Pluripotent Stem Cells Research

The combined action of Esrrb and Nr5a2 is essential for naive pluripotency.

This study shows that Esrrb and Nr5a2 are essential for maintaining naive pluripotency in mouse ESCs by regulating the pluripotency network and preventing differentiation.

Differential regulation of lineage commitment in human and mouse primed pluripotent stem cells by NuRD

This study compares NuRD's role in human and mouse pluripotent stem cells, finding that NuRD is required for transcriptional responses to differentiation signals in both, but with distinct functional differences between species.

Chromatin remodeler Brahma safeguards canalization in cardiac mesoderm differentiation

This study shows that Brm deletion causes identity switches in cardiac mesoderm differentiation by altering chromatin accessibility and gene expression, revealing BRM's role in safeguarding developmental canalization.

Human iPSC-derived alveolar and airway epithelial cells can be cultured at air-liquid interface and express SARS-CoV-2 host factors

Human iPSC-derived alveolar type II-like and airway epithelial cells adapted to two-dimensional air-liquid interface culture express ACE2 and TMPRSS2 at frequencies comparable to primary lung epithelial controls.

Direct reprogramming of adult hepatocytes to generate LGR5+ endodermal progenitor

This paper describes a method to convert adult hepatocytes into LGR5+ endodermal progenitor cells using transcription factors and activators, enabling liver tissue regeneration and disease modeling.

Heat shock induces the depletion of Oct4 in mouse blastocysts and stem cells

This study shows heat shock reduces Oct4 levels in mouse blastocysts and stem cells via the ubiquitin-proteasome pathway involving Dapk1 and Pin1.

FUNCTION OF PUMILIO GENES IN HUMAN EMBRYONIC STEM CELLS AND THEIR EFFECT IN STEMNESS AND CARDIOMYOGENESIS

This study investigates the role of PUM1 and PUM2 in human ESCs, showing their involvement in maintaining pluripotency and enhancing cardiomyogenic differentiation.

Mutational impact of culturing human pluripotent and adult stem cells

This study quantifies and characterizes mutation accumulation in human induced pluripotent stem cells and adult stem cells during in vitro culture, revealing distinct mutational signatures and higher mutation rates compared to in vivo.

Characterization of human-iPSCs derived spinal motor neurons by single-cell RNA sequencing

The paper uses single-cell RNA sequencing to characterize human iPSC-derived spinal motor neuron subtypes and identifies their molecular profiles.

Characterization of human iPSC-derived astrocytes with potential for disease modeling and drug discovery

This study derives and characterizes human iPSC-derived astrocytes, showing they exhibit physiological traits and respond to inflammatory stimuli, making them suitable for disease modeling and drug discovery.

Developmental co-emergence of cardiac and gut tissues modeled by human iPSC-derived organoids

This study shows that human iPSC-derived organoids can model the cooperative development of cardiac and gut tissues through paracrine signaling.

Single Cell Analysis Reveals Partial Reactivation of X-chromosome Instead of chromosome-wide dampening in Naïve Human Pluripotent Stem Cells

The paper uses single-cell analysis to show partial reactivation of the inactive X-chromosome in naive human pluripotent stem cells, challenging the notion of chromosome-wide dampening.

NCOR1 AND OCT4 FACILITATE EARLY REPROGRAMMING BY CO-SUPPRESSING FIBROBLAST GENE EXPRESSION

This study shows NCOR1 and OCT4 co-suppress fibroblast gene expression during early reprogramming, enabling successful iPSC generation by overcoming reprogramming bottlenecks.

Robust Production of Uniform Human Cerebral Organoids from Pluripotent Stem Cells

This study develops a robust method to produce uniform human cerebral organoids with consistent morphology and cell type composition, achieving an 80% success rate.

Doxycycline Significantly Enhances Induction of iPSCs to Endoderm by Enhancing survival via AKT Phosphorylation

This study improves iPSC differentiation into endoderm by using doxycycline to enhance survival via AKT phosphorylation, achieving 90% endoderm formation in over 70 iPSC lines.

Establishment of a Human Induced Pluripotent Stem Cell-Derived Neuromuscular Co-Culture Under Optogenetic Control

This paper establishes a hiPSC-derived neuromuscular co-culture system under optogenetic control to study NMJ formation and disease mechanisms.

Therapeutic Reversal of Chemically Induced Parkinson Disease by Converting Astrocytes into Nigral Neurons

This study converts astrocytes into functional neurons to reverse Parkinson's disease in mice by suppressing PTB, restoring dopamine levels and nigrostriatal pathways.

Cooperative molecular networks drive a mammalian cell state transition

The paper identifies molecular networks driving the transition from naive to formative pluripotency in mammalian cells using genetic and transcriptomic analyses.

Dppa2/4 promotes zygotic genome activation by binding to GC-rich region of proximal promoter in signaling pathways

The paper identifies Dppa2/4 as key regulators of zygotic genome activation by binding GC-rich regions of promoters and activating critical signaling pathways in mouse and human embryos.

High-throughput propagation of human prostate tissue from induced-pluripotent stem cells

This paper introduces a high-throughput method to generate human prostate tissue from induced pluripotent stem cells using co-culture with urogenital sinus mesenchyme.

Changing the Waddington landscape to control mesendoderm competence

This study shows that mesendoderm competence in differentiating human pluripotent stem cells can be modulated by genetic perturbations and tracked using a low-dimensional reaction coordinate.

Scalable generation of mesenchymal stem cells and adipocytes from human pluripotent stem cells

This paper develops a scalable method using all-trans-retinoic acid to generate mesenchymal stem cells and adipocytes from human pluripotent stem cells with enhanced differentiation potential.

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

This study shows PRDM14 is essential for human PGC fate, contradicting previous findings, and reveals divergent regulatory mechanisms between human and mouse PGCs using inducible degrons.

Identification of X-chromosomal genes that drive global X-dosage effects in embryonic stem cells

The paper identifies X-chromosomal genes, including Klhl13, that drive sex differences in murine embryonic stem cells by affecting pluripotency and differentiation.

Vascular Progenitors Generated from Tankyrase Inhibitor-Regulated Naive Diabetic Induced Pluripotent Stem Cells Potentiate Efficient Revascularization of Ischemic Retina

This paper shows that reprogramming diabetic fibroblasts into naive hiPSC using tankyrase inhibitors improves vascular progenitor generation and revascularization in ischemic retinas.

RNA-Seq analysis reveals pluripotency-associated genes and their interaction networks in human embryonic stem cells

VIRAL DELIVERY OF GDNF PROMOTES FUNCTIONAL INTEGRATION OF HUMAN STEM CELL GRAFTS IN PARKINSONS DISEASE

This study shows that early viral delivery of GDNF improves survival and function of human stem cell grafts in Parkinson's disease models.

Extraembryonic endoderm cells induce neuroepithelial tissue in gastruloids

The paper shows that adding XEN cells to mouse gastruloids induces neuroepithelial tissue formation through reciprocal differentiation and WNT signaling inhibition.

Single-cell RNA-seq of differentiating iPS cells reveals dynamic genetic effects on gene expression

The paper uses single-cell RNA-seq of differentiating iPS cells from 125 donors to identify expression quantitative trait loci influencing gene expression during endoderm differentiation.

Mesenchymal-Epithelial Transition Regulates Initiation of Pluripotency Exit before Gastrulation

The paper shows that pluripotent stem cells undergo a mesenchymal-epithelial transition (MET) before losing pluripotency, which is distinct from the gastrulation EMT and regulated by integrin-mediated cell-matrix interactions.