Barrier Structure and Function Studies

Energy supply per neuron is constrained by capillary density in the mouse brain

The paper examines capillary density in the mouse brain and finds it is mostly homogeneous, with energy supply per neuron decreasing as neuronal density increases.

DISRUPTED CEREBRAL PERI-MICROVASCULAR GLYCOGEN PROMOTES CAPILLARY CONSTRICTIONS AND AGGRAVATES ISCHEMIA IN MICE

This study shows that disrupting glycogen utilization in mice causes microvascular constrictions and worsens ischemia outcomes.

Computational Assessment of Different Structural Models for Claudin-5 Complexes in Blood-Brain-Barrier Tight-Junctions

This study compares two structural models of Claudin-5 complexes in BBB tight junctions using molecular dynamics and free energy calculations to assess their ionic transport properties.

Axo-vascular coupling mediated by oligodendrocytes

The paper demonstrates that oligodendrocytes mediate axo-vascular coupling in the mouse optic nerve by regulating vascular dilation in response to axonal activity.

Formation and function of the meninges arachnoid barrier around the developing brain

The paper identifies Wnt-β-catenin signaling repression as key to arachnoid barrier cell specification in mouse meninges and shows its role in preventing pathogen entry during development.

A host-gut microbial co-metabolite of aromatic amino acids, p-cresol glucuronide, promotes blood-brain barrier integrity in vivo

This study shows that p-cresol glucuronide, a host-gut microbial co-metabolite, reduces blood-brain barrier permeability and modulates brain transcriptome expression in mice.

Pericyte remodeling is deficient in the aged brain and contributes to impaired capillary flow and structure

This study shows that pericyte loss in aged brains leads to capillary dilation, flow heterogeneity, and structural disruption, highlighting pericyte remodeling as a therapeutic target.

A Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Dysregulation of the Neurovascular Unit Relevant to Brain Inflammation

This study uses a SARS-CoV-2 biomimetic to investigate how BBB dysregulation may lead to neuroinflammation without direct viral infection.

Two-photon microscopy in vivo reveals brain vessel type-specific loss of glycocalyx caused by apoM/S1P signaling impairment.

The paper uses two-photon microscopy to show that S1P signaling impairment causes glycocalyx loss in arterioles but not other vessel types in Apom-/- mice.

Public volume electron microscopy data: An essential resource to study the brain microvasculature

The paper presents a meta-analysis of electron microscopy datasets to explore cerebrovascular structures and identifies features important for vascular function and segmentation improvements.

Electro-Metabolic Sensing Through Capillary ATP-Sensitive K+ Channels and Adenosine to Control Cerebral Blood Flow

The paper demonstrates that brain capillary endothelial cells and pericytes express functional KATP channels that regulate membrane potential and cerebral blood flow through adenosine signaling.

The general anesthetic isoflurane inhibits calcium activity in cerebrovascular endothelial cells and disrupts vascular tone

This study shows that isoflurane induces initial calcium increases then suppression in cerebrovascular endothelial cells, disrupting vascular tone during anesthesia.

Brain Capillary Pericytes are Metabolic Sentinels that Control Blood Flow through KATP Channel Activity

This study identifies capillary pericytes as metabolic sentinels that increase blood flow in response to glucose depletion through KATP channel activation.

TUNNELING NANOTUBES CONNECT BLOOD-BRAIN BARRIER CELLS: ROLE OF PERICYTES IN BARRIER PRESERVATION DURING ISCHEMIA

This study shows that tunneling nanotubes (TNTs) enable mitochondrial transfer between BBB cells, rescuing astrocytes from ischemia/reperfusion-induced apoptosis and highlighting pericytes' role in BBB preservation.

Reactive astrocytes transduce blood-brain barrier dysfunction through a TNFα-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin

Mural norrin/β-catenin signaling regulates Lama2 expression to promote neurovascular unit assembly

This study shows that Norrin/β-catenin signaling in mural cells increases Lama2 expression, which promotes neurovascular unit assembly and barrier maturation.

Single-cell multi-region dissection of brain vasculature in Alzheimer's Disease

The paper identifies cell-type-specific molecular changes in cerebrovascular cells across brain regions in Alzheimer's Disease, linking them to genetic variants and potential therapeutic targets.

Increased capillary stalling is associated with endothelial glycocalyx loss in subcortical vascular dementia

Sex-specific blood-brain barrier alterations and vascular biomarkers underlie chronic stress responses in mice and human depression.

The cellular architecture of microvessels, pericytes and neuronal cell types in organizing regional brain energy homeostasis in mice

The paper maps the spatial relationship between cerebrovasculature, pericytes, and neuronal cell types in mice to reveal how they support regional brain energy homeostasis.

A sporadic Alzheimer's blood-brain barrier model for developing ultrasound-mediated delivery of Aducanumab and anti-Tau antibodies.

This study demonstrates that focused ultrasound with microbubbles enhances the delivery of Aducanumab and anti-Tau antibodies across a human sporadic Alzheimer's disease blood-brain barrier model.

Single Nuclei Transcriptome Reveals Perturbed Brain Vascular Molecules in Alzheimer's Disease

The paper uses single nucleus RNA sequencing to analyze brain vascular cells in Alzheimer's disease, identifying molecular changes and interactions between vascular and astrocytic cells at the blood-brain barrier.

Cx43 carboxyl terminal domain determines AQP4 and Cx30 endfoot organization and blood brain barrier permeability

This study investigates how the carboxyl terminal domain of Cx43 affects astrocyte endfoot organization and BBB permeability in mice.

Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging

The paper uses in vivo two-photon imaging to distinguish perivascular fibroblasts from mural cells in mouse cerebral cortex, revealing unique structural and distributional features.

Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzyme

The paper compares brain delivery and efficacy of two TfR-targeted enzyme fusions in MPS II mice, finding the moderate-affinity ETV:IDS has better brain exposure and substrate reduction than the high-affinity IgG:IDS.

Regulation of blood-brain barrier integrity by microbiome-associated methylamines and cognition by trimethylamine N-oxide

This study shows that TMAO enhances BBB integrity and protects against inflammation, while TMA impairs BBB function and cognitive resilience in mice.

Neurovascular unit alterations in the growth restricted newborn are improved following ibuprofen treatment

This study shows ibuprofen reduces inflammation and restores neurovascular unit integrity in growth-restricted newborn piglets.

Defining the Transcriptional and Epigenetic Basis of Organotypic Endothelial Diversity in the Developing and Adult Mouse

This study identifies transcriptional and epigenetic mechanisms driving endothelial diversity across organs in mice, revealing conserved gene regulatory networks in the brain vasculature.

Wnt signaling mediates acquisition of blood-brain barrier properties in naïve endothelium derived from human pluripotent stem cells

This study shows that activating Wnt signaling in human pluripotent stem cell-derived naive endothelial progenitors promotes the acquisition of blood-brain barrier properties.

High Sucrose Diets Contribute to Brain Angiopathy with Impaired Glucose Uptake, and Psychosis-related Higher Brain Dysfunctions in Mice

This study shows that high-sucrose diets in Glo1-deficient mice cause brain angiopathy, impaired glucose uptake, and psychosis-related behaviors, with aspirin offering protection.