Pluripotent Stem Cells Research

Lineage recording in monoclonal gastruloids reveals heritable modes of early development

The paper develops monoclonal gastruloids from single mouse embryonic stem cells and uses DNA Typewriter to reveal heritable lineage biases in early development.

Barcoded monoclonal embryoids are a potential solution to confounding bottlenecks in mosaic organoid screens

The paper develops monoclonal embryoids with genetic barcoding to overcome clonal complexity bottlenecks in mosaic organoid screens, enabling more accurate quantification of genetic perturbation effects.

3D organoids containing endothelial and neural cells generation by serial inductions of differentiation on human iPSC-derived embryoid bodies

This paper develops 3D brain organoids with endothelial and neural cells using sequential differentiation of iPSC-derived embryoid bodies, showing enhanced angiogenesis and SARS-CoV-2 receptor expression.

Tissue resident macrophages innately develop in a human iPSC-derived cardiac organoid model

This study develops a human iPSC-derived cardiac organoid model that includes macrophages and mimics aspects of cardiogenesis.

Machine-guided cell-fate engineering

The paper introduces a machine-learning pipeline, CellCartographer, to design TF pooled-screens for cell type conversion using chromatin accessibility data, achieving efficient differentiation of iPSCs into six cell types within 6 days.

Selective GSK3α Inhibition Promotes Self-Renewal Across Different Stem Cell States

This paper shows that selective GSK3 inhibition with BRD0705 supports self-renewal of mouse stem cells and enables co-culture of naive and primed stem cells while preserving their identities.

Automated high-throughput patch clamp electrophysiology of hiPSC-derived neuronal models

This paper introduces automated high-throughput patch clamp electrophysiology methods for hiPSC-derived neurons, enabling efficient analysis of voltage-gated sodium channels and pharmacological responses.

An efficient protocol to derive primordial germ cell like cells in vitro from murine embryonic stem cells.

This paper develops an optimized protocol to improve the efficiency of in vitro differentiation of primordial germ cell-like cells from mouse embryonic stem cells using ascorbic acid.

Extended culture of 2D gastruloids to model human mesoderm development

The paper extends the culture of 2D gastruloids to 96h, revealing a phase of mesoderm differentiation with spatial organization similar to embryos.

Modeling tissue-resident macrophage development from mouse pluripotent stem cells

This paper develops a protocol to efficiently generate tissue-resident macrophages from mouse pluripotent stem cells, enabling in vitro and in vivo modeling of their development.

TCA cycle rewiring underpins implantation and histone acetylation programming

This study reveals that TCA cycle rewiring at implantation supports histone acetylation programming and pluripotency transitions in mouse embryos.

Generation of iPSC-derived CD4+ Th1 cells enhancing chimeric antigen receptor-T cell cytotoxicity

This study generates CD4+ Th1 cells from iPSCs to enhance CD8+ CAR-T cell cytotoxicity against GD2-expressing lymphoma.

Hypoxia and loss of GCM1 expression prevents differentiation and contact inhibition in human trophoblast stem cells

Human Plasma-Like Medium Promotes Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes

This paper shows that Human Plasma-Like Medium (HPLM) improves the maturation of human pluripotent stem cell-derived cardiomyocytes compared to conventional media.

MIXL1 Activation in Endoderm Differentiation ofHuman Induced Pluripotent Stem Cells

The paper shows that activating MIXL1 in hiPSCs improves endoderm differentiation and maturation.

Characterization of Human Anterior Neural Organoids as a Model for Investigating Cohen Syndrome

This study develops a method to generate human anterior neural organoids (hANOs) and uses them to model microcephaly in Cohen syndrome by analyzing gene expression and cellular phenotypes.

Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells

This study shows Matrigel inhibits elongation and promotes endoderm differentiation in mouse embryonic stem cell aggregates, suggesting its effects are primarily biochemical rather than physical.

Self-Assembled Generation of Multi-zonal Liver Organoids from Human Pluripotent Stem Cells

This study develops self-assembled multi-zonal liver organoids from human pluripotent stem cells, revealing zonal-specific functions and epigenetic mechanisms that mimic human liver architecture.

Integrated Molecular-Phenotypic Profiling Reveals Metabolic Control of Morphological Variation in Stembryos

The paper identifies early metabolic imbalances as drivers of phenotypic variation in stembryos and shows how metabolic interventions can correct aberrant morphology and lineage bias.

Human induced pluripotent stem cell-derived astrocyte and neuron co-culture model for neuroinflammation modeling

This study differentiates hiPSCs into midbrain dopaminergic neurons and astrocytes, showing that co-culture is necessary for maturation and suitable for modeling astrocyte-driven neuroinflammation.

Identification of novel candidate regulators of human naive pluripotency by means of a genetic switch utilizing the chimeric receptor G-CSFR:GP130

This study identifies novel regulators of human naive pluripotency using a genetic switch that activates STAT3 and JAK signaling, revealing key genes and mechanisms involved in reprogramming.

Hypoimmunogenic hPSC-derived cardiac organoids for immune evasion and heart repair

This study develops hypoimmunogenic hPSC-derived cardiac organoids that evade immune rejection and repair damaged hearts in animal models.

The regulatory architecture of the primed pluripotent cell state

The paper identifies 132 master regulators of primed pluripotency in mouse epiblast stem cells and constructs a regulatory network revealing four functionally distinct modules.

Termination sequence between an inducible promoter and ubiquitous chromatin opening element (UCOE) reduces gene expression leakage and silencing

The paper investigates how spacer sequences between a promoter and A2UCOE affect gene expression leakage and silencing in iPSCs, finding that an AT-rich poly-A terminator reduces leakage and enhances anti-silencing.

Cryopreservation of Human Cortical Organoids Using Vitrification.

This paper demonstrates that vitrification can effectively cryopreserve human cortical organoids, maintaining their structure, cell type composition, and function.

Differentiation Protocol-Dependent Variability in hiPSC-Derived Endothelial Progenitor Functionality

This paper compares two hiPSC differentiation protocols for endothelial progenitors, finding significant differences in cell maturity, phenotype, and vasculogenic potential.

Orchestration of pluripotent stem cell genome reactivation during mitotic exit

This study investigates how pluripotent stem cells reactivate gene expression and rebind transcription factors during mitotic exit using a novel temporal separation method.

Axial patterning of gastruloids via diverse cell type compositions

This study investigates how varying proportions of primed and naive ESCs affect gastruloid formation and AP axial patterning, revealing developmental flexibility in mammalian embryogenesis.

Reconstitution of human fetal ovaries reveals niche requirements for primordial germ cell-like cell progression

This study shows that human primordial germ cell-like cells can mature in reconstituted human fetal ovaries and amnion-like cell aggregates, with SCF being crucial for hPGCLC survival.

DGCR8 haploinsufficiency leads to primate-specific RNA dysregulation and pluripotency defects

This study shows that DGCR8 haploinsufficiency in human pluripotent cells causes RNA dysregulation and pluripotency defects, with rescue by primate-specific miRNAs.