DNA Repair Mechanisms

More than just a passive brick in the wall: the nucleosome facilitates DNA polymerase β activity in linker DNA and its PARP-dependent regulation in the BER sub-pathway choice

The paper shows that nucleosomes enhance DNA polymerase β activity in linker DNA and that PARP1/PARP2 regulate this activity and BER sub-pathway choice.

Role of small non-coding RNAs in DSB repair in Arabidopsis thaliana

The paper investigates the role of small non-coding RNAs (diRNAs) in DNA double-strand break (DSB) repair in Arabidopsis thaliana and finds they are not essential for repair but may silence damaged genes post-transcriptionally.

The replication checkpoint couples fork progression to the release of transcribed genes from nuclear pores

This study shows that replication stress causes genes to detach from nuclear pores, coordinated with fork progression, and that checkpoint kinases Mec1/ATR and Rad53/CHK1 regulate this process in a stepwise manner.

Single-Cell Proteomics Reveals Heterogeneous and Bimodal Proteome Responses to DNA Damage

This study uses single-cell proteomics to show that DNA damage causes heterogeneous and bimodal proteome responses in U2 OS cells, revealing cell-to-cell variability obscured by bulk analysis.

DNA polymerase kappa supports repair-associated DNA synthesis and genome maintenance in postmitotic neurons

This study identifies DNA polymerase kappa (POLK) as essential for DNA repair and genome maintenance in postmitotic neurons, showing its role in preventing neurodegeneration and aging-related stress.

Dependency Map correlation analysis reveals WDR89 as a genome maintenance factor

The paper uses DepMap data to identify WDR89 as a genome maintenance factor through correlation analysis of genetic dependencies.

Analysis of Naturally Arising Simple Deletions in Single Human Colon Crypts with Age

The paper analyzes simple deletions in single human colon crypts across age, finding most are due to strand slippage and NHEJ, with age-related accumulation and radiation effects.

Prime editing enables high-throughput identification and characterization of selective MutSβ inhibitors

This study uses prime editing to identify and characterize MutSβ inhibitors that reduce (CAG)n repeat expansion.

SLC25A28 is a synthetic lethal vulnerability in nucleotide excision repair-deficient cancers

This study identifies SLC25A28 as a synthetic lethal target in NER-deficient cancers, showing its loss impairs cell viability and increases oxidative stress.

Real-Time Visualization of G2L4 Reverse Transcriptase in DNA Repair via Microhomology-Mediated End Joining

This study visualizes G2L4 reverse transcriptase's role in DNA repair via microhomology-mediated end joining using high-speed atomic force microscopy.

Core NHEJ Factors Maintain Synapsis Across Diverse DNA End Structures

The paper investigates how DNA end chemistry affects NHEJ synaptic complex formation and stability using single-molecule imaging in Xenopus egg extract.

The 9-1-1 complex protects ssDNA gaps in BRCA2-deficient cancer

The paper identifies the 9-1-1 complex as essential for stabilizing ssDNA gaps in BRCA2-deficient cells through an ATR-independent mechanism, highlighting its role in preventing pathological expansion and DNA damage.

The FANCM-RMI1/2 complex promotes genomic instability and PARP inhibitor sensitivity in BRCA2-deficicient cells.

This study identifies FANCM-RMI1/2 as a driver of PARP inhibitor sensitivity in BRCA2-deficient cells, revealing distinct adaptation mechanisms between BRCA1 and BRCA2 loss.

Loss of PIMREG impairs double-strand break signaling and repair

This study investigates the role of PIMREG in DNA damage response in GBM cells, finding that its loss impairs double-strand break signaling and repair.

DNA:RNA hybrid mutational landscapes reveal a common route to geneticinstability in evolving genomes

The paper identifies mutational signatures caused by DNA:RNA hybrids in yeast genomes, revealing a common mechanism of genetic instability through repair pathways.

