Heat shock proteins research

Domain-specific folding of the tandem β-propeller protein Coronin 7 (Coro7) by CCT/TRiC

This study identifies Coronin 7 as a CCT/TRiC substrate and shows that CCT/TRiC interacts specifically with the first β-propeller domain of Coro7.

HSP-1-Specific Nanobodies Alter Chaperone Function in vitro and in vivo

This study develops two nanobodies that specifically target HSP-1, reducing its ATPase activity and protein folding capacity both in vitro and in vivo.

BAG6 and RNF126 are broadly involved in protein quality control of non-native missense protein variants

The paper identifies BAG6 and RNF126 as key players in PQC degradation of non-native missense protein variants using CRISPR screens and VAMP-seq.

Characterization of heat shock protein expression and its application to temporal gene expression in the nematode Pristionchus pacificus

The paper identifies a heat shock promoter in Pristionchus pacificus for temporal gene expression by analyzing heat-induced gene expression through RNA-seq and reporter transgenesis.

Transcriptional responses to proteotoxic stressors are profoundly diverse and tissue-specific

The paper examines transcriptional responses to different proteotoxic stressors and identifies tissue-specific adaptations in Huntington's Disease mice.

Cryo-EM structures of human ClpXP reveal mechanisms of assembly and proteolytic activation

This study presents cryo-EM structures of human ClpXP, revealing mechanisms of assembly and proteolytic activation through conformational changes and interactions.

The Heat shock protein 70 machinery is crucial in the production of infectious chikungunya virus progeny

This study shows that inhibiting Hsp70 reduces chikungunya virus infectious particle production without harming host cells.

Disrupted TRiC allostery and conformational equilibrium by LCA-linked mutations: structural and functional insights

This study investigates how LCA-linked mutations in TRiC/CCT subunit CCT2 disrupt its allosteric network and functional cycle, leading to structural and functional defects associated with Leber Congenital Amaurosis.

Co-translational determination of quaternary structures in chaperone factories

The paper identifies co-translational interactions between R2TP and client proteins, revealing a mechanism called co-translational chaperone channeling that facilitates early assembly of protein complexes.

Stress testing reveals selective vulnerabilities in protein homeostasis

The paper uses transposon sequencing to identify stress-specific vulnerabilities in protein homeostasis by analyzing gene fitness under proteotoxic stresses in bacteria.

A Stochastic Mechanism Drives Fast Substrate Translocation in the AAA+ Machine ClpB

The paper uses single-molecule FRET to show ClpB translocates substrates rapidly and stochastically, challenging previous models of ATP-driven mechanical work.

On adaptation to a mesophilic environment and the chaperone network in Archaea

The paper analyzes archaeal proteomes to show a correlation between HSP70 family presence and optimal growth temperature, suggesting HSP70s enabled adaptation to mesophilic environments and eukaryotic emergence.

Recognition of small Tim chaperones by the mitochondrial Yme1 protease

This study investigates how Yme1 protease selects Tim10 as a substrate, showing preferential binding and degradation after disulfide bond disruption.

The nucleotide exchange factor, GrpE, modulates substrate affinity by interaction of its N-terminal tails with the DnaK substrate-binding domain.

This paper investigates how GrpE modulates DnaK's substrate affinity through interactions between GrpE's N-terminal tails and DnaK's SBD, revealing a temperature-dependent mechanism.

Transcription dynamics and regulation of heat shock protein genes during stress and development in the estuarine cnidarian Nematostella vectensis

This study characterizes the diversity and expression patterns of HSP70 and HSP90 genes in Nematostella vectensis across developmental stages and temperature changes, revealing distinct regulatory mechanisms.

Origin of Class B J-domain proteins involved in amyloid transactions

This paper investigates the evolutionary origin of class B J-domain proteins, showing they evolved from class A duplicates by losing their zinc finger domain, enabling new functions in amyloid aggregate regulation.

ClpA- and ClpAP-Catalyzed Unfolding and Translocation are Differentially Coupled to ATP Binding

This paper measures the ATP-dependent unfolding and translocation rates of folded substrates by ClpA and ClpAP, showing differential coupling to ATP binding and allosteric regulation by ClpP.

Genome-wide identification of heat shock proteins (HSP70) in the Cuban painted snail Polymita picta: evolutionary constraints face climate change challenges

The paper identifies and characterizes HSP70 family members in the Cuban painted snail Polymita picta to assess their role in thermoresistance and adaptation to climate change.

Dominant-negative TP53 mutations potentiated by the HSF1-regulatedproteostasis network

The paper shows that HSF1 activation supports the emergence of cancer-associated p53 mutations by enhancing their fitness under nutlin-3 pressure.

Small-molecule inhibitor of C-terminal HSP90 dimerization modulates autophagy and functions synergistically with mTOR inhibition to kill cisplatin-resistant cancer cells

This paper introduces a C-terminal HSP90 dimerization inhibitor, VWK147, which induces cell death in cisplatin-resistant cancer cells and synergizes with mTOR inhibition to enhance therapeutic efficacy.

The essential co-chaperone Sgt1 regulates client dwell time in the Hsp90 chaperone cycle

This study identifies Sgt1 as an essential co-chaperone that stabilizes Hsp90-client complexes and prevents dissociation by Aha1, enhancing client maturation efficiency.

Structural studies of mycobacterial HptG reveal a silent state and offer insights into TLR4 activation

This study presents the first crystal structure of HtpGMtb, revealing a conformationally silent dimer and direct TLR4 interaction, providing insights into its catalytic activity and TLR4 activation mechanism.

Small heat shock protein HSPB5 uses disorder to bind zinc with high affinity.

This study shows HSPB5 binds zinc with high affinity using its disordered N-terminal region, acting as a zinc reservoir under oxidative stress.

Targeting the Protein-Protein Interaction Between the CDC37 Co-Chaperone and Client Kinases by an Allosteric RAF Dimer Breaker

This study investigates Braftide's dual effect on RAF dimer disruption and CDC37-client kinase interaction, showing proteasomal degradation and therapeutic potential.

ClpK as a Chaperone Hub: Computational Exploration of its Protein Interaction Landscape.

This study uses computational methods to predict and analyze ClpK protein interactions in Klebsiella pneumoniae, identifying KPN_01323 as a promising interaction partner.

Heat Shock-Induced PI(4)P Increase Drives HSPA1A Translocation to the Plasma Membrane in Cancer and Stressed Cells through PI4KIII Alpha Activation

Structural insights into autophosphorylation-mediated heat shock response by the protein-arginine kinase McsAB

This study reveals the structural mechanism of McsAB-mediated suppression of CtsR during heat shock, showing autophosphorylation is essential for rapid response.

Functional Characterization of Hsp110 in Drosophila Reveals its Essential and Dosage-Sensitive Role in Nervous System Integrity

This study investigates the role of Hsp110 in Drosophila, showing its essential and dosage-sensitive function in nervous system development and survival.

Mutations in Hsp40 co-chaperone change the unique canonical inter-domain interactions stimulating LGMDD1 myopathy

The paper investigates how mutations in the J-domain of DNAJB6 affect chaperone function and inter-domain interactions, providing mechanistic insights into LGMDD1 myopathy.

Unraveling proteomic chaos by independent component analysis - ClpX proficiency promotes the iron and oxygen limitation responses of Staphylococcus aureus and affects the intracellular bacterial behavior

This study uses proteomics and independent component analysis to show ClpX regulates S. aureus responses to iron and oxygen limitation and affects bacterial fitness and virulence.