Pluripotent Stem Cells Research

Direct induction of human neurons from fibroblasts carrying the neuropsychiatric 22q11.2 microdeletion reveals transcriptome- and epigenome-wide alterations

This paper develops a method to generate neuronal cells from adult fibroblasts and uses it to study gene expression and epigenetic changes in 22q11.2 deletion syndrome.

Modular Derivation and Unbiased Single-cell Analysis of Regional Human Hindbrain And Spinal Neurons Enables Discovery of Nuanced Transcriptomic Patterns along Developmental Axes

The paper develops a modular differentiation system to generate diverse human hindbrain and spinal neurons and uses computational methods to identify region-specific transcriptomic patterns.

Loss of Resf1 reduces the efficiency of embryonic stem cell self-renewal and germline entry

This study shows that RESF1 loss reduces ESC self-renewal and PGCLC differentiation efficiency in mice, but not NANOG-driven self-renewal without LIF.

Enhancer-associated H3K4 methylation safeguards in vitro germlinecompetence

The paper shows that H3K4me1 retention in enhancers supports germline competence by maintaining enhancer accessibility and responsiveness during in vitro differentiation.

Overcoming the effect of ASH1L haploinsufficiency on stem cells amenability to genome editing and differentiation into the neuronal lineage - a technical report

This paper describes protocols to overcome ASH1L haploinsufficiency in stem cells, improving genome editing and neuronal differentiation efficiency.

iPSC culture expansion selects against putatively actionable mutations in the mitochondrial genome

The paper analyzes mtDNA variants in iPSCs and finds that culture expansion selects against harmful mitochondrial mutations, while reprogramming and differentiation do not significantly alter mtDNA heteroplasmy.

Reconstructing human early embryogenesis in vitro with pluripotent stem cells

This study uses human pluripotent stem cells to create an in vitro model of early human embryogenesis, recapitulating key developmental events from embryonic day 3 to day 9/10.

Transcriptomic entropy benchmarks stem cell-derived cardiomyocyte maturation against endogenous tissue at single cell level

The paper develops a transcriptomic entropy metric to benchmark maturation of PSC-derived cardiomyocytes against endogenous tissue using scRNA-seq data.

Generation of human blastocyst-like structures from pluripotent stem cells

This paper describes a method to generate blastocyst-like structures from human pluripotent stem cells and shows they resemble blastocysts in morphology and transcriptome.

Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease Risk

This study develops a method to efficiently generate dopaminergic neurons from hiPSCs and shows they model psychiatric disease risk genes.

The second lineage differentiation of bovine embryos fails in the absence of OCT4/POU5F1

This study shows OCT4 is essential for maintaining pluripotency in the epiblast and differentiation of the hypoblast in bovine embryos.

Generation of cardiac valve endocardial like cells from human pluripotent stem cells

This paper describes a method to generate valve endocardial progenitor cells from human pluripotent stem cells and shows they resemble primary valve endothelial cells.

NOCICEPTRA: Gene and microRNA signatures and their trajectories characterizing human iPSC-derived nociceptor maturation

The paper characterizes human iPSC-derived nociceptor maturation using gene and microRNA expression trajectories and introduces a platform for querying these data.

The Aryl Hydrocarbon Receptor Promotes Differentiation During Mouse Preimplantational Embryo Development

This study shows that AhR promotes differentiation during mouse preimplantational embryo development by regulating pluripotency factors and metabolic activity.

Bioengineered embryoids mimic post-implantation development in vitro

This study develops EpiTS embryoids, which mimic post-implantation development in vitro by combining ESC and TSC aggregates to model gastrulation and axial morphogenesis.

BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo

The paper compares BMP-treated human embryonic stem cells to amnion cells in monkey embryos, finding they are more transcriptionally similar to amnion cells than trophectoderm cells.

Extracellular cues accelerate neurogenesis of induced pluripotent stem cell derived neurons

This study accelerates and standardizes the maturation of iPSC-derived neurons by optimizing extracellular cues, resulting in synchronized neurogenesis and earlier calretinin expression.

Human brain organoids assemble functionally integrated bilateral optic vesicles

This study shows human brain organoids can form bilateral optic vesicles with cellular diversity and functionality, including light-responsive photoreceptors and neuronal networks.

Efficient and Rapid generation of neural stem cells by direct conversion Fibroblasts with A Single microRNA

This study generates neural stem cells from fibroblasts using a single microRNA, achieving high purity and functionality in 2-3 days.

Responsiveness to perturbations is a hallmark of transcription factors that maintain cell identity

The paper identifies transcription factors that maintain cell identity by analyzing their responsiveness to perturbations in human iPSC-derived cardiac myocytes and fibroblasts.

Somatic Cell Nuclear Transfer Embryos Show Massive Dysregulation of Genes Involved in Transcription Pathway

The paper identifies transcription pathway dysregulation as a barrier to nuclear transfer embryo development using RNA-seq data analysis.

Microfabricated disk technology: rapid scale up in midbrain organoid generation

This study develops microfabricated disks to scale up midbrain organoid generation, reducing labor and variability while maintaining viability for Parkinson's disease research.

Identification and characterization of hPSC-derived FOXA2+ progenitor cells with ventricular cardiac differentiation potential

This study identifies and characterizes hPSC-derived FOXA2+ progenitor cells with enhanced ventricular cardiomyocyte differentiation potential.

Lineages of embryonic stem cells show non-Markovian state transitions

This study shows embryonic stem cells do not transition between states in a memoryless manner, indicating non-Markovian behavior in their developmental processes.

Modeling lung cell development using human pluripotent stem cells

This study uses human pluripotent stem cells to model lung development, generating renewable lung epithelial spheroids and identifying new cell lineage relationships.

TGF-β modulates cell fate in human ES cell-derived foregut endoderm by inhibiting multiple endogenous signaling pathways

This study identifies how TGF-β1 modulates cell fate in human ES cell-derived foregut endoderm by inhibiting multiple signaling pathways, promoting pancreas fate over liver.

AF10 (MLLT10) prevents somatic cell reprogramming through regulation of H3K79 methylation

This study identifies AF10 as a barrier to somatic cell reprogramming by regulating H3K79 methylation, showing that its suppression enhances reprogramming efficiency.

Derivation of human extended pluripotent stem cells in feeder-free condition

This study establishes a feeder-free method to generate human extended pluripotent stem cells (EPSCs) using Matrigel and chemical compounds, demonstrating their pluripotency through molecular and chimeric assays.

Inhibition of N-myristoyltransferase Promotes Naive Pluripotency in Mouse and Human Pluripotent Stem Cells

This study shows that inhibiting N-myristoyltransferase (Nmt) promotes naive pluripotency in both mouse and human pluripotent stem cells.

Single-cell sequencing of human iPSC-derived cerebellar organoids shows recapitulation of cerebellar development

The paper develops a method to generate cerebellar organoids from human iPSCs and shows they recapitulate key aspects of cerebellar development through single-cell profiling.