A high-throughput screening dataset of small-molecule inhibitors across human DNA glycosylases

The paper establishes a high-throughput screening dataset of small-molecule inhibitors across nine human DNA glycosylases, identifying previously unreported inhibitors and making data publicly available.

MCM10 and SLD-2/RECQL4 jointly activate the CMG helicase during metazoan DNA replication initiation

The paper shows that MCM10 and SLD-2/RECQL4 are required for helicase activation in metazoan DNA replication, with mutations in these factors linked to human disease.

Replication-coupled chromatin assembly cooperates with ATR/CHK1 signaling to suppress DNA damage and premature MMB-dependent mitotic transcription

The paper identifies a link between replication-coupled chromatin assembly and ATR/CHK1 signaling in suppressing DNA damage and MMB-dependent mitotic transcription.

Replication protein A prevents unregulated priming and Rad51 loading on single-stranded DNA in nuclear extracts of Xenopus eggs

This study shows that RPA prevents unregulated priming and Rad51 loading on ssDNA in Xenopus egg extracts, maintaining genome stability.

The rapid delivery of RNase H variants reveals distinct classes of RNA:DNA hybrids

The paper develops iCoRD to rapidly deliver RNase H1 in live cells and shows it affects specific RNA:DNA hybrids and replication fork stability.

Interplay between flap endonuclease 1 and DNA polymerase β in long patch base excision repair: new facets in mechanism

The paper investigates the mechanism of long patch base excision repair by analyzing how FEN1 and Polβ interact through equilibrium nucleotide areas rather than a gap-translation mechanism.

RNF25 Ubiquitin E3 activity Safeguards Genome Integrity by Modulating DNA Replication, Transcription and Translation.

This study identifies RNF25 as an E3 ubiquitin ligase that maintains genome integrity by modulating DNA replication, transcription, and translation through ubiquitin-mediated clearance of key proteins.

Rad55-Rad57 and Srs2 regulate homology search onset, coordination, reach and inactivation

This study investigates how Rad55-Rad57 and Srs2 regulate homology search in yeast by controlling Rad51-ssDNA filament stability and coordination.

Dna2-intrinsic condensation regulates DNA end resection and reveals evolutionary redistribution of condensate grammar

This study shows that Dna2 forms condensates that enhance DNA end resection in yeast, with phosphorylation regulating condensate stability and function.

Pan-Cancer Genomic Scars of Alternative End Joining and Single-Strand Annealing

The paper identifies genomic scars from alternative end joining and single-strand annealing across multiple cancer types, revealing their association with HR deficiency and local genomic features.

Rac1 and CHK1 Converge on Abi-1 to Regulate DNA Repair Dependency and Treatment Response in Human Cancers

This study identifies Abi-1-S323 phosphorylation as a biomarker for DNA repair states and treatment response in cancers, showing its role in regulating radiosensitivity through Rac1 and CHK1 signaling.

Nanoscale insights into chromatin integrity molecular rearrangements upon DNA damage response

This study uses nanoscale techniques to investigate chromatin changes during DNA damage response, finding protein conformation shifts and DNA demethylation linked to repair processes.

PAXIP1-PAGR1 directs cohesin recruitment during break-induced telomere repair

The paper identifies PAXIP1-PAGR1 as essential for cohesin recruitment during break-induced telomere repair and shows their role in ALT-mediated telomere maintenance.

Timing of DNA damage checkpoint adaptation predicts post-mitotic proliferative competence

The paper shows that the duration of DNA damage checkpoint arrest in budding yeast predicts post-mitotic proliferative competence after checkpoint adaptation.

Replication Fork-Associated Checkpoint Mediator Mrc1/Claspin Acts in Arrest-Independent Double-Strand Break Survival

The paper identifies Mrc1/Claspin as a novel regulator of DSB repair independent of the Mec1 checkpoint, showing it promotes DSB survival through end-tethering and limiting resection.