Pluripotent Stem Cells Research

Stage-sensitive potential of isolated rabbit ICM to differentiate into extraembryonic lineages

This study investigates the developmental potential of rabbit ICMs to differentiate into extraembryonic lineages, finding that early-stage ICMs can form a blastocyst-like structure with TE and primitive endoderm, while later-stage ICMs lose TE specification ability.

Generation of Neural Stem/Progenitor-like Cells from Cultured Human Peripheral Blood Mononuclear Cells combined with Cannabidiol

This study shows that cannabidiol can reprogram peripheral blood mononuclear cells into neural stem/progenitor-like cells through direct reprogramming.

Harnessing Dynamic Cell Responses to Optimise Pluripotency Protocols

This study designs new pluripotency maintenance protocols by leveraging cellular response dynamics to reduce drug exposure while maintaining pluripotency in mouse embryonic stem cells.

Juxtaposition of human pluripotent stem cells with amnion-like cells is sufficient to trigger primitive streak formation

This study shows that juxtaposing amnion-like cells with human pluripotent stem cells initiates gastrulation behaviors, including primitive streak formation and mesendodermal differentiation.

Derivation of cardiomyocyte-propelled motile aggregates from stem cells

The paper demonstrates a novel protocol to generate motile cardiomyocyte aggregates from mouse embryonic stem cells, enhancing their contractility and motility for biobot development.

One-step induction of human GABAergic neurons promotes presynaptic development & synapse maturation

This study develops a single-step transposon-based method to generate pure GABAergic neurons from iPSCs, showing they mature synaptically and form functional networks.

Macrophage Migration Inhibitory Factor Suppresses Natural Killer Cell Response and Promotes Hypoimmunogenic Stem Cell Engraftment Following Spinal Cord Injury

This study shows that overexpressing MIF in hypoimmunogenic iPSC-derived NPCs reduces NK cell attack and improves engraftment in SCI models.

Novel 2D/3D Hybrid Organoid System for High-Throughput Drug Screening in iPSC Cardiomyocytes

The paper develops a 2D/3D hybrid organoid system for high-throughput drug screening in iPSC cardiomyocytes, validated using doxorubicin to assess cardiotoxicity and electrophysiological effects.

Mesendodermal cells fail to contribute to heart formation following blastocyst injection

The paper shows that mesendodermal cells induced from mouse embryonic stem cells fail to contribute to heart formation when injected into blastocysts.

Analysis of astrocyte progenitors derived from human induced pluripotent stem cells in vitro and following transplantation into the intact spinal cord.

This study generates and tests astrocyte progenitor cells from hiPSCs, showing their safe survival and integration in spinal cord transplants without adverse effects.

TFAP2A+ embryonic progenitor cells undergo fate diversification to give rise to human amnion, germline, and mesoderm

The paper shows that TFAP2A+ progenitor cells generate amnion, germline, and mesoderm through BMP signaling and basement membrane cues in vitro.

Maturation of human cardiac organoids are required for disease modelling and drug discovery

This study shows that maturing human cardiac organoids enhances disease modeling and drug discovery by mimicking in vivo development and enabling therapeutic testing for DSP cardiomyopathy.

Self-Organization of Sinusoidal Vessels in Pluripotent Stem Cell-derived Human Liver Bud Organoids

This study develops a method to generate human liver bud organoids with functional sinusoidal vessels, demonstrating their potential for coagulation disorder therapies.

The generation of viable, structurally integrated human-mouse chimaeras through enhanced hPSCs proliferation

This paper enhances human pluripotent stem cell (hPSC) proliferation to improve chimaerism in human-mouse chimaeras, resulting in viable pups with integrated hPSC-derived tissues.

Donor Age Impairs Vasculogenic Potential of hiPSC-Derived Endothelial Progenitors

This study shows that donor age impairs the vasculogenic potential of hiPSC-derived endothelial progenitors, with older donor cells forming less functional vascular structures and showing epigenetic and transcriptomic differences.

