Nuclear Structure and Function

A longevity-associated ubiquitin E3 ligase UBE3C governs lamin B1 homeostasis through selective autophagy and delay senescence

This study identifies UBE3C as a longevity-associated E3 ligase that maintains nuclear lamina integrity by modulating LMNB1 and LBR through selective autophagy, delaying senescence.

Nucleolar endonuclease MBLAC2 orchestrates co-transcriptional ribosomal RNA processing and global transcriptional output

This study identifies MBLAC2 as a nucleolar nuclease essential for co-transcriptional rRNA processing and global transcriptional output.

Sequence space of Xpo1-dependent NESs reveals a functional affinity ceiling

The paper explores the sequence space of Xpo1-dependent NESs using high-throughput screening and identifies key residues critical for NES activity, revealing an affinity ceiling that explains natural NES limitations.

Asynchronous structural rearrangements govern constriction of the human nuclear pore complex

The paper uses a structure-based model to study the asynchronous structural rearrangements during nuclear pore complex constriction and their energetic contributions.

Amphipathic helices sense the inner nuclear membrane environment through lipid packing defects

The paper identifies how amphipathic helices bind to the inner nuclear membrane by sensing lipid packing defects rather than electrostatics, showing the INM's loose packing under basal conditions.

A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments

The paper introduces a method to pulse-label yeast Nuclear Pore Complexes using a nanobody, enabling tracking in imaging and biochemical experiments.

The Anaphase Promoting Complex targets the toxic protein Progerin for ubiquitin-dependent degradation via autophagy

This study identifies the Anaphase Promoting Complex (APC) as a mediator of progerin degradation via autophagy in Hutchinson-Gilford Progeria Syndrome.

The chaperone Jjj2 regulates nucleoporin condensation in budding yeast

This study identifies Jjj2 as a chaperone that regulates nucleoporin condensation in budding yeast, showing its role in maintaining NPC permeability and preventing aggregation.

Nuclear size and physical properties of the nucleoplasm are determined by colloid osmotic pressure at the nuclear envelope

Overexpressing mCherry-GST targeted to the nucleus or cytoplasm in fission yeast shifts the nuclear-to-cell volume ratio as a parameter-free colloid osmotic model predicts, retuning nucleoplasm diffusivity, condensates and heterochromatin.

Nuclear envelope-dependent heterochromatin positioning gates the timing of nucleolar assembly in the early embryo

The paper shows that the LINC complex and nuclear lamina delay nucleolar assembly in C. elegans embryos by organizing heterochromatin, with CEC-4 playing a key role in suppressing premature nucleoli.

Mechanically induced pre-mitotic nuclear deformations promote nuclear rupture through lamin B1 depletion

The paper shows that microtubule-dependent nuclear invaginations cause lamin B1 depletion and nuclear rupture through mechanical stress, aiding mitotic entry.

Granular component sub-phases direct ribosome biogenesis in the nucleolus

The paper uses super-resolution microscopy to show that nucleolus granular component sub-phases are organized into multiphase condensates that facilitate ribosome subunit assembly.

Transcription controls chromatin-nuclear lamina contacts through distinct Lamin A and LBR tethering mechanisms

This study shows that transcription inhibition rapidly and reversibly rewires chromatin-nuclear lamina contacts through distinct Lamin A and LBR tethering mechanisms.

VPS4 and CHMP7 release centromeres from the nuclear envelope for post-mitotic positioning in daughter nuclei

This study shows that VPS4 and CHMP7 are required for releasing centromeres from the nuclear envelope after mitosis, preventing DNA damage and ensuring proper positioning.

A conserved mechanism of membrane fusion in nuclear pore complex assembly

This study identifies Brl1 and Brr6 as conserved fusogens mediating membrane fusion during NPC assembly in eukaryotes.

Lamins gate nuclear and chromatin structures for cardiomyocyte maturation genes

This study shows lamin-B1 is essential for cardiomyocyte maturation and survival by maintaining chromatin organization and transcription programs during heart development.

Mechanoresponsive modulation of nuclear pore complex structure and function by O-GlcNAc

This study shows that O-GlcNAc modification of nucleoporins modulates nuclear pore complex permeability in response to mechanical and osmotic cues.

The nucleolus is a mechanosensitive condensate that adapts ribosome biogenesis to mechanical forces

This study shows mechanical compression remodels the nucleolus, reduces rDNA transcription, and identifies nucleolin as a mediator of this response through biophysical and epigenetic mechanisms.

A pre-rRNA positive feedback loop drives malignant ribosome biogenesis

This study identifies a pre-rRNA positive feedback loop driven by CK2 in the nucleolus that promotes malignant ribosome biogenesis and cancer growth.

CRISPR-Engineered hiPSC-derived Cardiomyocytes Reveal Divergent Responses to Loss and Defective Processing of A-type Lamins

This study compares the effects of lamin A/C loss and defective prelamin A processing in cardiomyocytes using CRISPR-engineered hiPSC-derived cells, revealing shared and distinct stress responses.

Ionic Exposure History Shapes Inner Nuclear Membrane Voltage and Chromatin Texture Responses

This study shows that ionic exposure history affects inner nuclear membrane voltage and chromatin texture in NRK cells, with responses dependent on exposure order and chromatin state.

Mlp1 and Mlp2 cooperate to build a stoichiometric nuclear pore basket in budding yeast

The paper investigates the interactions required for nuclear basket formation in budding yeast and proposes a refined model with a 4:2:1 stoichiometry of Mlp1:Mlp2:Pml39 per spoke.

Coordinated regulation of Barrier-to-Autointegration Factor (BAF) phosphorylation by protein phosphatases PP2A and PP4

This study identifies PP2A as the main phosphatase for BAF dephosphorylation in interphase and shows PP4's role in maintaining centromeric BAF in an unphosphorylated state during mitosis.

Coupled nucleoplasmic flows and envelope expansion govern the mechanical resistance of the nucleus

This study proposes a physical model of the nucleus that explains its mechanical behavior under deformation, highlighting the role of the envelope and chromatin in nuclear mechanics.

Msc1 accumulates at and excludes nuclear pore complexes from nucleus-vacuole junctions.

This study shows Msc1 in yeast localizes to nucleus-vacuole junctions and excludes NPCs, maintaining NPC homeostasis during quiescence preparation.

Progerin-Induced Nuclear Envelope Remodeling is Shaped by Cell Division and NUP153

This study shows progerin causes nuclear envelope remodeling in two stages, with NUP153 promoting invagination, and links these changes to aging-related nuclear morphology.

Prostaglandins regulate the nucleoskeleton during Drosophila border cell migration

This study shows that prostaglandin signaling regulates nucleoskeletal remodeling during Drosophila border cell migration, which facilitates collective migration.

Lamin A/C coordinates nuclear mechanics, chromatin architecture, and transcriptional homeostasis in skeletal muscle in vivo

This study investigates how Lamin A/C disruption affects nuclear mechanics, chromatin architecture, and transcription in skeletal muscle in vivo, finding that it alters chromatin organization and gene expression in mechanically stiff tissues.

Polarization Increases Nuclear Stiffness in Macrophages Despite Reduction in Lamin A/C Levels

This study shows that pro-inflammatory polarization of macrophages increases nuclear stiffness despite reduced lamin A/C levels, suggesting chromatin changes drive nuclear mechanics.

Lamin B1 Safeguards the B Cell Genome and Shapes Lymphoma Outcome

This study shows that Lamin B1 protects B cell genomes by preventing DNA damage and regulating chromatin accessibility, with its loss linked to poor lymphoma outcomes.