Barrier Structure and Function Studies

HOMOCYSTEINE POTENTIATES AMYLOID β-INDUCED CEREBRAL ENDOTHELIAL CELL APOPTOSIS, BLOOD BRAIN BARRIER DYSFUNCTION AND ANGIOGENIC IMPAIRMENT

This study shows that homocysteine exacerbates amyloid β-induced cerebral endothelial cell apoptosis, BBB dysfunction, and angiogenic impairment through shared molecular mechanisms.

Unique features of the arterial Blood-Brain Barrier

The paper investigates the barrier properties of CNS arterial walls in mice and humans, finding that permeability occurs through arteriolar walls via caveolae vesicles, distinct from classical endothelial barriers.

Molecular signature and functional properties of human pluripotent stem cell-derived brain pericytes

The paper characterizes human iPSC-derived brain pericytes, showing they closely resemble primary pericytes in protein composition and function, and demonstrate therapeutic potential in neurological disorders.

The development of brain pericytes requires expression of the transcription factor nkx3.1 in intermediate precursors

This study identifies nkx3.1-expressing precursors in zebrafish that differentiate into brain pericytes and shows their regulation by cxcl12b-Cxcr4 signaling.

Endothelial Cells are Heterogeneous in Different Brain Regions and are Dramatically Altered in Alzheimer's Disease

The paper analyzes endothelial cell heterogeneity across brain regions in Alzheimer's disease using single nucleus RNAseq, revealing region-specific gene expression changes and disease-related transcriptomic alterations.

Local angiogenic interplay of Vegfc/d and Vegfa drives brain region-specific development of fenestrated capillaries

The paper identifies a core angiogenic mechanism involving Vegfc/d and Vegfa that drives region-specific development of fenestrated capillaries in the brain.

Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation.

This study identifies Hmgb1 in astrocytes as critical for postnatal astrocyte morphogenesis and cerebrovascular maturation, affecting endothelial ultrastructure and neurovascular coupling in adult mice.

Enhanced in Vivo Blood Brain Barrier Transcytosis of Macromolecular Cargo Using an Engineered pH-sensitive Mouse Transferrin Receptor Binding Nanobody

This paper develops a pH-sensitive nanobody to enhance blood brain barrier transcytosis of macromolecular cargo in mice, showing improved retention and distribution of engineered constructs.

The neurovascular unit of capillary blood vessels in the rat nervous system. A rapid-Golgi electron microscopic study.

The paper describes a pericapillary organ in the rat nervous system composed of tripartite synapses and astrocytic endfeet, suggesting a mechanoreceptor role and presynaptic modulation by Piezo 1 mRNA.

Leakage beyond the primary infarction: A temporal analysis of cerebrovascular dysregulation at sites of hippocampal secondary neurodegeneration following cortical photothrombotic stroke

This study investigates cerebrovascular changes in the hippocampus after cortical stroke, showing sustained vascular leakage and structural remodeling beyond the primary infarct site.

Systemic inflammation modulates the levels of EBI2 and 7α,25-OHC synthesizing (CH25H, CYP7B1) and degrading (HSD3B7) enzymes in brain microvascular cells

The paper investigates how systemic inflammation affects the expression of enzymes involved in 7α,25-OHC synthesis and degradation in brain microvascular cells, revealing species-specific differences between mice and humans.

Circulating exosomes from Alzheimer's disease suppress VE-cadherin expression and induce barrier dysfunction in recipient brain microvascular endothelial cell

This study shows that Alzheimer's disease circulating exosomes reduce VE-cadherin expression and impair brain endothelial barrier function through RNA cargo-dependent mechanisms.

Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a NO-CCL2-CCR2 pathway

This study identifies Slc4a4's role in maintaining blood-brain barrier integrity through the NO-CCL2-CCR2 pathway in astrocytes, showing its importance in both healthy and stroke conditions.

Aging reduces calreticulin expression and alters spontaneous calcium signals in astrocytic endfeet of the mouse dorsolateral striatum

This study shows aging reduces calreticulin in astrocytic endfeet and changes calcium signaling dynamics in the mouse dorsolateral striatum.

