protein folding, and organelle-specific systems.

Chaperone Requirements for De Novo Folding of Saccharomyces cerevisiae Septins

The paper shows that budding yeast septins require chaperone assistance for native folding and complex assembly, with Cdc12 being highly dependent on chaperonins.

The C-terminal domain of Hsp70 is responsible for paralog-specific regulation of ribonucleotide reductase

The paper shows that the C-terminal domain of Hsp70 paralogs Ssa3 and Ssa4 specifically regulates ribonucleotide reductase, affecting DNA damage resistance and RNR subunit stability.

Transcellular chaperone signaling is an intercellular stress-response distinct from the HSF-1 mediated HSR

The paper identifies transcellular chaperone signaling (TCS) as a gut-to-muscle stress response in C. elegans that bypasses HSF-1-mediated heat stress resistance.

Structure and Mechanism of a Structural and Functional Insights into the Action Mode of A Mitochondrial AAA+ DisaggregaseCLPB

This study reveals the structural and functional mechanism of CLPB, a mitochondrial AAA+ disaggregase, showing its unique assembly and substrate translocation properties.

A novel fluorescent multi-domain protein construct reveals the individual steps of the unfoldase action of Hsp70

This study uses a fluorescent reporter substrate to elucidate the stepwise mechanism of Hsp70 unfoldase action in converting aggregates into native proteins.

Differential roles for DNAJ isoforms in HTT-polyQ and mutant FUS aggregation modulation revealed by chaperone network screens

Targeting DNA topoisomerases or checkpoint kinases results in an overload of chaperone systems, triggering aggregation of a metastable subproteome

The paper shows that targeting ATM, ATR, or DNA topoisomerases causes widespread protein aggregation through chaperone system overload, similar to proteotoxic stress and proteinopathies.

Cryo-EM structure of hexameric yeast Lon (PIM1) highlights importance of conserved structural elements

The paper determines the cryo-EM structure of yeast Lon protease (PIM1) and shows it forms a hexamer similar to bacterial and human Lon proteases, with unique structural features and a dispensable yeast-specific insertion.

HSFs drive stress type-specific transcription of genes and enhancers

This study shows HSF1 and HSF2 regulate gene and enhancer transcription differently under oxidative stress and heat shock by controlling RNA polymerase II pause-release.

Mutations in conserved residues of the myosin chaperone UNC-45 result in both reduced stability and chaperoning activity

The paper investigates how mutations in UNC-45/UNC-45B affect myosin chaperone activity and protein stability in C. elegans, finding reduced chaperone activity and protein levels in most mutants.

A novel multifunctional role for Hsp70 in binding post-translational modifications on clients

This paper uses cross-linking mass spectrometry to characterize Hsp70 interactions in budding yeast, revealing self-association, multi-point client interactions, and novel PTMs critical for client function.

Novel proteostasis reporter mouse reveals different effects of cytoplasmic and nuclear aggregates on protein quality control in neurons

The paper develops a mouse model with a proteostasis reporter to study how cytoplasmic and nuclear protein aggregates affect neuronal protein quality control in aging and disease.

The R2TP chaperone assembles cellular machineries in intestinal CBC stem cells and progenitors

This study investigates the role of the R2TP chaperone in intestinal stem and progenitor cells, showing its critical function in proliferation and its association with poor colorectal cancer prognosis.

McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation

This study elucidates how McsB, a bacterial kinase, regulates protein degradation by switching between open and closed oligomer forms in response to stress.

Proteome plasticity in response to persistent environmental change

This study shows that budding yeast adapts to long-term high temperature by reducing temperature-sensitive proteins, relocalizing the proteome, and altering protein conformations, which may confer new functions.

Selective functional inhibition of a tumor-derived p53 mutant by cytosolic chaperones identified using split-YFP in budding yeast

The paper identifies cytosolic chaperone interactions with a tumor-derived p53 mutant using split-YFP in budding yeast, showing temperature-sensitive nuclear exclusion and dominant-negative effects.

Genome-wide, time-sensitive interrogation of the heat shock response under diverse stressors via ReporterSeq

The paper introduces ReporterSeq, a method to identify genetic regulators of the heat shock response across diverse stressors and timepoints in budding yeast.

A mathematical model of heat shock response to study the competition between protein folding and aggregation

The paper develops two mathematical models of the heat shock response to study how protein folding and aggregation compete under stress, showing bistability and the role of HSF and HSF-HSP complex in refolding proteins.

Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular aging research

The paper compares the behavior of Hsp104 fluorescent fusions in yeast, finding that fluorescent tags and fluorophores can alter molecular behavior in protein quality control and aging research.