Congenital heart defects research

Cell-autonomous Wnt activity promotes transient re-programming and cell cycle re-entry of coronary artery endothelial cells

The paper identifies Wnt signaling as essential for coronary artery endothelial cell reprogramming and collateral artery formation in mice, with implications for cardiovascular disease.

ERK activity as a key player in determining cardiac cell fate choices

The paper shows that ERK activity influences cardiac cell fate by directing differentiation towards JCF and SHF markers over FHF-like cardiomyocytes in an in vitro system.

Longitudinal Live Imaging Derived 4D Hemodynamics and Dynamic Tissue Mechanics Across Outflow Tract Morphogenesis

The paper uses 4D CFD simulations to study hemodynamics and tissue mechanics in the cardiac outflow tract during development, revealing correlations between wall shear stress and tissue extension.

Polyploid cardiomyocytes define disease-specific transcriptional states in the mammalian heart

This study identifies polyploid cardiomyocytes as disease-specific transcriptional states in the mammalian heart and suggests targeting them for heart failure treatment.

Enhancer-mediated metabolic pre-patterning defines trabecular cardiomyocyte identity prior to morphogenesis

The paper identifies an enhancer, -6.8got2b, critical for trabecular cardiomyocyte development in zebrafish and shows its conservation and function in humans.

Patient-informed CRISPR Screen Identifies FLNB as a Novel Congenital Heart Disease and Ciliopathy Gene

The paper uses a CRISPR screen in Xenopus to identify FLNB as a novel gene associated with congenital heart disease and ciliopathies.

Harnessing glucocorticoid receptor antagonization to enhance the efficacy of cardiac regenerative growth factors and cytokines

This paper shows that blocking glucocorticoid receptors enhances the effectiveness of growth factors in promoting cardiac regeneration by restoring MAPK/ERK signaling in cardiomyocytes.

Neural crest cell derived DKK1 modulates Wnt signalling in the second heart field to orchestrate cardiac outflow tract development

The paper identifies DKK1 from neural crest cells as a regulator of Wnt signaling in the second heart field, crucial for cardiac outflow tract development and linked to congenital heart disease.

Activation of developmental transcription factors using RNA technology promotes heart repair

This study shows that inhibiting miR-200c in adult mice improves heart function and reduces fibrosis after myocardial infarction by reactivating developmental transcription factors.

Rtf1-dependent transcriptional pausing regulates cardiogenesis

This study shows that Rtf1-mediated transcriptional pausing is essential for cardiogenesis, as its disruption leads to cardiac progenitor cell defects and cardiomyocyte absence.

Exercise-Induced Epoxy-Eicosanoids Promote Cardiomyocyte Proliferation and Post-Infarction Repair via NR4A1

This study shows exercise increases 17,18-EEQ, which promotes cardiomyocyte proliferation and post-MI repair via the PKA-CREB-NR4A1 pathway.

Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia

The paper develops a workflow for isolating and profiling adult teleost cardiac cells, revealing exercise-induced hypertrophy and hyperplasia and defining proteo-transcriptomic regulatory networks.

The PIDDosome controls cardiomyocyte polyploidization during postnatal heart development

This study shows the PIDDosome restricts cardiomyocyte polyploidization during postnatal heart development through p21/Cdkn1a, independent of p53.

Distinct roles of the Lyve1 lineage in heart development

The paper identifies distinct roles of the Lyve1 lineage in heart development, showing its contribution to multiple cardiovascular cell types and demonstrating functional consequences of genetic perturbations.

Hedgehog and Bmp signaling pathways play opposing roles during establishment of the cardiac inflow tract in zebrafish

This study shows that Hedgehog signaling restricts IFT cardiomyocyte number while Bmp signaling promotes their formation in zebrafish embryos.

A disrupted compartment boundary underlies abnormal cardiac patterning and congenital heart defects

The paper identifies Tbx5 as a gene critical for forming a compartment boundary in the interventricular septum, and shows its disruption leads to congenital heart defects.

Mouse Cardiac Regeneration Database (MCAREDB): a single-cell atlas of neonatal cardiac regeneration in mice

MCAREDB integrates scRNA-seq datasets from mouse cardiac regeneration studies to enable comparative analysis of gene expression and pathways across developmental and injury contexts.

Deciphering the functional association of novel variants of BMP7 in isolated congenital heart disease by integrating in vitro and in silico approaches

The paper identifies and functionally characterizes novel BMP7 variants in congenital heart disease using in vitro and in silico methods, showing gain-of-function effects.

Deletion of the polarity protein Llgl1 impairs cardiomyocyte intercalated disc integrity and permits cell cycle activity into adulthood

This study shows that deleting Llgl1 in cardiomyocytes disrupts intercalated disc integrity, causes electrical conduction defects, and allows cell cycle activity into adulthood, while improving cardiac function after injury.

AXL governs axolotl cardiac regeneration and directs mammalian cardiomyocyte dedifferentiation

This study identifies AXL as a key regulator of cardiac regeneration in axolotls and shows its role in inducing cardiomyocyte dedifferentiation in mammals without cell cycle re-entry.

Functional architecture of cardiac TF regulatory landscapes in control of mammalian heart development

The paper identifies essential cardiac enhancer modules and regulatory mechanisms governing heart development, revealing resilience in cis-regulatory architectures and the role of Hand2 upstream regulatory intervals.

Scube2 Modulates Coronary Vessel Formation during Cardiac Growth and Regeneration in Zebrafish

This study identifies Scube2 as a regulator of coronary vessel formation and heart regeneration in zebrafish, showing its role in vascular coverage, myocardial structure, and revascularization after injury.

Reduced TBX5 dosage undermines developmental control of atrial cardiomyocyte identity in a model of human atrial disease

This study shows that reduced TBX5 dosage disrupts atrial cardiomyocyte identity, leading to cellular and molecular changes associated with atrial diseases.

Spatiotemporal Atlas of Heart Development Reveals Blood-Flow-Dependent Cellular, Structural, Metabolic, and Spatial Remodeling

The paper generates a spatiotemporal atlas of heart development using flow perturbations and spatial transcriptomics to study how blood flow affects cellular, structural, and metabolic remodeling.

Functional analysis across model systems implicates ribosomal proteins in growth and proliferation defects associated with hypoplastic left heart syndrome

The paper identifies ribosomal protein genes as contributors to hypoplastic left heart syndrome through functional analysis across multiple model systems.

Epicardial Tcf21 facilitates cardiomyocyte dedifferentiation and heart regeneration in zebrafish

This study investigates the role of Tcf21 in zebrafish heart regeneration by knocking out its expression and observing reduced cardiomyocyte dedifferentiation and impaired regeneration.

MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesis

This study identifies MEF2C-dependent gene regulatory networks in specific heart segments and shows their role in heart tube morphogenesis using multi-omics data and deep learning.

Interferon signaling promotes early neutrophil recruitment after zebrafish heart injury

This study identifies IFNφ1 as a key driver of interferon signaling after zebrafish heart injury, showing its role in promoting early neutrophil recruitment but not macrophage response or neovascularization.

Transcriptional Readthrough at Atf4 Locus Suppresses Rps19bp1 and Impairs Heart Development

The paper shows that cardiac defects in ATF4 knockout mice result from unintended Rps19bp1 downregulation due to transcriptional readthrough, not loss of ATF4 function.

Hox-Meis-relayed gene regulatory transition underlies cardiopharyngeal neural crest diversification

The paper maps lineage trajectories and gene regulatory networks of cardiopharyngeal neural crest cells in mice, identifying a Hox-Meis-regulated transition associated with cardiac cushion development.