Barrier Structure and Function Studies

Pericyte contact alters endothelial cell metabolism by promoting exchange of lactate through SLC16A3

This study shows that pericyte contact promotes endothelial cell metabolism by transferring lactate via SLC16A3, altering acetylome and lactylome, and enhancing EndMT-like processes.

Ischemic stroke induces persistent alteration to brain stromal progenitor cells linked to chronic vascular dysfunction

This study shows ischemic stroke causes long-term changes in brain stromal progenitor cells, leading to vascular dysfunction and potential recurrent stroke.

microRNA-184 distribution and consequences on glial septate junctions and the blood-brain barrier

This study investigates miR-184's role in Drosophila glial septate junctions and blood-brain barrier integrity, showing its absence in pleated junctions and effects on barrier function and viability.

Torsional Force by Helical Pericytes Regulates Blood Flow in Downstream Capillaries

This study shows downstream capillary pericytes express -SMA and regulate blood flow through torsional contractions, challenging the view that capillaries are passive.

Brain-derived neurotrophic factor supports pericyte and vascular homeostasis in the aging brain

This study shows BDNF supports pericyte and vascular health in aging brains by activating Akt signaling to increase PDGFRβ and CD13 expression.

CaMKKβ regulates transcription factor Elf2 gene methylation to maintain endothelial junctional barrier integrity

This study shows that CaMKKβ regulates Elf2 gene methylation, which is essential for maintaining endothelial barrier integrity by controlling Tie2 and VE-cadherin expression.

Endothelial-pericyte interactions activate insulin signaling and its implications for blood-brain barrier dysfunction in Alzheimers disease

This study investigates how pericyte degeneration contributes to blood-brain barrier disruption in Alzheimer's disease by examining insulin signaling and MMP/TIMP imbalance in an in vitro model.

Boundary homogenization and numerical modeling of solute transport across the blood-brain barrier

This study develops a novel boundary condition for modeling Aβ transport across the BBB, showing its sensitivity to receptor ratios and blood clearance rates during sleep and pathological conditions.

Pericyte and Endothelial Primary Cilia and Centrioles have Disparate Organization Across the Brain Microvasculature

The paper characterizes primary cilia and centriole organization in mouse and human brain microvasculature, revealing interspecies differences and their potential role in sensing microenvironmental signals.

PDGFRα is required for postnatal cerebral perivascular fibroblast development

This study shows that PDGFR signaling is essential for postnatal development of perivascular fibroblasts in the brain.

Elucidating Molecular Mechanisms Governing TNF-Alpha-Mediated Regulation of Amyloid Beta 42 Uptake in Blood-Brain Barrier Endothelial Cells

This study shows TNF-alpha increases A{beta}42 accumulation in BBB endothelial cells by altering endocytosis-related proteins, linking inflammation to A{beta} pathology in Alzheimer's disease.

Pervasive neurovascular dysfunction in the ventromedial prefrontal cortex of female depressed suicides with a history of childhood abuse

This study identifies widespread gene expression changes in the neurovascular unit of female depressed suicides with childhood abuse histories, highlighting key regulators like KLF2 and KLF4.

HaloTrace: A spatiotemporally precise fluorescent readout of blood-brain barrier permeability in mice

HaloTrace provides a spatiotemporal readout of BBB permeability in mice by using a fluorescent ligand that specifically accumulates in the brain at sites of BBB dysfunction.

Brendo: an open resource for uniquely transcribed genes in brain endothelial cells

The paper develops Brendo, a resource identifying BMEC-specific genes using bulk RNA-seq data and provides a web platform for exploring these genes.

Single-cell transcriptome sequencing for opening the blood-brain barrier through specific mode electroacupuncture stimulation

This study uses single-cell RNA sequencing to map cell populations in rat cerebral cortex and identifies transcriptional changes induced by electroacupuncture stimulation.