Extended NGN2 Expression in iPSCs Dramatically Enhances Purity of Neuronal Cultures

The paper shows that extended NGN2 expression in iPSCs improves neuronal culture purity by reducing heterogeneity caused by suboptimal differentiation.

Healthy human induced pluripotent stem cell-derived cardiomyocytes exhibit sex dimorphism even without the addition of hormones

The paper generates hiPSC-CM from male and female donors and finds sex dimorphism in calcium handling and gene expression without hormone exposure.

Multi-omics reveal critical differentiation target for Parkinsons' Disease-vulnerable midbrain dopaminergic neurons

The paper develops a multi-omics guided differentiation protocol to generate PD-vulnerable midbrain dopaminergic neurons, showing improved yield and functional recovery in mice.

Non-viral derivation of induced pluripotent stem cells from the canine umbilical cord

This study generates non-viral canine induced pluripotent stem cells (iPSCs) from umbilical cord fibroblasts, confirming their pluripotency and differentiation potential.

FOXM1 Inhibition Promotes Polyploidization and Metabolic Maturation in Human iPSC-Derived Hepatocytes by Modulating the Wnt/β-Catenin Pathway

This study shows that FOXM1 inhibition promotes terminal differentiation of human iPSC-derived hepatocytes by enhancing polyploidization and metabolic maturation through the Wnt/β-catenin pathway.

Scalable expansion and hepatic zone maturation of hepatic progenitor cells from human pluripotent stem cells

This study develops a method to generate functional hepatocytes from human pluripotent stem cells through controlled differentiation and tissue engineering.

An iPSC-based iMSC Model for Osteogenesis Imperfecta: A Platform for Disease Modeling and Drug Screening

This paper develops an iPSC-derived iMSC model of Osteogenesis Imperfecta that recapitulates disease phenotypes and enables drug screening.

Pioneer factor ETV2 safeguards endothelial cell specification by recruiting the repressor REST to restrict alternative lineage commitment

This study identifies ETV2's role in endothelial cell specification by recruiting REST to repress alternative lineage genes through single-cell RNA-seq and ATAC-seq analyses.

Injectable 3D microcultures enable intracerebral transplantation of mature neurons directly reprogrammed from patient fibroblasts

This study develops a 3D microculture method to generate mature dopamine neurons from patient fibroblasts, which can be transplanted into a Parkinson's disease rat model to form functional neuron grafts.

A single factor for safer cellular rejuvenation

This paper identifies SB000, a single gene factor that rejuvenates cells from multiple germ layers without inducing pluripotency, rivaling the effectiveness of Yamanaka factors.

Micropatterned Organoids Enable Modeling of the Earliest Stages of Human Cardiac Vascularization

The paper demonstrates that micropatterned human pluripotent stem cells can model early human cardiac vascularization in vitro, resembling a 6.5 post-conception week heart and revealing conserved vascular development programs.

Engineering a Controlled Cardiac Multilineage Co-Differentiation Process Using Statistical Design of Experiments

This study engineers a controlled cardiac multilineage co-differentiation process using statistical design of experiments to generate cardiomyocytes, mural cells, and endothelial cells from iPSCs with high controllability and maturity.

A new resource of clonal pluripotent human stem cell lines exhibiting inter- and intra-embryo consistency and variability

The paper describes a resource of clonal human pluripotent stem cell lines derived from embryos, showing intra- and inter-embryo variation and potential for modeling chromosomal mosaicism.

Discovery of NANOG enhancers and their essential roles in self-renewal and differentiation in human embryonic stem cells

The paper identifies two NANOG enhancers in human embryonic stem cells and shows their critical role in maintaining self-renewal and preventing differentiation.

Reconstitution of adrenocortical functional zonation from human pluripotent stem cells

This paper develops a human pluripotent stem cell-derived adrenal organoid system that recapitulates adrenal zonation and demonstrates its utility for studying adrenal development and disease.