DNA Repair Mechanisms

A superfamily 1 helicase translocates on double-stranded DNA

This study shows SF1 helicase Rep translocates processively on ssDNA and, unexpectedly, also on dsDNA, with high speed and processivity, challenging existing models of helicase function.

Single-molecule imaging of DNA repair and cytoplasmic rigidification in intracellular bacteria

This study uses live-cell single-molecule tracking to measure DNA repair activity in intracellular E. coli and links it to metabolic and biophysical changes during macrophage infection.

Temporal proximity proteomics reveals ANP32A roles in replication fork progression and end joining

This study identifies ANP32A's role in DNA replication and repair by analyzing its proteomic interactions during replication stress and shows its involvement in end joining but not stalled-fork protection.

An integrated genome-wide resource reveals distinct replication environments of DNA breakage in human cancer cell lines

The paper identifies replication environments associated with DNA breakage in cancer cell lines by integrating genomic and transcriptomic data.

Germline removal reprograms somatic genome maintenance towards faster, energy-efficient, high-fidelity DNA defence and repair

This study shows that removing the germline in zebrafish reprograms somatic DNA repair and stress responses, favoring high-fidelity mechanisms and altering gene expression patterns across multiple organs.

Mechanistic basis for the spatiotemporal assembly of the Bloom Syndrome complex on ultrafine DNA bridges

This study identifies how the BTRR complex assembles on ultrafine DNA bridges through SLiM recognition by RMI1-RMI2, revealing spatiotemporal regulation during DNA repair and segregation.

Helicase-deficient TFIIH causes severe disease features via persistent DNA excision without damage removal

The paper shows that helicase-deficient TFIIH causes severe disease by trapping DNA in a futile repair cycle, leading to persistent excision without lesion removal.

FANCM restrains structural genome evolution and defines a synthetic lethal dependency in BRCA1-deficient breast cancer

This study identifies FANCM as a synthetic lethal target in BRCA1-deficient breast cancer, showing it restricts structural genome instability and links replication stress to transcriptional changes.

RNAPII and XPC remodel the 3D genome for UV repair

This study shows that RNAPII and XPC remodel chromatin loops and domains during UV repair, enhancing lesion recognition and repair efficiency.

End Processing in NHEJ by Polymerase {lambda} and PNKP is coordinated during short-range synapsis

The paper determines the cryo-EM structure of Pol λ bound to the short-range synaptic complex, revealing its recruitment mechanism and coordinated activity with PNKP in NHEJ end processing.

DNA binding contributes to dominant-negative effects of a catalytically inactive RecQ4-family helicase

The paper investigates how a catalytically inactive RecQ4-family helicase causes dominant-negative effects in DNA repair by showing DNA binding contributes to toxicity.

MCM10 and RECQL4 have cooperative and redundant roles in activating the CMG helicase during the replication initiation

This study shows MCM10 and RECQL4 work together and can replace each other in activating the CMG helicase during DNA replication initiation.

DNA Damage and Repair Mechanisms in Duckweed (Spirodela polyrhiza) Under Ultraviolet-B (UV-B) Light Stress

This study examines DNA damage and repair in duckweed under UV-B light stress, showing efficient repair mechanisms and oxidative stress responses.

Nuclear Cathepsin L Remodels the Replication Machinery to Create a Therapeutic Vulnerability in Ovarian Cancer

This paper identifies nuclear cathepsin L as a regulator of replication stress in ovarian cancer, showing that its activation creates a therapeutic vulnerability through replication machinery remodeling and DNA repair inhibition.

The nuclear actin cytoskeleton supports DNA double-strand break repair via VCP-mediated extraction of the KU70/80 complex from damaged chromatin

This study identifies Myo6's role in DSB repair by interacting with KU70/80 and VCP, facilitated by F-actin, to extract the KU70/80 complex from chromatin.

Molecular basis of nick ligation in the nucleosome by DNA Ligase IIIα

This study investigates how DNA Ligase IIIα ligates nicks in nucleosomes using biochemical assays, molecular dynamics, and cryo-EM, revealing position-dependent efficiency and structural constraints.

Nuclear Myosin VI stabilises Ku-associated DNA ends during non-homologous end joining

This study identifies myosin VI as a regulator of DNA double-strand break repair, linking ATM signalling to Ku-associated DNA-end stabilisation and suggesting potential therapeutic targeting.

Cohesin promotes genomic stability by suppressing unequal sister chromatid exchange

This study shows cohesin suppresses unequal sister chromatid exchange by maintaining arm cohesion and limiting distal repeat interactions in yeast.

PGM3 inhibition rewires RUVBL2-dependent DNA repair and induces a BRCAness-like state in pancreatic cancer cells

This study shows that inhibiting PGM3 in pancreatic cancer cells disrupts DNA repair pathways, induces a BRCAness-like state, and increases sensitivity to PARP inhibitors and radiation.

The Conformation of the Complementary Strand and the Deformation of the DNA Groove upon DDB2 Binding Justifies the Different Repair Rates for Cyclobutane Pyrimidine Dimers.

This study shows that DDB2 binding causes different DNA deformations depending on the cyclobutane pyrimidine dimer type, which explains the varying repair rates observed in human cells.

Replication stress at centromeres biases the segregation of DNA damage

This study identifies a mechanism by which replication stress at centromeres leads to non-random segregation of DNA damage during mitosis, influenced by R-loops, Rad51 activity, and spindle dynamics.

A germline KDM3C polymorphism impairs DNA repair and sensitizes to chemoradiotherapy

The paper identifies a germline KDM3C polymorphism linked to impaired DNA repair and increased sensitivity to chemoradiotherapy in cancers like rectal and head and neck squamous cell carcinomas.

Persistent chromatin alterations and gene expression reprogramming follow widespread DNA damage in glioblastoma

This study shows that DNA damage in glioblastoma cells leads to long-term chromatin changes and gene expression reprogramming, affecting cellular function and tumor outcomes.

Inherited DNA damage generates multi-allelic mutations in C. elegans

The paper shows that DNA lesions in C. elegans can be inherited and generate multi-allelic mutations across generations.

DNA end-resection is stimulated by an interaction between BRCA1 exon 11 and TOPBP1

The paper identifies a DNA damage- and phospho-dependent interaction between BRCA1 exon 11 and TOPBP1, showing its role in end-resection during homologous recombination.

Repair of DNA double-strand breaks after low radiation doses in childhood cancer survivors and matched cancer-free individuals

This study compares DNA double-strand break repair efficiency at low and high damage levels between childhood cancer survivors and cancer-free individuals, finding that survivors repair low-level breaks more efficiently.

A flow cytometry-based screening platform for identifying candidate radiosensitizers targeting DNA repair

This paper develops a flow cytometry-based screening platform to identify radiosensitizers targeting DNA repair, finding compounds that enhance radiosensitivity in cancer cells.

MUTYH activity maintains telomere stability in response to chronic telomeric 8-oxoguanine damage in cancer cells

This study shows MUTYH prevents telomere shortening and genomic instability in cancer cells under chronic 8-oxoG damage by resolving BER intermediates.

Functional genomics guided multi-omics framework identifies aldehyde metabolism as a therapeutic vulnerability in Fanconi anaemia

The paper identifies aldehyde metabolism as a therapeutic target in Fanconi anemia using a multi-omics approach integrating CRISPR data and transcriptomics.

XRCC1 Enables the Efficient Local Search for DNA Damage by DNA Polymerase Beta

This study shows XRCC1 enhances DNA polymerase beta's efficiency in locating DNA damage by shifting its search strategy from 3D to 1D diffusion, improving damage recognition.