Pluripotent Stem Cells Research

Generation of parasympathetic neurons from hiPSC that reproduce the electrophysiological properties of native neurons and modulate the activity of hiPSC-atrial cardiomyocytes

This paper describes a protocol to generate parasympathetic neurons from hiPSC that exhibit native electrophysiological properties and modulate hiPSC-atrial cardiomyocyte activity.

Bovine Formative Embryonic Stem Cell Plasticity in Embryonic and Extraembryonic Differentiation

This paper generates bovine formative embryonic stem cells with dual developmental plasticity, capable of differentiating into various embryonic and extraembryonic cell types.

Lipid Metabolism Remodeling in Human Cardiomyocyte Differentiation and Maturation

This study maps dynamic lipid changes during human cardiomyocyte differentiation using lipidomics and transcriptomics, identifying key lipid species and their roles in cardiac development.

High-Purity Production of Endothelial Cells from Human Pluripotent Stem Cells

This paper presents a method to produce high-purity endothelial cells from human pluripotent stem cells without cell sorting, resulting in cells with arterial-like characteristics comparable to HUVECs.

Parsing glomerular and tubular structure variability in high-throughput kidney organoid culture

This study evaluates variability in kidney organoid structure development across different culture approaches and iPSC lines using high-throughput methods.

A Novel Method to Characterize Functional Human Embryonic Stem Cells Derived Cardiomyocytes

This study evaluates TLR expression patterns in hESC-derived cardiomyocytes to assess their functionality and maturation.

Systematic characterization of existing and novel inducible transgenic systems in human pluripotent stem cells after prolonged differentiation

This study evaluates inducible transgenic systems in human pluripotent stem cells and differentiated cells, identifying a small molecule-mediated splicing switch that enables tunable transgene expression without protein tags.

mTORC2-mediated cell-cell interactions promote BMP4-induced WNT activation and mesoderm differentiation

This study shows mTORC2 is required for BMP4-induced WNT activation and mesoderm differentiation through cell-cell interactions in hESCs.

Calcium: Modulator of Post-transcriptional and post-translational process in mESCs

This study shows that calcium regulates pluripotency, cell cycle, differentiation, and post-translational/post-transcriptional processes in mESCs through pCaMKII-dependent mechanisms.

Novel lentiviral constructs for the specification of rare and ectopic cell types in human colonoids.

This study develops lentiviral constructs to induce specific epithelial cell types in human colonoids, revealing that KLF4 can generate goblet cells and colonocytes, while NEUROG3 and NKX6-3 induce duodenal enteroendocrine subtypes.

Isogenic monocytes improve the responsiveness of hiPSC cardiac spheroids to cardiac stressors

The paper develops a cardiac spheroid model with monocytes to better mimic heart failure responses, showing improved functional and transcriptional changes compared to models without immune cells.

KLF7 is a general inducer of human pluripotency

This study identifies KLF7 as a key inducer of human pluripotency, showing its role in reprogramming somatic cells and enhancing chemical resetting to naive pluripotent stem cells.

Induction of cellular totipotency by NACC1-driven feed-forward regulation.

The paper identifies NACC1 as a regulator of totipotent-like cells in mouse embryonic stem cells through a feed-forward mechanism involving gene regulation and retrotransposon activity.

Transient let-7/miR-98 reprograms primed pluripotency to induce naivehuman PSCs

This paper identifies miR-98-5p as a let-7 family microRNA that enhances naive human PSC induction by repressing NR6A1 and activating BMP2/4 signaling, improving resetting efficiency to ~80%.

Comprehensive profiling of transcription factors for reprogramming human astrocytes to neuronal cells through endogenous CRISPR-based gene activation

The paper identifies transcription factors for reprogramming human astrocytes to neurons using CRISPRa screens and scRNA-seq, showing INSM1's broad neurogenic activity and cofactor interactions.

Multi-Cellular Human Liver Organoids Enable Complete Maturation of Induced Pluripotent Hepatocyte-like Cells Through Purely Endogenous Signals

This study shows that multi-cellular human liver organoids can mature induced pluripotent hepatocyte-like cells through endogenous signals, achieving hepatic marker expression and function similar to primary human hepatocytes.

Development of patient-derived neuroprogenitor cells (hNPCs), neurons, and astrocytes to explore the etiology of Guam Parkinsonism-dementia complex (PDC)

The paper develops patient-derived iPSCs to model ALS-PDC and study environmental and genetic factors in neurodegenerative diseases.

Generation of Valvular Interstitial Cells from Human Pluripotent Stem Cells

This paper describes a method to generate valvular interstitial cells from human pluripotent stem cells, showing high transcriptional similarity to primary fetal VICs and recapitulating disease features in an in vitro model.

Upregulation of the lncRNA XACT sustains pluripotency, blocks lineage specification, and drives germ cell tumor-like transcriptional programs in human pluripotent stem cells

A human-specific regulatory mechanism revealed in a preimplantation model

The paper investigates the role of HERVK LTR5Hs elements in human preimplantation development, showing their cis-regulatory impact on the epiblast transcriptome and their essential role in blastoid formation through ZNF729 activation.

Generation of Region-specific Airway Basal Stem Cells from Human Pluripotent Stem Cells via Regulation of NOGGIN-BMP Axis

This study generates region-specific airway basal stem cells from human pluripotent stem cells by modulating the NOGGIN-BMP axis, showing distinct differentiation potentials for proximal and distal airway cell types.

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells

The paper shows that mechano-osmotic changes in pluripotent stem cells influence chromatin state and fate transitions by integrating mechanical and biochemical signals.

Human DCM-time machine unravels cell state changes during primitive gut tube differentiation

The paper introduces DCM-time machine (DCM-TM) to track gene and enhancer activity during human iPSC differentiation, showing its ability to label active elements and correlate methylation changes with cell state transitions.

Regulation of mouse blastocyst primitive endoderm differentiation by p38-mitogen-activated-kinases (p38-MAPKs) is inner-cell-mass (ICM) autonomous and unrelated to cavity expansion defects in ICM specification.

The paper investigates whether p38-MAPK inhibition affects primitive endoderm differentiation in mouse blastocysts through ICM autonomous mechanisms or cavity expansion defects.

Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip

This study shows that culturing pluripotent stem cells in microscale droplets promotes differentiation into three germ layers and organoid formation, with enhanced tissue patterning and self-organization.

Stable Maintenance of Two-Cell-Like Cells from Embryonic Stem Cells Reveals Chromatin and Super Enhancer Regulation of MERVL Elements

The paper establishes stable 2C-like cells from ESCs, showing persistent MERVL expression and expanded developmental potential through epigenomic and transcriptomic analysis.

Dual-patterned pluripotent stem cells self-organize into a human embryo model with extended anterior-posterior patterning

The paper describes a method to generate human embryo-like structures by pre-patterning stem cells and mixing them, resulting in models with extended anterior-posterior organization and neural features.

Development of a Human iPSC-Derived "Corticospinal Tract-on-a-Chip" for Neurodegenerative Disease Research

This paper develops a human iPSC-derived microfluidic platform to model corticospinal tract connectivity and pathology for neurodegenerative disease research.

Developmental regulation of endothelial-to-hematopoietic transition from induced pluripotent stem cells

The paper maps single-cell dynamics of endothelial-to-hematopoietic transition from iPSCs and identifies FGF signaling modulation to improve hematopoietic output.

Satellite glial contact enhances differentiation and maturation of human iPSC-derived sensory neurons

This study shows that co-culturing human iPSC-derived sensory neurons with rodent satellite glia enhances their differentiation and maturation, leading to more mature phenotypes and improved disease modeling.