Hematopoietic Stem Cell Transplantation

Embryonic TGF-β signaling imposes persistent changes to HSPC clonality and inflammatory landscape

This study shows that inhibiting TGF-β signaling during embryonic hematopoiesis alters HSPC behavior and leads to long-term changes in immune function and clonal diversity.

Resolving hematopoietic stem versus progenitor cell potential in the mouse dorsal aorta by differential Runx1 +110 enhancer activity

The paper identifies a Runx1 enhancer that distinguishes hematopoietic stem cells (HSCs) from progenitor cells (HPCs) in the mouse dorsal aorta using enhancer-reporter models and single-cell multiome analysis.

Culture enhanced MSCs to boost haematopoietic engraftment in a model of the post-leukaemic bone marrow niche.

This study improves MSCs for HSCT by suppressing senescence and shows enhanced engraftment in an AML niche model.

Developmental Gfi1 Dynamics Define Hematopoietic Emergenceand Adult Hematopoietic Stem Cell Potency

This study tracks Gfi1 expression dynamics during hematopoietic development and identifies its role in defining EHT and HSC potency.

Clonal memory of cell division in humans diverges between healthy haematopoiesis and acute myeloid leukaemia

The paper shows clonal memory in human HSPCs persists across divisions and is disrupted in AML, with potential for therapeutic modulation.

DNA barcoding-based paired daughter cell analysis reveals division preferences of hematopoietic stem cells

The paper develops a DNA barcoding-based method to analyze HSC daughter cell pairs, revealing biased division patterns toward symmetric divisions and linking these to molecular features.

The endothelial scavenger receptor stab2 is required for proper hematopoietic stem and progenitor cell development in the fetal blood stem cell niche

This study shows that stab2 is essential for fetal HSPC development in the niche, with loss of stab2 reducing HSPC numbers and altering sub-population balances.

JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

JARID2 inhibition enhances repopulating activity of human hematopoietic stem and progenitor cells in vivo via STAT1 upregulation.

Timed STING Inhibition Mitigates Gastrointestinal GvHD While Preserving Graft-versus-Leukemia Activity After Allo-HSCT

This study shows that timed STING inhibition reduces gastrointestinal GvHD without affecting GvL in mouse models, suggesting a potential therapeutic strategy for allo-HSCT.

Donor-Targeted Anti-HLA-A2 Antibody Shows Nanogram-Level Efficacy in a Novel GVHD Mouse Model

This paper develops an HLA-A2-specific antibody that effectively reduces GVHD in a mouse model with minimal dose.

Targeting cellular senescence alleviates bone marrow aging

This study shows that clearing senescent cells with 753b improves bone marrow aging by restoring HSC function and niche balance.

Septin7 is essential in early hematopoiesis, but redundant at later stages

This study shows Septin7 is essential for early hematopoiesis but dispensable at later stages, as its deletion in hematopoietic cells leads to engraftment failure but not steady-state blood lineage defects.

A Donor T-Cell Receptor Structural Signature Determines Alloreactive Potential and Predicts Acute Graft-Versus-Host Disease

The paper identifies a T-cell receptor structural signature that predicts acute GVHD risk by analyzing alloreactive clonotypes and introduces a metric to quantify clonal dominance.

Bone Mechanosensing Dictates Hematopoietic Stem Cell Fate and Immune Homeostasis

This study shows that bone mechanosensing influences HSC fate and immune homeostasis by directing lymphoid or myeloid differentiation through Piezo1-dependent pathways.

TLR7 Signaling Regulates Embryonic Hematopoietic Stem Cell Development by Sensing microRNA-146a in Vertebrates

This study identifies microRNA-146a as an endogenous activator of TLR7 signaling, which promotes embryonic HSC development through IRF5 and Notch signaling in zebrafish and mouse embryos.

USP7 maintains hematopoietic stem cell dormancy and function by stabilizing HMGA2

This study identifies USP7 as a regulator of hematopoietic stem cell dormancy by stabilizing HMGA2, showing its role in maintaining stem cell function and longevity.

The Aorta-Gonad-Mesonephros niche shapes the functions of yolk sac-derived macrophages involved in hematopoietic stem and progenitor cell generation ex vivo

This study identifies niche-specific roles of AGM macrophages in supporting HSPC generation ex vivo, highlighting F4/80's regulatory function and macrophage-derived factors.

The lack of macrophage fragment adhesion is a benchmark of dormant hematopoietic stem cells throughout the lifespan

This study identifies macrophage fragment adhesion as a marker to distinguish dormant hematopoietic stem cells from proliferative ones across the lifespan.

Hoxb5 Enriches Long-Term Hematopoietic Stem Cell Activity within the Mouse Fetal Liver Phenotypic HSC Compartment

This study identifies Hoxb5 as a marker for long-term hematopoietic stem cells in fetal liver, showing its utility in purification and revealing developmental regulation.

Hoxb5+ fetal liver hematopoietic stem cells establish lifelong hematopoiesis and exhibit enhanced ITGA4-dependent engraftment

This study identifies Hoxb5+ fetal liver HSCs as capable of lifelong hematopoiesis and shows their enhanced ITGA4-dependent engraftment compared to adult HSCs.

Niche-targeted therapy via YAP/TAZ activation enhances hematopoietic regeneration

This study shows that activating YAP/TAZ improves bone marrow niche recovery and hematopoietic regeneration after myeloablative stress.

Deploying cross-species bone marrow niches to elucidate human hematopoietic stem cell regulation in vivo

This study uses a cross-species bone marrow niche model to show that plasticity in the murine niche supports human HSC engraftment, highlighting its role in stem cell function.

Human Haematopoietic Stem Cells released into circulation following mobilization express multiple G0-associated quiescence markers

The paper investigates mobilized human HSC and finds they rapidly express G0-associated quiescence markers despite recent cell divisions.

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation

The paper identifies shared and distinct molecular mechanisms of emergency myelopoiesis using scRNA-seq and develops tools to annotate and model hematopoietic differentiation.

Spatiotemporal Proteome Remodeling Directs Human Hematopoietic Stem Cells via a Metabolic-Cytoskeletal Axis

The paper maps proteomic and spatial changes in human HSCs during ex vivo expansion, identifying a metabolic-cytoskeletal axis that coordinates structural polarity and organelle reorganization.

Architectural fragility of gene regulatory networks underlies hematopoietic stem cell aging

The paper analyzes single-cell multiome data from human HSPCs to show aging-related rewiring of gene regulatory networks, leading to stress hyperactivation, myeloid bias, and identity loss.

Activating an Interleukin-4-FLT3-STAT6 axis in Multipotent Progenitors Restores Lymphopoiesis in Inflammation and Aging

This study identifies IL-4 as a signal that restores lymphopoiesis in inflammation and aging by activating the FLT3-STAT6 pathway in multipotent progenitors.

Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Human Mobilized Peripheral Blood for Gene Therapy Applications

This study optimizes an ex vivo expansion protocol for mobilized peripheral blood HSCs, enabling robust gene therapy applications with long-term engraftment.

Single-Cell Translation and Apoptosis Profiling to Define Human CD34⁺ Cell Response to Specific Factors

The paper develops a flow cytometry protocol to measure single-cell protein synthesis and apoptosis in human CD34+ cells, revealing distinct functional profiles of small molecules in hematopoietic expansion.

A kinetics-based model of hematopoiesis reveals extrinsic regulation of skewed lineage output from stem cells

This study uses single-cell transplantation and mathematical modeling to show that lineage biases in hematopoiesis arise from extrinsic feedback and clonal competition, not intrinsic HSC properties.