Novel VHH targeting a unique TfR1 epitope for efficient cross-species delivery of drugs in the CNS

This study identifies cross-species reactive VHHs targeting a novel TfR1 epitope, enabling efficient BBB crossing for CNS drug delivery.

Pericytes Repair Engineered Defects in the Basement Membrane to Restore Barrier Integrity in an in vitro Model of the Blood-Brain Barrier.

Inflammatory reprogramming of human brain endothelial cells compromises blood-brain barrier integrity in Alzheimer's disease

This study identifies an inflammation-driven gene module in brain endothelial cells that compromises BBB integrity in Alzheimer's disease and shows that an NF-κB inhibitor can reverse these effects.

A scalable long-term in vitro model of human colonic epithelium with continuous barrier function

This paper develops a long-term in vitro model of human colonic epithelium that maintains barrier function for up to 30 days and responds to various challenges.

Old World alphaviruses use distinct mechanisms to infect brain microvascular endothelial cells for neuroinvasion

This study identifies SLC2A3 as a key factor for Old World alphavirus neuroinvasion through BBB infection, revealing a conserved mechanism across multiple viruses.

Vascular-Perfusable Human 3D Brain-on-Chip

The paper develops a 3D Brain-Chip system using human iPSC-derived cells to model the BBB and study APOE4's role in Alzheimer's disease.

Endothelial type I interferon signaling modulates the vascular response to ischemic brain injury

This study identifies endothelial type I interferon signaling as a regulator of vascular normalization after ischemic brain injury, showing it reduces BBB disruption and promotes junctional stability.

Endothelial tight junctions and cell-matrix adhesions reciprocally control blood-brain barrier integrity

This study shows that tight junctions and cell-matrix adhesions in brain endothelial cells reciprocally control blood-brain barrier integrity through mechanical forces and protein expression changes.

Cerebral Organoids with Integrated Endothelial Networks Emulate the Neurovascular Unit and Mitigate Core Necrosis

Embedding day-8 cerebral organoids in Geltrex loaded with human brain microvascular endothelial cells, plus VEGF-supplemented mixed media, yields integrated endothelial networks and almost three-fold less apoptosis.

A multi-pore model of the blood-brain barrier tight junction strands recapitulates the permeability features of wild-type and mutant claudin-5

The paper models the blood-brain barrier tight junctions using multi-pore structures and shows that multi-Pore I replicates the permeability effect of a Cldn5 mutation.

Machine learning assisted classification of cell and brain penetrating peptides

This study classifies cell and brain penetrating peptides using machine learning, identifying key physicochemical features that distinguish brain-penetrating peptides from cell-penetrating peptides and non-BPP controls.

G3BP1 Maintains Vascular Endothelial Barrier Integrity by Negatively Regulating the MYD88-ARNO-ARF6 Signaling Pathway

This study identifies G3BP1 as a regulator of vascular endothelial barrier integrity by inhibiting the MYD88-ARNO-ARF6 pathway, showing increased permeability and reduced junction proteins in its absence.

ApoE4 disrupts intracellular trafficking and iron homeostasis in an improved iPSC-based model of human brain endothelial cells

The paper investigates how ApoE4 affects intracellular trafficking and iron homeostasis in human brain endothelial cells derived from iPSCs, showing altered endosomal organization and iron levels.

A long-lived Human Neurovascular PENTA Culture Model Reveals Incomplete Vascular Repair and Glial-Mediated Signaling After Traumatic Brain Injury.

The paper develops a human neurovascular culture model to study TBI effects, finding incomplete vascular repair and glial-mediated signaling changes.

Epigenetic Reactivation of CNS Endothelial Developmental Programs Triggers Adult Brain Angiogenesis, Promotes Post-Stroke Revascularization and Neuronal Regeneration.

This study shows that deleting Hdac2 in adult CNS endothelial cells reactivates pro-angiogenic programs, promoting revascularization and neuronal regeneration after stroke.