Microglia associations with brain pericytes and the vasculature are reduced in Alzheimer's disease

The paper identifies a subpopulation of microglia associated with pericytes in the brain and shows this population is reduced in Alzheimer's disease in the superior frontal gyrus.

SorCS2 modulates neurovascular coupling via glutamatergic and calcium signaling in astrocytes

This study shows that SorCS2 is critical for neurovascular coupling in mice through its role in glutamatergic and calcium signaling in astrocytes.

Age-associated changes in endothelial transcriptome and chromatin landscape correlate with elevated risk of hemorrhage

The paper investigates age-related changes in endothelial cells of mice, identifying gene and chromatin alterations linked to increased intracerebral hemorrhage risk.

Zika virus E protein alters blood-brain barrier by modulating brain microvascular endothelial cell and astrocyte functions.

This study investigates how ZIKV E protein affects BBB integrity by altering hBMECs and astrocytes, leading to increased permeability and inflammation.

High and low permeability of human pluripotent stem cell-derived Blood Brain barrier models depend on epithelial or endothelial features

This study compares two hiPSC-derived BBB models, finding that EC-like cells produce high permeability models while EpC-like cells show better barrier properties.

Blood-brain barrier dysfunction promotes astrocyte senescence through albumin-induced TGFβ signaling activation

Endothelial structure contributes to heterogeneity in brain capillary diameter

The paper examines how endothelial cell structure influences capillary diameter heterogeneity in mouse brain microvasculature using electron microscopy data.

The Impact of Pathogenic and Artificial Mutations on Claudin-5 Selectivity from Molecular Dynamics Simulations

The paper uses molecular dynamics simulations to study how pathogenic and artificial mutations affect ion selectivity in Claudin-5 paracellular pores, identifying structural models that align with experimental observations.

Ultrastructure of Precapillary Sphincters and the Neurovascular Unit

This study uses 3D electron microscopy to examine the neurovascular unit, revealing new structural roles for precapillary sphincters, collagen, and astrocytic endfeet in neurovascular coupling.

The impact of alpha-synuclein aggregates on blood-brain barrier integrity in the presence of neurovascular unit cells

This study investigates how alpha-synuclein aggregates affect blood-brain barrier integrity in vitro using co-cultures of brain endothelial cells with neurovascular unit components.

Establishing And Maintaining The Blood-Brain Barrier: Epigenetic And Signaling Determinants.

The paper identifies epigenetic and signaling factors that regulate BBB formation and maintenance in CNS endothelial cells.

Amyloid-beta peptides 40 and 42 employ distinct molecular pathways for cell entry and intracellular transit at the BBB endothelium

This study identifies distinct endocytic pathways for Aβ40 and Aβ42 in BBB endothelial cells, showing clathrin-mediated uptake for Aβ40 and caveolae-mediated uptake for Aβ42, with differential intracellular trafficking outcomes.

Selection of single domain anti-transferrin receptor antibodies for blood-brain barrier transcytosis using a neurotensin based assay and histological assessment of target engagement in a mouse model of Alzheimer's related amyloid-beta pathology

The paper develops anti-mTfR VHHs for BBB transcytosis and evaluates their hypothermic effects and target engagement in a mouse model, finding no direct correlation between hypothermia and plaque labeling.

REDUCED FOLATE CARRIER 1 IS PRESENT IN RETINAL MICROVESSELS AND CRUCIAL FOR THE INNER BLOOD RETINAL BARRIER INTEGRITY

This study shows RFC1 is present in retinal microvessels and regulates inner blood-retinal barrier integrity by affecting tight junction proteins and collagen-4.

Mitochondrial complex I controls blood brain barrier permeability

This study shows that mitochondrial complex I regulates blood brain barrier permeability by controlling energy production and metabolic flux in endothelial cells.

Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport

The paper identifies 17β-estradiol and CAT-1 as regulators of leptin transport across an iPSC-derived blood-brain barrier model.