Pancreatic function and diabetes

Direct conversion of somatic cells into insulin-producing-cells by user-defined multiplex-epigenetic-engineering vector (MEEV-b)

This paper describes a method to convert somatic cells into insulin-producing cells using a multiplex-epigenetic-engineering vector that activates specific genes.

Islet Gene View - a tool to facilitate islet research

The paper introduces Islet Gene View (IGW), a platform that integrates gene expression and phenotypic data from pancreatic islets to explore T2D-related gene expression changes and their functional implications.

Adaptation to chronic ER stress enforces pancreatic β-cell plasticity

This study shows chronic ER stress induces β-cell plasticity, but excessive stress leads to identity loss and is linked to T1D progression.

Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells

This study shows glucose induces translational regulation of over 400 mRNAs in human pancreatic beta cells via mTOR and eIF2 pathways, independent of transcriptional changes.

Synaptotagmin 13 orchestrates pancreatic endocrine cell egression and islet morphogenesis

This study identifies Synaptotagmin 13 as a key regulator of endocrine cell egression and islet formation through its role in cell polarity and basement membrane remodeling.

Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas development

The paper identifies tissue-enriched ligands involved in epithelial-endothelial crosstalk during pancreas development using scRNA-seq and computational interactomics.

TET1 dioxygenase is required for FOXA2-associated chromatin remodeling in pancreatic beta-cell differentiation

This study shows TET1 is essential for FOXA2-mediated chromatin remodeling in pancreatic beta-cell differentiation by rescuing differentiation defects and restoring PAX4 hypomethylation.

Pancreatic α and β cells are globally phase-locked

The paper shows that pancreatic α and β cells exhibit globally phase-locked Ca2+ oscillations with tunable phase shifts that set islet oscillation patterns.

Single-cell Transcriptome and Accessible Chromatin Dynamics During Endocrine Pancreas Development

The paper analyzes single-cell transcriptome and chromatin accessibility data to uncover transcription factor networks governing pancreatic endocrine cell lineage specification.

PAX4 loss of function alters human endocrine cell development and influences diabetes risk

This study shows that PAX4 variants reduce beta cell function and increase diabetes risk by impairing insulin secretion and hormone gene expression in human cells.

Glucose-dependent miR-125b is a negative regulator of β-cell function

This study identifies miR-125b-5p as a glucose-regulated miRNA that negatively regulates β-cell function by modulating organelle dynamics and insulin secretion.

Mitofusins Mfn1 and Mfn2 are required in the β-cell to preserve mitochondrial architecture and insulin secretion

This paper shows that deleting Mfn1 and Mfn2 in beta cells impairs mitochondrial structure and insulin secretion, but incretins can partially compensate through EPAC2 signaling.

Control of pancreatic islet function and glucose homeostasis by a novel microexon program misregulated in type 2 diabetes

The paper identifies a microexon program in pancreatic islets regulated by SRRM3, which affects insulin secretion and is linked to type 2 diabetes risk.

Characterization of the secretome, transcriptome and proteome of human β cell line EndoC-βH1

The paper validates EndoC-βH1 as a translational human β cell model using omics and characterizes its secretome, finding it secretes 671 proteins in baseline and 3,278 under stress, with exosome secretion as a major mode.

GP2-enriched pancreatic progenitors give rise to functional beta cells in vivo and eliminate the risk of teratoma formation

This study shows that GP2-enriched pancreatic progenitors generate functional beta-like cells in vivo and prevent teratoma formation.

A microbiota membrane disrupter disseminates to the pancreas and increases β-cell mass

This study identifies BefA, a microbiome-derived protein that promotes β-cell mass by permeabilizing membranes, linking microbiome composition to diabetes risk.

Arginine 65 methylation of Neurogenin 3 by PRMT1 is a prerequisite for development of hESCs into pancreatic endocrine cells

This study shows arginine 65 methylation of NGN3 by PRMT1 is essential for hESCs to differentiate into pancreatic endocrine cells.

Functional, metabolic and transcriptional maturation of stem cell derived beta cells

This study generates and benchmarks functionally mature stem cell-derived islets, showing they closely resemble primary adult islets in transcriptional and functional properties.

Single-Cell Multi-Omic Roadmap of Human Fetal Pancreatic Development

This study maps human fetal pancreatic development using scRNA-Seq and snATAC-Seq, identifying novel cell types and regulatory mechanisms, and highlights gene FEV's role in endocrine differentiation.

DIMT1, a regulator of ribosomal biogenesis, controls beta-cell protein synthesis, mitochondrial function and insulin secretion

This study identifies DIMT1 as a regulator of ribosomal biogenesis in beta-cells, showing its role in mitochondrial function and insulin secretion, with implications for type 2 diabetes.

Phosphatases are predicted to govern prolactin-mediated JAK-STAT signaling in pancreatic beta cells

The paper uses a computational model to show that phosphatase concentrations and prolactin receptor levels influence JAK-STAT signaling in pancreatic beta cells, suggesting targets for diabetes treatment.

Development of a 3D atlas of the embryonic pancreas for topological and quantitative analysis of heterologous cell interactions

The paper develops a 3D atlas of the embryonic pancreas to analyze cell interactions and spatial organization in mouse embryos.

A single-cell atlas of de novo β-cell regeneration reveals the contribution of hybrid β/δ cells to diabetes recovery in zebrafish

This study maps β-cell regeneration in zebrafish using single-cell transcriptomics, identifying hybrid β/δ cells as key contributors to diabetes recovery.

Insulin is expressed by enteroendocrine cells during human fetal development

The paper identifies a subset of fetal enteroendocrine cells expressing insulin and beta cell genes, suggesting an extra-pancreatic source of beta cells.

Bayesian Metamodeling of pancreatic islet architecture and functional dynamics

The paper develops a Bayesian metamodel of pancreatic islet architecture and function by integrating imaging and physiological data with probabilistic graphical models and automation tools.

Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry

This study uses imaging mass cytometry to analyze pancreatic cells in T2D patients and controls, revealing changes in islet structure, cell composition, and immune cell infiltration.

Sex-biased islet β cell dysfunction is caused by the MODY MAFA S64F variant by inducing premature aging and senescence in males

This paper identifies a MAFA S64F variant causing sex-dependent β cell dysfunction and diabetes by accelerating aging and senescence in males.

Combinatorial transcription factor profiles predict mature and functional human islet α and β cells

The paper identifies combinatorial transcription factor expression in human islet cells and shows it correlates with functional maturity and electrophysiological activity.

Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cells

This paper develops SOX9 and INS reporter cell lines to monitor and drive differentiation of human pluripotent stem cells into insulin-producing beta cells.

Integrated experimental-computational analysis of a liver-islet microphysiological system for human-centric diabetes research

The paper develops a computational model of a liver-islet microphysiological system to predict glucose metabolism and translate experimental results to humans.