Pluripotent Stem Cells Research

Optimizing PSC culture for generating neural organoids

This study identifies factors affecting neural organoid generation by analyzing proteomic data and optimizes culture conditions to improve organoid quality.

Protein-protein interactions drive differences in the spatiotemporal dynamics of transcription factors NANOG and SOX2 in naïve pluripotent cells

The paper tracks SOX2 and NANOG dynamics in live cells using advanced microscopy and analysis pipelines, revealing differences in their spatiotemporal behavior due to protein-protein interactions.

Rapid, Growth Factor-Reduced Differentiation of Functional Neurons from hiPSCs

This paper develops a low-cost protocol to generate functional neurons from hiPSCs in 6 days using a TET-on NGN2 system and DAPT to enhance neurogenesis.

The exit of naïve pluripotency contains a lipid metabolism-induced checkpoint for genome integrity.

The paper identifies a lipid metabolism-induced checkpoint during the exit of naïve pluripotency that ensures genome integrity through telomere regulation.

Rejuvenation capacity of genetic versus human-optimized chemical reprogramming in cellular aging models

This study compares genetic and chemical reprogramming in human fibroblasts, finding that chemical reprogramming matches or exceeds genetic efficacy while producing more homogeneous outcomes and less cellular stress.

Robotic Handling Preserves Induced Pluripotent Stem Cell Derived Vascular Smooth Muscle Cells Differentiation using a Weekend-free, Automatable, Low-variability, Low-cost (WALL) Protocol

This paper develops an automated protocol for differentiating iPSC-derived vascular smooth muscle cells and shows that automation does not compromise cell quality or protein expression.

VGLL1 contributes to both the transcriptome and epigenome of the developing trophoblast compartment

This study identifies VGLL1 as a key regulator of trophoblast development by integrating BMP, WNT, and HIPPO signaling through chromatin remodeling and transcriptional control in human stem cells.

Unified Generation of Regionalized Neural Organoids from Single-Lumen Neuroepithelium

This paper introduces a scalable platform to generate regionally specified neural organoids from uniform single-lumen neuroepithelium, improving reproducibility and fidelity to natural brain development.

Reconstructing the emergence of the human chorion via HIPPO-mediated trophoblast induction

This study uses HIPPO pathway modulation to induce human trophectoderm and reconstruct chorion development in vitro, identifying key cell types and markers.

N6-Methyladenosine Safeguards Mouse and Human Germline Competence

This study shows that reducing N6-methyladenosine (m6A) levels in mouse and human pluripotent cells disrupts germline entry by altering signaling pathways, chromatin states, and gene expression.

N2B27 media formulations influence gastruloid development

This study compares the effects of homemade and commercial N2B27 media on mouse gastruloid development, finding differences in timing, cell number, and gene expression patterns.

Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

This study compares hiPSC-derived intestinal epithelial cells cultured in organoids, Transwells, and intestine-on-chip systems, showing that the latter promotes stronger gene expression related to nutrient and drug metabolism and a more mature state.

Engineering a perfusion bioreactor system for hiPSC-derived progenitor co-culture capturing microglial features in CNS development

This study develops a perfusion bioreactor system to co-culture hiPSC-derived erythromyeloid progenitors with neurospheres, mimicking microglial features in CNS development.

Advancing Human iPSC-Derived Motor Neuron Models Using Glutamatergic Modulators for Synaptic Function Studies

This study improves human iPSC-derived motor neuron synapse formation using optimized differentiation and glutamatergic modulators, resulting in functional and structurally mature neuronal networks.

WNT inhibition primes the transcriptional landscape of mesoderm to initiate a phased ventricular cardiomyocyte specification programme

This study identifies WNT inhibition-dependent and -independent transcriptional regulators guiding human ventricular cardiomyocyte specification using single-cell RNA sequencing.

Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development

This paper captures mouse trophectoderm-like stem cells (TELSCs) that can generate all trophoblast lineages and form trophoblast organoids, providing insights into placental development.

Blood originates in hypoblasts during embryonic development

The paper identifies hypoblasts as the origin of the first blood cells in human and non-human primate embryos, showing they contribute to hemoglobin+ phagocyte-like cells and sustain oxygen levels before heart formation.

Derivation, Characterizations, and Applications of Rabbit Haploid Embryonic Stem Cells

This study derives and characterizes rabbit haploid embryonic stem cells, showing their stable karyotype, pluripotency, and utility for genetic studies and disease modeling.

Lateral plate mesoderm directs human amnion and ventral skin organoid formation

This study generates human ventral skin and amnion-like organoids by adjusting signaling and cellular composition, revealing key developmental pathways and interactions.

Integration of Hematopoietic and Thymus-like Niches in a Human iPSC-derived Bone Marrow Organoid

This paper develops human iPSC-derived bone marrow organoids that support both hematopoiesis and thymus-like T cell differentiation, offering a platform for immune cell therapy and research.

Optimizing cardiac organoid culture to enhance maturation, viability, and cardiotoxicity assessments

This study optimizes cardiac organoid culture conditions by testing seeding density and culture duration, finding that 20,000 cells seeded for seven days yield the most mature, viable, and cardiotoxicity-sensitive organoids.

Intrinsic mechanotransduction during apical constriction licenses lineage competence in pluripotent stem cells

This study shows that mechanical forces during epithelialization in pluripotent stem cells drive nuclear changes that prime neuroectodermal lineage commitment.

Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5

This study generates and characterizes mouse-mouse chimeric neural cells at embryonic day 12.5 using GFP labeling and imaging analysis.

Rapid generation of ventral A9-like dopaminergic neurons from patterned iPSCs

This paper develops a method to rapidly generate ventral A9-like dopaminergic neurons from iPSCs using small molecules and Ascl1 expression, creating functional 3D organoids for Parkinson's disease research.

Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSCs-derived myogenic progenitor cells

The paper shows that modulating the JAK2-STAT3 pathway enhances the yield and maturation of human iPSC-derived myogenic progenitor cells for potential cell therapy.

From blood to pluripotency: Fibrocytes as a reprogrammable somatic cell source for bovine iPSCs

This study identifies fibrocytes as a reprogrammable somatic cell source for bovine iPSCs, showing their molecular identity and successful reprogramming into pluripotent stem cells.

Guided maturation of human neuromuscular organoids via electrical stimulation

This study uses electrical stimulation to enhance the maturation of human neuromuscular organoids, improving their structural and functional properties.

Enhanced iPSC-Cardiomyocyte Maturation via Combined 3D-Culture and Metabolic Cues

This study enhances iPSC-derived cardiomyocyte maturation by combining 3D culture with a metabolic medium, resulting in improved structural, electrophysiological, and functional characteristics.

TET knockout cells transit between pluripotent states and exhibit precocious germline entry

This study shows that TET protein deficiency in mouse ESCs leads to impaired somatic differentiation and accelerated germline entry.

Long-term prevention of aneuploidy in human pluripotent stem cells by fine-tuning GSK3 activity

This study shows that low-dose GSK3 inhibitors prevent aneuploidy in hPSCs by reducing mitotic DNA synthesis and ultra-fine chromosome bridges during long-term culture.